Detailed information    

insolico Bioinformatically predicted

Overview

Name   clpP   Type   Regulator
Locus tag   C0971_RS15870 Genome accession   NZ_CP026143
Coordinates   3092972..3093577 (+) Length   201 a.a.
NCBI ID   WP_003349416.1    Uniprot ID   I3DU99
Organism   Bacillus methanolicus strain DFS2     
Function   degradation of ComK; degradation of DegU (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 3052626..3093577 3092972..3093577 within 0


Gene organization within MGE regions


Location: 3052626..3093577
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C0971_RS15565 - 3052626..3052769 (-) 144 WP_248892216.1 hypothetical protein -
  C0971_RS15570 (C0971_15650) - 3052861..3053610 (-) 750 WP_248892217.1 N-acetylmuramoyl-L-alanine amidase -
  C0971_RS15575 (C0971_15655) - 3053650..3053895 (-) 246 WP_248892218.1 hypothetical protein -
  C0971_RS15580 (C0971_15660) - 3053897..3054100 (-) 204 WP_283108075.1 hemolysin XhlA family protein -
  C0971_RS15585 (C0971_15665) - 3054108..3054305 (-) 198 WP_248892220.1 hypothetical protein -
  C0971_RS15590 (C0971_15670) - 3054343..3054546 (-) 204 WP_248892221.1 hypothetical protein -
  C0971_RS15595 (C0971_15675) - 3054580..3057537 (-) 2958 WP_248892222.1 phage tail spike protein -
  C0971_RS15600 (C0971_15680) - 3057534..3058964 (-) 1431 WP_248892223.1 distal tail protein Dit -
  C0971_RS15605 (C0971_15685) - 3058968..3063242 (-) 4275 WP_248892224.1 phage tail tape measure protein -
  C0971_RS15610 - 3063258..3063425 (-) 168 WP_248892225.1 hypothetical protein -
  C0971_RS15615 (C0971_15690) - 3063437..3063793 (-) 357 WP_248892226.1 hypothetical protein -
  C0971_RS15620 (C0971_15695) - 3063842..3064408 (-) 567 WP_248892227.1 major tail protein -
  C0971_RS15625 (C0971_15700) - 3064412..3064780 (-) 369 WP_248892228.1 DUF3168 domain-containing protein -
  C0971_RS15630 (C0971_15705) - 3064777..3065163 (-) 387 WP_248892229.1 HK97-gp10 family putative phage morphogenesis protein -
  C0971_RS15635 (C0971_15710) - 3065163..3065483 (-) 321 WP_248892230.1 phage head closure protein -
  C0971_RS15640 (C0971_15715) - 3065498..3065698 (-) 201 WP_248892231.1 hypothetical protein -
  C0971_RS15645 (C0971_15720) - 3065695..3065982 (-) 288 WP_248892232.1 head-tail connector protein -
  C0971_RS15650 (C0971_15725) - 3066000..3067133 (-) 1134 WP_248892233.1 phage major capsid protein -
  C0971_RS15655 (C0971_15730) - 3067130..3067864 (-) 735 WP_248894018.1 head maturation protease, ClpP-related -
  C0971_RS15660 (C0971_15735) - 3067881..3069107 (-) 1227 WP_248892234.1 phage portal protein -
  C0971_RS15665 (C0971_15740) - 3069125..3070864 (-) 1740 WP_248892235.1 terminase large subunit -
  C0971_RS15670 (C0971_15745) - 3070833..3071147 (-) 315 WP_248892236.1 P27 family phage terminase small subunit -
  C0971_RS15675 (C0971_15750) - 3071250..3071570 (-) 321 WP_248892237.1 HNH endonuclease -
  C0971_RS15680 (C0971_15755) - 3071613..3072374 (-) 762 WP_248892238.1 hypothetical protein -
  C0971_RS15685 (C0971_15760) - 3072464..3072679 (-) 216 WP_248892239.1 hypothetical protein -
  C0971_RS15690 (C0971_15765) - 3072828..3073286 (-) 459 WP_248892240.1 DNA-binding response regulator -
  C0971_RS15695 (C0971_15770) - 3073404..3073610 (-) 207 WP_248892241.1 hypothetical protein -
  C0971_RS15700 (C0971_15775) - 3073729..3074016 (-) 288 WP_248892242.1 hypothetical protein -
  C0971_RS15705 (C0971_15780) - 3074045..3074227 (-) 183 WP_248892243.1 DUF3954 domain-containing protein -
  C0971_RS15710 (C0971_15785) - 3074387..3074767 (+) 381 WP_248892244.1 YmaF family protein -
  C0971_RS18295 (C0971_15790) - 3075253..3075666 (+) 414 WP_283108078.1 CBO0543 family protein -
  C0971_RS15715 (C0971_15795) - 3076068..3076394 (+) 327 WP_248892245.1 HNH endonuclease signature motif containing protein -
  C0971_RS15720 - 3076838..3077353 (+) 516 WP_248892246.1 hypothetical protein -
  C0971_RS15725 (C0971_15810) - 3078077..3078370 (-) 294 WP_248892247.1 IDEAL domain-containing protein -
  C0971_RS15730 (C0971_15815) - 3078746..3079021 (+) 276 WP_248892248.1 CotD family spore coat protein -
  C0971_RS15735 - 3079113..3079187 (-) 75 WP_248894019.1 hypothetical protein -
  C0971_RS15740 - 3079157..3079369 (-) 213 WP_248892249.1 hypothetical protein -
  C0971_RS15745 (C0971_15825) - 3079848..3080264 (-) 417 WP_248892250.1 RusA family crossover junction endodeoxyribonuclease -
  C0971_RS15750 - 3080290..3080448 (-) 159 WP_248892251.1 hypothetical protein -
  C0971_RS15755 (C0971_15830) - 3080583..3080765 (-) 183 WP_248892252.1 hypothetical protein -
  C0971_RS15760 - 3080765..3080938 (-) 174 WP_248892253.1 hypothetical protein -
  C0971_RS15765 (C0971_15835) - 3080938..3081267 (-) 330 WP_248892254.1 dUTPase -
  C0971_RS15770 - 3081264..3081437 (-) 174 WP_248892255.1 hypothetical protein -
  C0971_RS15775 (C0971_15840) - 3081618..3082409 (-) 792 WP_248892256.1 hypothetical protein -
  C0971_RS15780 - 3082416..3082592 (-) 177 WP_248892257.1 hypothetical protein -
  C0971_RS15785 (C0971_15845) - 3082585..3083412 (-) 828 WP_248892258.1 ATP-binding protein -
  C0971_RS15790 (C0971_15850) - 3083366..3084337 (-) 972 WP_248892259.1 hypothetical protein -
  C0971_RS15795 - 3084351..3084518 (-) 168 WP_248892260.1 hypothetical protein -
  C0971_RS15800 (C0971_15855) recT 3084515..3085387 (-) 873 WP_248892261.1 recombination protein RecT -
  C0971_RS15805 (C0971_15860) - 3085404..3086306 (-) 903 WP_248894020.1 lambda-exonuclease family protein -
  C0971_RS15810 (C0971_15865) - 3086438..3086653 (-) 216 WP_248891555.1 hypothetical protein -
  C0971_RS15815 - 3086655..3086819 (-) 165 WP_248892262.1 hypothetical protein -
  C0971_RS15820 (C0971_15870) - 3087142..3087654 (-) 513 WP_248892263.1 helix-turn-helix transcriptional regulator -
  C0971_RS15825 (C0971_15875) - 3087729..3088031 (-) 303 WP_248892264.1 DNA-binding protein -
  C0971_RS15830 (C0971_15880) - 3088107..3088379 (-) 273 WP_248892265.1 helix-turn-helix transcriptional regulator -
  C0971_RS15835 (C0971_15885) - 3088407..3088637 (-) 231 WP_248892266.1 helix-turn-helix transcriptional regulator -
  C0971_RS15840 (C0971_15890) - 3088805..3089182 (+) 378 WP_248892267.1 helix-turn-helix transcriptional regulator -
  C0971_RS18300 (C0971_15895) - 3089344..3090669 (+) 1326 WP_283108082.1 thermonuclease family protein -
  C0971_RS15855 (C0971_15900) - 3090760..3091245 (+) 486 WP_248892268.1 ImmA/IrrE family metallo-endopeptidase -
  C0971_RS15860 (C0971_15905) - 3091368..3092519 (+) 1152 WP_248892269.1 site-specific integrase -
  C0971_RS15870 (C0971_15915) clpP 3092972..3093577 (+) 606 WP_003349416.1 ATP-dependent Clp endopeptidase proteolytic subunit ClpP Regulator

Sequence

Protein


Download         Length: 201 a.a.        Molecular weight: 22219.59 Da        Isoelectric Point: 5.0569

>NTDB_id=267536 C0971_RS15870 WP_003349416.1 3092972..3093577(+) (clpP) [Bacillus methanolicus strain DFS2]
MNLIPTVIEQTNRGERAYDIYSRLLKDRIIMLGTPIDDYVANSIVAQLLFLEAENPEKDISIYINSPGGSITAGMAIYDT
MQFIKPDVQTICIGMAASMGAFLLAAGTKGKRYALPNSEVMIHQPLGGAQGQATEIEIAAKRILFLRDKLNQILSERTGQ
PVEVIAKDTDRDNFMTAQQALEYGLVDHIITRNSLEDKSKK

Nucleotide


Download         Length: 606 bp        

>NTDB_id=267536 C0971_RS15870 WP_003349416.1 3092972..3093577(+) (clpP) [Bacillus methanolicus strain DFS2]
ATGAATTTAATTCCTACAGTTATTGAACAAACAAACCGGGGAGAGCGTGCATATGACATTTACTCCCGCCTACTTAAAGA
CCGTATTATTATGCTTGGAACCCCAATTGATGATTATGTAGCGAACTCAATTGTTGCCCAGCTGCTGTTCCTTGAAGCTG
AAAACCCTGAAAAAGATATCTCTATCTATATCAACAGCCCTGGCGGAAGCATTACTGCAGGTATGGCCATCTACGACACG
ATGCAATTTATTAAGCCTGACGTTCAAACGATTTGTATCGGAATGGCTGCTTCCATGGGTGCTTTCTTATTGGCAGCCGG
CACGAAAGGAAAGCGTTATGCCCTTCCAAACAGTGAAGTGATGATCCATCAGCCGCTTGGCGGTGCCCAAGGCCAGGCAA
CAGAAATTGAGATCGCCGCAAAACGTATCTTGTTCCTTCGTGACAAGTTAAATCAAATCCTTTCAGAACGTACTGGACAG
CCGGTTGAAGTCATCGCAAAAGATACTGACCGCGATAACTTTATGACTGCCCAGCAGGCGCTCGAATATGGGTTAGTTGA
CCACATCATTACAAGAAATTCTCTTGAGGATAAGAGTAAAAAATAA

Domains

Predicted by InterproScan.

Clp protease (CLP_protease)

(13-192)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I3DU99

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  clpP Bacillus subtilis subsp. subtilis str. 168

85.354

98.507

0.841
  clpP Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

68.586

95.025

0.652
  clpP Streptococcus thermophilus LMG 18311

60.204

97.512

0.587
  clpP Streptococcus thermophilus LMD-9

60.204

97.512

0.587
  clpP Lactococcus lactis subsp. cremoris KW2

59.694

97.512

0.582
  clpP Streptococcus pneumoniae D39

58.462

97.015

0.567
  clpP Streptococcus pneumoniae Rx1

58.462

97.015

0.567
  clpP Streptococcus pneumoniae R6

58.462

97.015

0.567
  clpP Streptococcus pneumoniae TIGR4

58.462

97.015

0.567
  clpP Lactococcus lactis subsp. lactis strain DGCC12653

57.653

97.512

0.562
  clpP Streptococcus mutans UA159

56.345

98.01

0.552
  clpP Streptococcus pyogenes JRS4

56.122

97.512

0.547
  clpP Streptococcus pyogenes MGAS315

56.122

97.512

0.547

Multiple sequence alignment