Detailed information NTG0270284

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: BKM03_RS27000
• Genome accession: NZ_CP026562
• Coordinates: 5648197..5649261 (-)
• Length: 354 a.a.
• NCBI ID: WP_005739568.1
• Uniprot ID: A0A2S4IFK9
• Organism: Pseudomonas avellanae strain R2leaf
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5643197..5654261

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BKM03_RS26975 (BKM03_26975)	aroK	5643757..5644275 (-)	519	WP_003378847.1	shikimate kinase AroK	-
BKM03_RS26980 (BKM03_26980)	pilQ	5644280..5646385 (-)	2106	WP_005739571.1	type IV pilus secretin PilQ	Machinery gene
BKM03_RS26985 (BKM03_26985)	pilP	5646433..5646960 (-)	528	WP_003378849.1	type 4a pilus biogenesis lipoprotein PilP	-
BKM03_RS26990 (BKM03_26990)	pilO	5646957..5647580 (-)	624	WP_003378850.1	type 4a pilus biogenesis protein PilO	-
BKM03_RS26995 (BKM03_26995)	-	5647577..5648197 (-)	621	WP_005739569.1	PilN domain-containing protein	-
BKM03_RS27000 (BKM03_27000)	pilM	5648197..5649261 (-)	1065	WP_005739568.1	pilus assembly protein PilM	Machinery gene
BKM03_RS27005 (BKM03_27005)	-	5649468..5651912 (+)	2445	WP_005739567.1	penicillin-binding protein 1A	-
BKM03_RS27010 (BKM03_27010)	-	5652225..5653493 (-)	1269	WP_003378855.1	malic enzyme-like NAD(P)-binding protein	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38075.57 Da
• Isoelectric Point: 4.7008

>NTDB_id=270284 BKM03_RS27000 WP_005739568.1 5648197..5649261(-) (pilM) [Pseudomonas avellanae strain R2leaf]
MFELFGKKANTLLGIDISSTSVKLLELSRSGTRYKVESYAVEPLPANAVVEKNIAELEGVGQALSRVLAKAKTNVKIVAV
AVAGSAVITKTIEMDAGLSDDDMENQLKLEADQYIPYPLEEVAIDFEVQGYSVRNPERVEVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLAAQLGNGHDELTVAVVDIGATMTTLSVLHHGRIIYTREQLFGGRQLTDEIQRRYGLSM
EEAGLAKKQGGLPDDYVSEVLDPFKDALVQQVSRSLQFFFAAGQYNSVDHIMLAGGTASISGLEHLIQRRIGTPTMVANP
FADMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=270284 BKM03_RS27000 WP_005739568.1 5648197..5649261(-) (pilM) [Pseudomonas avellanae strain R2leaf]
GTGTTCGAACTCTTTGGCAAGAAGGCCAATACCCTTCTAGGGATCGATATTAGCTCCACCTCGGTAAAACTCCTGGAATT
GAGTCGTTCCGGCACTCGTTACAAGGTCGAGTCTTACGCAGTAGAGCCGTTACCGGCCAACGCTGTCGTCGAAAAGAATA
TTGCCGAACTCGAAGGGGTTGGTCAGGCGTTGTCTCGCGTACTGGCCAAGGCCAAGACTAACGTCAAGATTGTGGCGGTT
GCCGTGGCGGGTTCCGCTGTGATCACCAAAACCATCGAGATGGACGCCGGTCTCTCCGACGACGACATGGAAAATCAGCT
TAAGCTTGAAGCTGATCAGTACATCCCTTATCCGCTCGAAGAAGTTGCCATCGATTTTGAAGTGCAGGGCTACTCGGTCC
GCAACCCGGAACGTGTCGAAGTCCTGTTGGCGGCCTGCCGCAAGGAAAACGTCGAGGTGCGCGAGGCTGCGCTGGCTCTC
GCCGGGCTGACGGCGCGTGTCGTGGACGTCGAGGCCTACGCGCTGGAACGTTCATTCGGGCTTCTGGCTGCGCAACTGGG
CAACGGTCATGACGAACTGACCGTTGCCGTGGTGGACATCGGCGCGACCATGACCACCCTCAGCGTGCTGCACCACGGCC
GAATCATCTATACCCGCGAGCAATTGTTCGGCGGCCGCCAGCTGACTGACGAAATTCAGCGCCGCTACGGGCTTTCCATG
GAAGAGGCCGGGCTGGCCAAGAAGCAGGGCGGGCTGCCTGACGATTATGTCAGCGAAGTGCTGGACCCCTTCAAGGACGC
GCTCGTGCAACAGGTTTCCCGATCCCTGCAGTTCTTCTTTGCGGCCGGTCAGTACAACTCCGTGGATCACATCATGCTGG
CAGGCGGTACGGCTTCGATTTCAGGGCTGGAGCATTTGATTCAGCGACGTATCGGCACGCCGACCATGGTTGCCAACCCG
TTTGCCGACATGGCCTTGAGCTCCAAGGTCAATGCCGGTGCGCTGGCCAGCGATGCGCCTGCCCTGATGATCGCGTGCGG
TCTGGCGTTGAGGAGCTTTGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: A0A2S4IFK9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	56.215	100	0.562
comM	Acinetobacter nosocomialis M2	56.215	100	0.562
comM	Acinetobacter baylyi ADP1	53.672	100	0.537
pilM	Legionella pneumophila strain ERS1305867	46.761	100	0.469