Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   MLG_RS10550 Genome accession   NC_008340
Coordinates   2356460..2358196 (+) Length   578 a.a.
NCBI ID   WP_011629815.1    Uniprot ID   Q0A6W6
Organism   Alkalilimnicola ehrlichii MLHE-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2351460..2363196
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MLG_RS10525 (Mlg_2074) - 2352387..2352815 (+) 429 WP_011629810.1 HPP family protein -
  MLG_RS10530 (Mlg_2075) - 2352829..2353662 (+) 834 WP_011629811.1 motility protein A -
  MLG_RS10535 (Mlg_2076) - 2353652..2354407 (+) 756 WP_011629812.1 OmpA family protein -
  MLG_RS10540 (Mlg_2077) - 2354486..2355634 (+) 1149 WP_011629813.1 diguanylate cyclase -
  MLG_RS10545 (Mlg_2078) - 2355631..2356311 (+) 681 WP_011629814.1 rhomboid family intramembrane serine protease -
  MLG_RS10550 (Mlg_2079) pilB 2356460..2358196 (+) 1737 WP_011629815.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MLG_RS10555 (Mlg_2080) pilC 2358221..2359474 (+) 1254 WP_011629816.1 type II secretion system F family protein Machinery gene
  MLG_RS10560 (Mlg_2081) - 2359476..2360357 (+) 882 WP_011629817.1 A24 family peptidase -
  MLG_RS10565 (Mlg_2082) coaE 2360357..2361007 (+) 651 WP_011629818.1 dephospho-CoA kinase -
  MLG_RS10570 (Mlg_2083) zapD 2360994..2361851 (+) 858 WP_011629819.1 cell division protein ZapD -
  MLG_RS10575 (Mlg_2084) - 2361885..2362844 (-) 960 WP_011629820.1 Nudix family hydrolase -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 63459.82 Da        Isoelectric Point: 5.9905

>NTDB_id=26295 MLG_RS10550 WP_011629815.1 2356460..2358196(+) (pilB) [Alkalilimnicola ehrlichii MLHE-1]
MVTANPSVRLSGLAGRLVREGLLDEATARKHTEQAVKQRRPLISHLVEAKVLPGHEVIQQAAIEFGIPALDLAAVEPDIN
AVKLVSEKLIRTHQALPLFRRGKRLFLGVVDPTNLDALDEIKFHTGLTTEAILVEPDKLTQVMERALDAAAGGDAALKEL
DLDEDLEHLSVGGDEPREEREVGIAGEGEKDDAPVVRFVNKLLLDAIRKGASDIHFEPFEKEYRVRFRQDGILYEVSKPP
VNLGGRLAARLKVMSRMDIAEKRVPQDGRIKMNISRNRAIDFRVSTCPTLFGEKVVLRILDPSSAQMGIDSLGYEPEQKQ
RYLEALSKPYGMILVTGPTGSGKTVSLYTGLHILNTPDRNISTAEDPSEINMPGVNQVNINPKAGLTFANTLRAFLRQDP
DVIMVGEIRDLETAEIAIKAAQTGHLVLSTLHTNDAPQTLTRLANMGVPAYNIASSVTLIIAQRLARRLCKHCKVPEEVP
RETLLEEGFTEADLEAGVTVYAPQGCEHCTEGYKGRVGIYQVMPVSDAMGRLIMEGGNAMQLADQAAREGIDDLRRSGLR
KVIQGMTSLQEVNRVTKD

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=26295 MLG_RS10550 WP_011629815.1 2356460..2358196(+) (pilB) [Alkalilimnicola ehrlichii MLHE-1]
ATGGTCACTGCGAATCCCAGCGTCAGGCTGAGCGGTCTGGCGGGACGCCTGGTCCGCGAGGGCCTGCTTGATGAAGCGAC
GGCCCGAAAGCACACCGAGCAGGCCGTGAAACAGCGGCGCCCACTGATCAGCCATCTGGTCGAGGCCAAGGTGCTGCCGG
GCCATGAGGTCATCCAGCAGGCTGCCATCGAGTTCGGCATCCCGGCACTGGACCTGGCTGCCGTCGAGCCCGACATCAAT
GCCGTCAAGCTGGTTTCGGAGAAGCTGATCCGGACGCACCAGGCGCTGCCCCTGTTCCGCCGTGGCAAGCGGTTGTTCCT
GGGCGTGGTCGACCCCACCAACCTCGATGCCCTGGACGAGATCAAGTTCCACACGGGGCTGACCACCGAGGCCATCCTGG
TGGAGCCGGACAAGCTCACCCAGGTGATGGAGCGGGCGCTGGATGCGGCGGCCGGGGGCGATGCCGCGCTCAAGGAACTG
GACCTGGACGAGGACCTGGAGCACCTCTCGGTTGGCGGCGATGAGCCCCGGGAAGAGCGGGAGGTCGGCATCGCCGGCGA
GGGCGAGAAGGACGACGCCCCGGTGGTACGATTCGTCAACAAGCTCCTGCTCGACGCCATCCGCAAGGGCGCCTCGGACA
TCCACTTCGAACCCTTCGAAAAGGAGTACCGGGTCCGTTTCCGCCAGGACGGCATCCTCTACGAGGTCTCCAAACCGCCG
GTGAACCTGGGTGGACGCCTGGCCGCGCGCCTGAAGGTGATGTCGCGCATGGACATCGCGGAAAAGCGCGTACCCCAGGA
TGGGCGTATCAAGATGAACATCTCCCGCAACCGGGCCATCGACTTCCGGGTCAGTACCTGCCCCACGCTTTTCGGCGAAA
AGGTGGTGCTGCGTATCCTCGACCCCAGCAGTGCCCAGATGGGGATCGACTCCCTGGGCTACGAGCCGGAGCAGAAACAG
CGCTACCTGGAGGCACTGAGCAAGCCCTACGGGATGATCCTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGTGTCGCT
GTACACCGGCCTCCACATCCTCAACACCCCGGACCGGAACATCTCCACCGCCGAGGACCCCTCCGAGATCAACATGCCCG
GGGTCAACCAGGTCAACATCAACCCCAAGGCCGGGCTCACCTTTGCCAATACCCTGCGCGCCTTCCTGCGCCAGGATCCG
GACGTCATCATGGTGGGTGAGATCCGTGATCTGGAAACTGCCGAGATCGCCATCAAGGCGGCCCAGACCGGGCACCTGGT
GCTCTCCACCCTGCACACCAATGACGCCCCGCAAACCCTGACCCGCCTGGCCAACATGGGTGTGCCCGCCTACAACATCG
CCTCCTCGGTGACCCTGATCATCGCCCAACGGCTAGCCCGCCGGCTCTGCAAGCACTGCAAGGTGCCGGAGGAGGTCCCC
CGCGAGACCCTGCTGGAGGAGGGGTTCACCGAAGCGGATCTGGAGGCCGGCGTCACGGTCTACGCACCCCAGGGCTGCGA
GCACTGCACCGAGGGCTACAAGGGCCGGGTGGGTATCTACCAGGTGATGCCCGTCTCCGACGCCATGGGCCGCCTGATCA
TGGAGGGCGGCAACGCCATGCAGTTGGCCGACCAGGCGGCCAGAGAGGGGATTGATGACCTGCGCCGCTCCGGTCTGCGC
AAGGTCATTCAGGGGATGACCAGCCTGCAGGAAGTCAACCGGGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-150)

Type II/IV secretion system protein (T2SSE)

(201-468)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q0A6W6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

55.517

100

0.557
  pilB Acinetobacter baumannii D1279779

55.095

100

0.552
  pilB Acinetobacter baylyi ADP1

54.816

98.789

0.542
  pilB Vibrio campbellii strain DS40M4

49.385

98.443

0.486
  pilB Vibrio cholerae strain A1552

52.731

91.869

0.484
  pilB Vibrio parahaemolyticus RIMD 2210633

49.293

97.924

0.483
  pilF Neisseria gonorrhoeae MS11

52.811

86.159

0.455
  pilF Thermus thermophilus HB27

41.423

94.81

0.393

Multiple sequence alignment