Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   RK70_RS02400 Genome accession   NZ_CP026080
Coordinates   436763..437449 (+) Length   228 a.a.
NCBI ID   WP_001792568.1    Uniprot ID   A0A133PXY3
Organism   Staphylococcus aureus strain FDAARGOS_12     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 431763..442449
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RK70_RS02365 (RK70_002365) yqeH 432290..433390 (+) 1101 WP_001280141.1 ribosome biogenesis GTPase YqeH -
  RK70_RS02370 (RK70_002370) aroE 433404..434210 (+) 807 WP_000666761.1 shikimate dehydrogenase -
  RK70_RS02375 (RK70_002375) yhbY 434214..434504 (+) 291 WP_000955231.1 ribosome assembly RNA-binding protein YhbY -
  RK70_RS02380 (RK70_002380) nadD 434507..435076 (+) 570 WP_000725162.1 nicotinate (nicotinamide) nucleotide adenylyltransferase -
  RK70_RS02385 (RK70_002385) yqeK 435066..435650 (+) 585 WP_001019324.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  RK70_RS02390 (RK70_002390) rsfS 435651..436004 (+) 354 WP_001088022.1 ribosome silencing factor -
  RK70_RS02395 (RK70_002395) - 436007..436723 (+) 717 WP_000084829.1 class I SAM-dependent methyltransferase -
  RK70_RS02400 (RK70_002400) comEA 436763..437449 (+) 687 WP_001792568.1 ComEA family DNA-binding protein Machinery gene
  RK70_RS02405 (RK70_002405) - 437541..438002 (+) 462 WP_000439693.1 ComE operon protein 2 -
  RK70_RS02410 (RK70_002410) comEC 438007..440208 (+) 2202 WP_001805151.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  RK70_RS02415 (RK70_002415) holA 440265..441239 (+) 975 WP_001282562.1 DNA polymerase III subunit delta -
  RK70_RS02420 (RK70_002420) rpsT 441284..441535 (-) 252 WP_001274017.1 30S ribosomal protein S20 -

Sequence

Protein


Download         Length: 228 a.a.        Molecular weight: 25863.49 Da        Isoelectric Point: 9.3305

>NTDB_id=266843 RK70_RS02400 WP_001792568.1 436763..437449(+) (comEA) [Staphylococcus aureus strain FDAARGOS_12]
MVLLYQFLLRYKDFLTQWKLYILSAVVLIMVLIGFIFWRQDDYTSRNFENKDTALKQSTSENSSLSKVEDVQVKDGDNSK
NKGPVYVDVKGAVKHPNVYKMTSKDRVVDLLDKAQLLEDADVSQINLSEKLTDQKMIFIPHKGQKNVEPQIEVNSVHEKN
GNTNNTKVNLNTASVSELMSVPGVGQAKANAIVEYRNQQGAFQEIDDLKKVKGFGSKTFDKLKSYFTT

Nucleotide


Download         Length: 687 bp        

>NTDB_id=266843 RK70_RS02400 WP_001792568.1 436763..437449(+) (comEA) [Staphylococcus aureus strain FDAARGOS_12]
GTGGTTTTATTGTATCAATTTTTATTACGCTATAAAGATTTTTTAACTCAATGGAAGTTATATATTCTAAGTGCTGTTGT
TTTAATTATGGTATTAATTGGTTTTATATTCTGGAGACAAGATGATTATACTTCAAGAAATTTTGAAAATAAAGATACTG
CTCTGAAACAAAGCACTAGTGAAAATAGTAGTTTGTCCAAAGTAGAAGATGTCCAGGTCAAAGATGGAGATAATTCCAAA
AATAAGGGTCCTGTATATGTCGATGTAAAAGGTGCTGTTAAACATCCTAATGTTTATAAAATGACATCTAAGGATAGAGT
AGTTGATTTACTTGATAAAGCACAATTATTGGAGGATGCAGATGTAAGTCAAATTAATTTGTCTGAAAAATTAACAGATC
AAAAAATGATTTTCATACCTCATAAAGGACAAAAGAATGTTGAACCACAAATTGAAGTAAACAGTGTGCACGAAAAAAAT
GGGAACACAAATAATACTAAAGTAAATTTAAATACGGCATCTGTATCAGAATTGATGTCTGTTCCTGGAGTTGGGCAAGC
TAAAGCTAATGCAATTGTTGAATATCGCAACCAACAAGGTGCATTTCAAGAAATTGACGATTTGAAAAAAGTAAAAGGTT
TTGGAAGTAAAACTTTTGATAAACTGAAATCTTATTTCACGACATAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(164-227)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A133PXY3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Staphylococcus aureus MW2

98.238

99.561

0.978
  comEA Staphylococcus aureus N315

97.357

99.561

0.969

Multiple sequence alignment