Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilG   Type   Machinery gene
Locus tag   C2124_RS03330 Genome accession   NZ_CP026307
Coordinates   713707..714972 (-) Length   421 a.a.
NCBI ID   WP_174265042.1    Uniprot ID   -
Organism   Ralstonia solanacearum strain IBSBF 2571     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 708707..719972
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C2124_RS03300 (C2124_03310) - 709772..710656 (+) 885 WP_014616064.1 ATP-binding protein -
  C2124_RS03305 (C2124_03315) - 710634..711095 (+) 462 WP_014616065.1 NUDIX domain-containing protein -
  C2124_RS03310 (C2124_03320) - 711088..711303 (-) 216 WP_003266032.1 DNA gyrase inhibitor YacG -
  C2124_RS03315 (C2124_03325) zapD 711320..712078 (-) 759 WP_003266031.1 cell division protein ZapD -
  C2124_RS03320 (C2124_03330) coaE 712196..712810 (-) 615 WP_174265040.1 dephospho-CoA kinase -
  C2124_RS03325 (C2124_03335) pilD 712823..713704 (-) 882 WP_014616067.1 prepilin peptidase Machinery gene
  C2124_RS03330 (C2124_03340) pilG 713707..714972 (-) 1266 WP_174265042.1 type II secretion system F family protein Machinery gene
  C2124_RS03335 (C2124_03345) pilF 715017..716744 (-) 1728 WP_014616069.1 type IV-A pilus assembly ATPase PilB Machinery gene
  C2124_RS03340 (C2124_03350) - 716992..718269 (-) 1278 WP_014616070.1 HlyC/CorC family transporter -
  C2124_RS03350 (C2124_03360) ispB 718544..719512 (-) 969 WP_003271627.1 octaprenyl diphosphate synthase -

Sequence

Protein


Download         Length: 421 a.a.        Molecular weight: 46415.40 Da        Isoelectric Point: 10.4590

>NTDB_id=268043 C2124_RS03330 WP_174265042.1 713707..714972(-) (pilG) [Ralstonia solanacearum strain IBSBF 2571]
MATRAPTANRSAAASARKTASTKAPTQYIFEWEGKDRKGKAFKGEMRAESITEVNAVLRKQGLSITKSKRRRAARGKKIT
PKDVAYFTRQLSTMLKAGVPLLQSVDIIARGHANPNFTQLLTEIRVDIESGSSMAQAFRRHPKYFDSLYCNLIDAGEQGG
ILDALLERLSMYMEKSIALKAQIKSAMIYPISVLTVAFLVTVVLMIFVVPAFKNVFASFGATLPAPTLVVMNISDFFVKW
WYIIIFAPVVGIFLYARAFKRSLKVQRNTHKAILKMPIFGSIIRKATIARWTRTLATMFSAGTPLVESMESVAGAAGNXV
YHDATLEIQQAVRIGTSLTNAMQATHVFDNMVLQMTQIGEESGALDNMLLKVAEFYEREVDDAVANISALIEPIIIVILG
VLIGGMVVAMYLPIFKLGQVV

Nucleotide


Download         Length: 1266 bp        

>NTDB_id=268043 C2124_RS03330 WP_174265042.1 713707..714972(-) (pilG) [Ralstonia solanacearum strain IBSBF 2571]
ATGGCCACACGAGCACCGACCGCGAACCGGTCCGCCGCCGCATCGGCACGCAAAACCGCCTCCACGAAGGCGCCCACGCA
GTACATCTTCGAGTGGGAAGGCAAGGACCGCAAAGGCAAAGCCTTCAAGGGCGAGATGCGGGCGGAGAGCATCACCGAAG
TCAACGCGGTGCTGCGCAAGCAGGGGCTGTCGATCACCAAGTCCAAGCGTCGGCGCGCGGCGCGCGGCAAGAAGATCACG
CCCAAGGACGTGGCCTACTTCACGCGCCAGTTGTCGACCATGCTGAAGGCCGGGGTGCCGCTGCTGCAATCGGTCGACAT
CATCGCGCGCGGCCACGCCAACCCGAACTTCACCCAGTTGCTGACGGAAATCCGCGTCGACATCGAGTCGGGCAGCAGCA
TGGCGCAGGCTTTCCGCCGCCATCCGAAGTACTTCGATTCGCTGTACTGCAACCTGATCGACGCGGGCGAACAGGGCGGT
ATTCTCGACGCGCTGCTCGAGCGGCTGTCGATGTACATGGAAAAGTCGATCGCGCTGAAGGCGCAGATCAAGTCGGCCAT
GATCTACCCAATCTCGGTGCTGACGGTGGCCTTCCTGGTCACGGTGGTGCTGATGATCTTCGTAGTGCCGGCGTTCAAGA
ACGTGTTCGCCAGCTTTGGCGCCACGCTGCCGGCACCGACGCTGGTGGTGATGAATATCTCGGACTTCTTCGTGAAGTGG
TGGTACATCATCATCTTCGCCCCCGTCGTCGGCATCTTCCTGTATGCGCGGGCCTTCAAGCGCTCGCTGAAGGTGCAGCG
CAACACGCACAAGGCCATCCTGAAGATGCCGATCTTCGGCAGCATCATCCGCAAGGCCACCATCGCGCGCTGGACGCGCA
CGCTGGCGACGATGTTCTCCGCCGGTACGCCGCTRGTGGAATCGATGGAGTCGGTGGCCGGTGCGGCCGGCAACTGRGTC
TACCACGACGCCACCCTCGAGATCCAGCAGGCGGTACGCATCGGTACCAGCCTCACCAACGCCATGCAGGCCACGCACGT
GTTCGACAACATGGTGCTGCAGATGACGCAGATCGGCGAGGAATCCGGTGCGCTGGACAACATGCTGCTGAAGGTGGCCG
AGTTCTACGAGCGCGAGGTCGATGACGCGGTAGCCAACATCTCCGCCCTGATCGAGCCGATCATCATCGTGATCCTCGGC
GTGCTGATCGGCGGCATGGTGGTCGCCATGTACCTGCCGATTTTCAAGCTGGGCCAGGTGGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(87-210)

Type II secretion system (T2SS), protein F (T2SSF)

(291-413)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilG Neisseria gonorrhoeae MS11

51.128

94.774

0.485
  pilG Neisseria meningitidis 44/76-A

50.877

94.774

0.482
  pilC Pseudomonas stutzeri DSM 10701

49.751

95.487

0.475
  pilC Acinetobacter baumannii D1279779

47.42

96.675

0.458
  pilC Legionella pneumophila strain ERS1305867

46.914

96.2

0.451
  pilC Acinetobacter baylyi ADP1

46.437

96.675

0.449
  pilC Vibrio campbellii strain DS40M4

38.537

97.387

0.375
  pilC Vibrio cholerae strain A1552

39.25

95.012

0.373

Multiple sequence alignment