Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   BKM03_RS26980 Genome accession   NZ_CP026562
Coordinates   5644280..5646385 (-) Length   701 a.a.
NCBI ID   WP_005739571.1    Uniprot ID   -
Organism   Pseudomonas avellanae strain R2leaf     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5639280..5651385
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BKM03_RS26965 (BKM03_26965) - 5640908..5642524 (-) 1617 WP_060402257.1 AAA family ATPase -
  BKM03_RS26970 (BKM03_26970) aroB 5642535..5643638 (-) 1104 WP_005739572.1 3-dehydroquinate synthase -
  BKM03_RS26975 (BKM03_26975) aroK 5643757..5644275 (-) 519 WP_003378847.1 shikimate kinase AroK -
  BKM03_RS26980 (BKM03_26980) pilQ 5644280..5646385 (-) 2106 WP_005739571.1 type IV pilus secretin PilQ Machinery gene
  BKM03_RS26985 (BKM03_26985) pilP 5646433..5646960 (-) 528 WP_003378849.1 type 4a pilus biogenesis lipoprotein PilP -
  BKM03_RS26990 (BKM03_26990) pilO 5646957..5647580 (-) 624 WP_003378850.1 type 4a pilus biogenesis protein PilO -
  BKM03_RS26995 (BKM03_26995) - 5647577..5648197 (-) 621 WP_005739569.1 PilN domain-containing protein -
  BKM03_RS27000 (BKM03_27000) pilM 5648197..5649261 (-) 1065 WP_005739568.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 701 a.a.        Molecular weight: 75385.86 Da        Isoelectric Point: 5.0342

>NTDB_id=270283 BKM03_RS26980 WP_005739571.1 5644280..5646385(-) (pilQ) [Pseudomonas avellanae strain R2leaf]
MTRILSIIGVSLCIAMLSPVLQAANLKTLDVAALPGDRIELKLAFDAPVPAPRGYTTAQPARIALDLPGVTSQLETKSRE
LGTGNARSVVVVQAQDRTRVIINLTTLAPYSSRVDGNNLFVVIGQGAAAAQASQPTTAMGAQRVAVPPPVPASAKPFIPA
GVKAIRNVDFQRGELGEGNVVIDLSNPSIAPDIQEQGGKIRVDFAKTQLPEPLRVRLDVKDFATPVQFVSASATGDKASI
LIEPSGAFDYSAFQTENKLTISVRPLTNEDLDRRAADKPVYTGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGI
TLRLQNVPWDQALDLVLKTKGLDKRKVGSVLLVAPADEIAARERQELESLKQIAELAPLRRELLQVNYAKAADIAKLFQS
VTSAESKAEERGSITVDDRTNNIIAYQTQDRLDELRRIVSQLDIPVRQVMIEARIVEANVDYNKQLGVRWGGSTNTSGSG
KWTTYGLDNNGDEAGNTSGNLTSNVPFVDLGAAGATSGIGLGFVTNNTLLDLELSAMEKTGNGEIVSQPKVVTSDKETAK
ILKGTEIPYQESSSSGATTVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNAVLGVPPIKKNEVNAKVLISDGET
IVIGGVFSNTQSKVVDKVPFLGDVPYLGRLFRRDVVSESKSELLVFLTPRIMNNQAISVSR

Nucleotide


Download         Length: 2106 bp        

>NTDB_id=270283 BKM03_RS26980 WP_005739571.1 5644280..5646385(-) (pilQ) [Pseudomonas avellanae strain R2leaf]
ATGACCCGGATTCTCTCGATTATCGGCGTGTCGTTATGCATAGCGATGCTGTCGCCGGTTCTCCAGGCTGCCAACCTCAA
GACCCTGGATGTCGCCGCCCTGCCAGGCGATCGCATCGAGCTCAAGCTCGCCTTTGACGCACCGGTGCCCGCGCCGCGCG
GCTACACCACCGCGCAGCCGGCGCGCATCGCTCTGGACCTGCCGGGCGTGACCAGCCAGTTGGAGACCAAAAGCCGTGAG
CTGGGCACCGGCAATGCACGCAGCGTAGTGGTGGTGCAGGCTCAGGATCGCACGCGAGTGATCATCAACCTGACGACGCT
TGCGCCTTACAGCTCCCGCGTCGACGGCAACAATCTGTTCGTGGTCATTGGCCAGGGTGCCGCCGCAGCGCAGGCTTCCC
AGCCAACCACTGCGATGGGCGCGCAACGGGTAGCGGTGCCGCCGCCTGTCCCGGCGTCTGCCAAGCCCTTTATACCGGCG
GGCGTCAAAGCCATCCGCAATGTCGATTTTCAGCGCGGTGAGCTGGGTGAAGGCAATGTGGTCATCGACCTGAGTAACCC
GAGCATCGCGCCTGATATTCAGGAGCAGGGCGGCAAGATCCGTGTCGACTTCGCCAAGACCCAGTTGCCCGAGCCTCTGC
GTGTGCGGCTGGACGTCAAGGATTTCGCGACCCCTGTGCAGTTCGTCAGTGCGTCGGCCACGGGCGACAAGGCCAGTATT
CTGATCGAGCCGTCGGGTGCCTTCGATTACTCTGCGTTCCAGACCGAAAACAAGCTGACCATCAGCGTACGTCCGTTGAC
CAACGAAGATCTGGACCGCCGCGCTGCCGACAAGCCGGTGTACACCGGTGAAAAGCTCTCGCTTAACTTCCAGGACATCG
ACGTGCGTTCGGTGCTGCAACTGATCGCCGACTTCACCAACCTCAATCTGGTGGCCAGCGACACGGTTCAGGGCGGCATC
ACCCTGCGCCTGCAGAACGTGCCGTGGGATCAGGCGCTGGACCTGGTGCTGAAAACCAAAGGCCTGGACAAGCGCAAGGT
CGGCAGTGTTCTGCTGGTTGCGCCAGCGGATGAAATTGCCGCCCGCGAACGCCAGGAGCTGGAATCGCTCAAGCAGATTG
CCGAACTGGCACCGCTGCGCCGCGAGCTGCTGCAGGTCAATTACGCCAAGGCAGCCGACATCGCCAAGCTGTTTCAGTCA
GTGACCAGCGCCGAGTCCAAGGCTGAAGAGCGTGGCTCGATCACCGTGGACGACCGCACCAACAATATCATTGCCTACCA
GACCCAGGACCGTCTCGACGAACTGCGTCGCATCGTTTCGCAACTGGACATCCCGGTGCGCCAGGTGATGATCGAAGCAC
GCATCGTCGAGGCCAACGTCGATTACAACAAGCAGCTGGGCGTGCGCTGGGGCGGTTCGACCAACACCAGCGGCAGCGGG
AAATGGACGACTTACGGGCTGGATAACAATGGCGATGAGGCTGGTAACACGAGCGGCAATCTGACTTCCAACGTTCCGTT
CGTGGACCTGGGGGCCGCCGGGGCGACGTCCGGCATCGGTCTGGGCTTTGTGACCAATAACACCTTGCTGGACCTTGAGC
TGAGCGCGATGGAGAAGACCGGCAACGGCGAAATCGTCTCCCAGCCCAAAGTGGTCACGTCCGACAAGGAAACCGCCAAG
ATCCTCAAGGGCACGGAGATTCCGTATCAGGAATCCAGCTCCAGCGGCGCCACCACAGTAAGCTTCAAGGAGGCTTCGCT
GTCGCTTGAAGTCACGCCGCAGATCACGCCGGACAACCGCATCATCATGGAGGTCAAGGTCACCAAGGATGAGCCGGACT
ACCTGAATGCGGTGCTTGGCGTACCGCCGATCAAGAAAAACGAGGTCAACGCCAAAGTGCTGATTTCCGATGGCGAAACC
ATCGTGATCGGCGGTGTGTTCTCCAATACGCAAAGTAAAGTTGTCGATAAAGTGCCATTTCTTGGCGATGTACCGTATCT
TGGCCGGCTTTTCAGGCGCGATGTGGTATCCGAGTCCAAATCCGAGCTGTTGGTATTTTTGACTCCGCGTATCATGAACA
ACCAGGCGATTTCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(383-448)

AMIN domain (AMIN)

(38-122)

Secretin and TonB N terminus short domain (STN)

(309-355)

Bacterial type II and III secretion system protein (Secretin)

(536-692)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.614

100

0.749
  comQ Acinetobacter baylyi ADP1

41.917

99.715

0.418
  pilQ Acinetobacter baumannii D1279779

38.889

100

0.409
  pilQ Acinetobacter baumannii strain A118

38.451

100

0.404
  pilQ Legionella pneumophila strain Lp02

38.986

98.431

0.384
  pilQ Legionella pneumophila strain ERS1305867

38.653

97.432

0.377

Multiple sequence alignment