Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   C3F36_RS13430 Genome accession   NZ_CP026406
Coordinates   2823214..2824323 (-) Length   369 a.a.
NCBI ID   WP_104455226.1    Uniprot ID   -
Organism   Aeromonas sp. ASNIH2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2818214..2829323
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C3F36_RS13405 (C3F36_13405) - 2818347..2818574 (+) 228 WP_049636474.1 hypothetical protein -
  C3F36_RS13410 (C3F36_13410) brnQ 2818664..2819971 (-) 1308 WP_104456053.1 branched-chain amino acid transport system II carrier protein -
  C3F36_RS13415 (C3F36_13415) - 2820162..2820872 (-) 711 WP_104455224.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  C3F36_RS13420 (C3F36_13420) srmB 2821000..2822220 (+) 1221 WP_041210934.1 ATP-dependent RNA helicase SrmB -
  C3F36_RS13425 (C3F36_13425) yaaA 2822408..2823181 (-) 774 WP_104455225.1 peroxide stress protein YaaA -
  C3F36_RS13430 (C3F36_13430) pilU 2823214..2824323 (-) 1110 WP_104455226.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  C3F36_RS13435 (C3F36_13435) pilT 2824347..2825381 (-) 1035 WP_010673050.1 type IVa pilus ATPase TapT Machinery gene
  C3F36_RS13440 (C3F36_13440) - 2825421..2826122 (+) 702 WP_039039960.1 YggS family pyridoxal phosphate-dependent enzyme -
  C3F36_RS13445 (C3F36_13445) proC 2826245..2827069 (+) 825 WP_104455227.1 pyrroline-5-carboxylate reductase -
  C3F36_RS13450 (C3F36_13450) - 2827093..2827644 (+) 552 WP_010673053.1 YggT family protein -
  C3F36_RS13455 (C3F36_13455) yggU 2827644..2827943 (+) 300 WP_104455228.1 DUF167 family protein YggU -
  C3F36_RS13460 (C3F36_13460) - 2827962..2828381 (+) 420 WP_104455229.1 DUF4426 domain-containing protein -
  C3F36_RS13465 (C3F36_13465) - 2828457..2829287 (-) 831 WP_104455230.1 CPBP family intramembrane glutamic endopeptidase -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 40913.03 Da        Isoelectric Point: 6.3155

>NTDB_id=269154 C3F36_RS13430 WP_104455226.1 2823214..2824323(-) (pilU) [Aeromonas sp. ASNIH2]
MNIDVLLAALVEQKGSDLFITVDAPPTLKVNGRLVSLGPAPLDKTTALALIRESLDETHFERFLHTREANYAIQRGSLGR
FRVSAFWQQDMPGMVLRRIETRIPTFDELVLPPILQEVAMAKRGLVLFVGATGAGKSTTQAAMIGFRNQHGDGHILTVED
PVEFVHQHDRCLVTQREVGIDTASFDVALKNSLRQAPDVILIGEIRSQETMEFAIQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQFLFDLSFNLRAIVAQQLLPSAHGQRRVAAFEILLNTPLVTDIIRKGEMHRLKEVMTKSGELGMQTFDQA
LFTLFCTGQIGYSEALAHADSANDLRLLIKLSGREQLGAGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=269154 C3F36_RS13430 WP_104455226.1 2823214..2824323(-) (pilU) [Aeromonas sp. ASNIH2]
ATGAACATAGATGTTCTGCTCGCCGCACTGGTTGAACAAAAGGGCTCGGATCTCTTCATCACGGTCGATGCTCCCCCGAC
CCTCAAGGTCAACGGGCGTCTGGTGTCGCTCGGGCCGGCTCCCCTCGACAAGACGACGGCGCTGGCGTTGATCAGAGAAA
GCCTGGACGAGACCCACTTCGAGCGCTTCCTGCACACCCGCGAGGCCAACTACGCCATCCAGCGAGGCTCGCTGGGGCGC
TTTCGGGTCAGCGCCTTCTGGCAACAGGATATGCCGGGCATGGTACTGCGCCGCATCGAGACCCGCATCCCCACCTTTGA
CGAGCTGGTGCTGCCTCCCATCCTGCAGGAGGTCGCCATGGCCAAACGCGGGCTGGTGCTCTTCGTCGGCGCCACTGGCG
CCGGCAAGTCCACCACCCAGGCGGCCATGATAGGCTTTCGCAACCAGCACGGTGACGGCCACATCCTGACGGTGGAGGAT
CCGGTGGAGTTCGTCCATCAGCACGATCGCTGTCTGGTGACCCAGCGGGAGGTGGGGATCGACACCGCATCCTTCGATGT
CGCCCTCAAAAACTCCCTGCGCCAGGCCCCGGATGTCATCCTCATCGGTGAAATCCGCTCCCAGGAGACCATGGAGTTCG
CCATCCAGTTCGCCGAGACGGGGCACCTCTGCCTCGCCACCTTGCACGCCAACAATGCCAACCAGGCGCTGGATCGCATC
CTGCATCTGGTCCCCCAGGAGAAGCACCGCCAGTTCCTGTTCGATCTCTCCTTCAACCTGCGCGCCATCGTCGCCCAGCA
GCTGCTCCCCTCCGCTCACGGGCAGCGCCGGGTCGCCGCCTTCGAGATCCTGCTCAACACCCCGCTCGTCACCGACATCA
TTCGCAAGGGAGAGATGCACAGACTCAAGGAGGTGATGACCAAATCCGGCGAGCTTGGCATGCAGACCTTCGATCAGGCG
CTGTTCACCCTGTTCTGCACCGGCCAAATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCCGCCAACGACCTGCGACT
GCTCATCAAGCTGTCGGGTCGCGAGCAGCTGGGCGCAGGCACTCTCGACAACGTGACCCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.598

99.729

0.604
  pilU Pseudomonas stutzeri DSM 10701

58.974

95.122

0.561
  pilU Acinetobacter baylyi ADP1

57.79

95.664

0.553
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.183

91.87

0.388
  pilT Legionella pneumophila strain Lp02

41.108

92.954

0.382
  pilT Legionella pneumophila strain ERS1305867

41.108

92.954

0.382
  pilT Pseudomonas aeruginosa PAK

38.889

92.683

0.36

Multiple sequence alignment