Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   C2U47_RS13020 Genome accession   NZ_CP026226
Coordinates   2775877..2776986 (-) Length   369 a.a.
NCBI ID   WP_042882153.1    Uniprot ID   A0A2L0TPJ0
Organism   Aeromonas sp. ASNIH7     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2770877..2781986
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C2U47_RS12995 (C2U47_12995) - 2771011..2771238 (+) 228 WP_069784315.1 hypothetical protein -
  C2U47_RS13000 (C2U47_13000) brnQ 2771328..2772635 (-) 1308 WP_039040015.1 branched-chain amino acid transport system II carrier protein -
  C2U47_RS13005 (C2U47_13005) - 2772826..2773536 (-) 711 WP_069784314.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  C2U47_RS13010 (C2U47_13010) srmB 2773664..2774884 (+) 1221 WP_041210934.1 ATP-dependent RNA helicase SrmB -
  C2U47_RS13015 (C2U47_13015) yaaA 2775071..2775844 (-) 774 WP_042014926.1 peroxide stress protein YaaA -
  C2U47_RS13020 (C2U47_13020) pilU 2775877..2776986 (-) 1110 WP_042882153.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  C2U47_RS13025 (C2U47_13025) pilT 2777010..2778044 (-) 1035 WP_010673050.1 type IVa pilus ATPase TapT Machinery gene
  C2U47_RS13030 (C2U47_13030) - 2778084..2778785 (+) 702 WP_042864460.1 YggS family pyridoxal phosphate-dependent enzyme -
  C2U47_RS13035 (C2U47_13035) proC 2778910..2779734 (+) 825 WP_069784313.1 pyrroline-5-carboxylate reductase -
  C2U47_RS13040 (C2U47_13040) - 2779758..2780309 (+) 552 WP_010673053.1 YggT family protein -
  C2U47_RS13045 (C2U47_13045) yggU 2780309..2780608 (+) 300 WP_010673054.1 DUF167 family protein YggU -
  C2U47_RS13050 (C2U47_13050) - 2780627..2781046 (+) 420 WP_029313762.1 DUF4426 domain-containing protein -
  C2U47_RS13055 (C2U47_13055) - 2781122..2781952 (-) 831 WP_069784312.1 CPBP family intramembrane glutamic endopeptidase -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 40901.04 Da        Isoelectric Point: 6.3155

>NTDB_id=267922 C2U47_RS13020 WP_042882153.1 2775877..2776986(-) (pilU) [Aeromonas sp. ASNIH7]
MNMDVLLAALVEQKGSDLFITVDAPPTLKVNGRLVSLGPAPLDKTTALALIRESLDETHFERFLHTREANYAIQRGSLGR
FRVSAFWQQDMPGMVLRRIETRIPTFDELVLPPILQEVAMAKRGLVLFVGATGAGKSTTQAAMIGFRNQHGDGHILTVED
PVEFVHQHDRCLVTQREVGIDTASFDVALKNSLRQAPDVILIGEIRSQETMEFAIQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQFLFDLSFNLRAIVAQQLLPSAHGQRRVAAFEILLNTPLVTDIIRKGEMHRLKEVMTKSGELGMQTFDQA
LFTLFCAGQIGYSEALAHADSANDLRLLIKLSGREQLGAGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=267922 C2U47_RS13020 WP_042882153.1 2775877..2776986(-) (pilU) [Aeromonas sp. ASNIH7]
ATGAACATGGATGTTCTGCTCGCCGCACTGGTTGAACAAAAGGGCTCGGATCTCTTCATCACGGTCGATGCTCCCCCGAC
CCTCAAGGTCAACGGGCGTCTGGTGTCGCTCGGACCAGCCCCCCTCGACAAGACGACGGCGCTGGCGTTGATCAGGGAGA
GCCTGGACGAGACCCACTTCGAACGCTTCCTGCACACCCGCGAGGCCAACTACGCCATCCAGCGAGGCTCGCTGGGGCGC
TTTCGGGTCAGCGCCTTCTGGCAACAGGATATGCCGGGCATGGTGCTGCGCCGCATCGAGACCCGCATCCCCACCTTTGA
CGAGCTGGTGCTGCCTCCCATCCTGCAGGAGGTCGCCATGGCCAAACGCGGGCTGGTGCTCTTCGTCGGCGCCACTGGCG
CCGGCAAGTCCACCACCCAGGCGGCCATGATAGGCTTTCGCAACCAGCACGGTGACGGCCACATCCTGACGGTGGAGGAT
CCGGTGGAGTTCGTCCATCAGCACGATCGCTGTCTGGTGACCCAGCGGGAGGTGGGGATCGACACCGCATCCTTCGATGT
CGCCCTCAAAAACTCCCTGCGCCAGGCCCCGGATGTCATCCTCATTGGTGAAATCCGCTCCCAGGAGACCATGGAGTTCG
CCATCCAGTTCGCCGAGACGGGACACCTCTGCCTCGCCACCTTGCACGCCAACAATGCCAACCAGGCGCTGGATCGCATC
CTGCATCTGGTCCCCCAGGAGAAGCACCGCCAGTTCCTGTTCGATCTCTCCTTCAACCTGCGCGCCATCGTCGCCCAGCA
GCTGCTCCCCTCCGCTCACGGGCAGCGCCGGGTCGCCGCCTTCGAGATCCTGCTCAACACCCCGCTCGTCACCGACATCA
TTCGCAAGGGAGAGATGCACAGACTCAAGGAGGTGATGACCAAATCCGGCGAGCTCGGCATGCAGACCTTCGATCAGGCA
CTGTTCACCCTGTTCTGCGCCGGCCAAATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCCGCCAACGACCTGCGACT
GCTCATCAAGCTGTCGGGTCGCGAGCAGCTGGGCGCAGGCACTCTCGACAACGTGACCCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2L0TPJ0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.598

99.729

0.604
  pilU Pseudomonas stutzeri DSM 10701

58.974

95.122

0.561
  pilU Acinetobacter baylyi ADP1

57.79

95.664

0.553
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.857

91.057

0.39
  pilT Legionella pneumophila strain Lp02

40.816

92.954

0.379
  pilT Legionella pneumophila strain ERS1305867

40.816

92.954

0.379

Multiple sequence alignment