Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   C3F36_RS07775 Genome accession   NZ_CP026406
Coordinates   1685296..1687002 (+) Length   568 a.a.
NCBI ID   WP_104454847.1    Uniprot ID   -
Organism   Aeromonas sp. ASNIH2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1680296..1692002
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C3F36_RS07745 (C3F36_07745) pdhR 1681453..1682217 (-) 765 WP_010675815.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  C3F36_RS07755 (C3F36_07755) ampD 1682494..1683066 (-) 573 WP_104454845.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  C3F36_RS07760 (C3F36_07760) - 1683195..1683665 (+) 471 WP_104454846.1 retropepsin-like aspartic protease -
  C3F36_RS07765 (C3F36_07765) nadC 1683669..1684532 (+) 864 WP_041213995.1 carboxylating nicotinate-nucleotide diphosphorylase -
  C3F36_RS07770 (C3F36_07770) tapA 1684843..1685292 (+) 450 WP_324806168.1 type IVa pilus major pilin TapA -
  C3F36_RS07775 (C3F36_07775) pilB 1685296..1687002 (+) 1707 WP_104454847.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  C3F36_RS07780 (C3F36_07780) pilC 1687130..1688371 (+) 1242 WP_010675822.1 type II secretion system F family protein Machinery gene
  C3F36_RS07785 (C3F36_07785) pilD 1688449..1689324 (+) 876 WP_104454848.1 A24 family peptidase Machinery gene
  C3F36_RS07790 (C3F36_07790) coaE 1689343..1689957 (+) 615 WP_104454849.1 dephospho-CoA kinase -
  C3F36_RS07795 (C3F36_07795) zapD 1689995..1690717 (+) 723 WP_010675825.1 cell division protein ZapD -
  C3F36_RS07800 (C3F36_07800) yacG 1690728..1690922 (+) 195 WP_039040499.1 DNA gyrase inhibitor YacG -
  C3F36_RS07805 (C3F36_07805) mutT 1690980..1691384 (-) 405 WP_174213873.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62550.90 Da        Isoelectric Point: 5.8214

>NTDB_id=269141 C3F36_RS07775 WP_104454847.1 1685296..1687002(+) (pilB) [Aeromonas sp. ASNIH2]
MTSSPNSGLATSLAASSLLSEPDSQRYLSQAKAQRKPFVTFLIENDILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEESKLTAAIGKLMESEQDTLGMDHIDEAEI
SELEVSDESSRLDESVNTADDDAPIVKYIHKIMMDAIKRGASDLHFEPYETKYRIRFRVDGILHEVATPPVNLANRFAAR
LKVMARLDIAERRLPQDGRIKLKISRNRSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIDQLGFDDRQKTQYLHALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTSEVNISTAEDPVEINLPGVNQVQINPKAGLTFASALRSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
QQQLAAGLRLFKPVGCKECSGGYRGRVGIYEIMLMSDNIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EINRITTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=269141 C3F36_RS07775 WP_104454847.1 1685296..1687002(+) (pilB) [Aeromonas sp. ASNIH2]
ATGACCTCAAGTCCCAATAGCGGCCTGGCCACCAGCCTGGCCGCTTCTTCCCTCCTCAGTGAGCCGGACTCACAGCGCTA
TCTGAGCCAGGCCAAGGCGCAGCGCAAGCCCTTTGTCACCTTCCTGATTGAAAACGACATCCTCGACAGCAAAGCCCTCG
CCGATTTCTGCGAGCTGGAATATGGGGTACCTCTGCTGGATCTGGCGGCCTTTGATCTCGCCGAGATCCCGCAGAAATAC
CTCAATCAAAAGCTGATTGAAAAGCACCATGTCCTGCCCATCTACACCCAGGGGCATACTCTCTATATCGCCATGTCCGA
TCCGACCAATGTGTCGGCTCTGGAGGATTTTGGCTTCAGCTTCGGCCTGCATACGGAAGCCCTGCTGGTCGAAGAGAGCA
AGCTGACCGCCGCCATCGGCAAGCTGATGGAGAGCGAGCAGGATACCCTGGGCATGGATCATATCGACGAGGCCGAGATC
TCCGAACTCGAGGTCTCCGACGAGAGTTCCCGCCTCGACGAGAGCGTCAACACCGCGGACGATGACGCCCCCATCGTCAA
ATACATCCACAAGATCATGATGGATGCCATCAAGCGCGGGGCCTCCGACCTGCATTTCGAACCCTACGAGACCAAGTACC
GAATCCGCTTTCGGGTAGACGGCATACTGCACGAAGTCGCCACGCCGCCGGTCAACCTGGCCAACCGCTTCGCGGCTCGC
CTCAAGGTGATGGCGCGGCTCGACATTGCCGAACGGCGCCTGCCCCAGGATGGCCGCATCAAGCTCAAGATCTCGCGCAA
CAGATCCATGGACATGCGGGTCAACACCCTGCCCACCATGTGGGGCGAAAAGATCGTGATCCGGCTGCTGGACTCCTCGG
CCGCCCGACTCAATATCGATCAGCTCGGCTTTGACGACCGGCAGAAGACGCAATACCTGCACGCCCTCTCCAAACCTCAG
GGCATGATCCTGGTCACCGGTCCCACGGGGTCTGGCAAGACGGTCTCACTCTATACCGGCCTCAACATTCTCAACACCAG
CGAGGTCAATATCTCCACCGCCGAAGATCCGGTTGAGATAAACCTGCCCGGCGTCAACCAGGTGCAGATCAATCCCAAAG
CAGGGCTCACCTTCGCCAGCGCCCTGCGCTCCTTCCTGCGCCAGGATCCCGACATCGTCATGGTGGGGGAGATACGGGAT
CTGGAAACCGCCGAGATCGCCATCAAGGCCGCCCAGACCGGTCACCTGGTGCTCTCCACCCTGCACACCAACTCGGCGGC
AGAAACGCTCACCCGGATGATGAACATGGGGGTGCCGGCCTTCAACATCGCCTCTTCGGTCACCCTCATCATGGCCCAGC
GCCTTGCCCGCAAACTCTGCGATCACTGCAAGGCGCCGGAAGTGGTGCCGGAAGCCGAGTTGCTGGAGCTGGGCTTCACC
CAGCAGCAACTGGCCGCCGGGTTGCGGCTGTTCAAGCCCGTGGGATGCAAGGAGTGCTCGGGAGGCTACAGAGGCCGGGT
CGGCATCTATGAGATCATGCTGATGTCCGACAACATCGCCAAACTCATCATGCAGGGCGCCAACTCGCTGCAAATCGCGG
CCATCGCCCAGAAGGAAGGGATGCGCACCCTGCGCACCTCCGGCCTTGAAAAAGCCCGCCTGGGAGTCACCAGCCTGGCC
GAAATCAACAGGATCACCACCAACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.737

100

0.549
  pilB Acinetobacter baumannii D1279779

55.026

99.824

0.549
  pilB Legionella pneumophila strain ERS1305867

54.867

99.472

0.546
  pilB Vibrio cholerae strain A1552

53.415

100

0.537
  pilB Vibrio campbellii strain DS40M4

50.53

99.648

0.504
  pilB Vibrio parahaemolyticus RIMD 2210633

50.178

98.944

0.496
  pilF Neisseria gonorrhoeae MS11

46.809

99.296

0.465
  pilF Thermus thermophilus HB27

40.152

92.958

0.373
  pilB/pilB1 Synechocystis sp. PCC 6803

37.975

97.359

0.37

Multiple sequence alignment