Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   MLG_RS10555 Genome accession   NC_008340
Coordinates   2358221..2359474 (+) Length   417 a.a.
NCBI ID   WP_011629816.1    Uniprot ID   Q0A6W5
Organism   Alkalilimnicola ehrlichii MLHE-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2353221..2364474
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MLG_RS10535 (Mlg_2076) - 2353652..2354407 (+) 756 WP_011629812.1 OmpA family protein -
  MLG_RS10540 (Mlg_2077) - 2354486..2355634 (+) 1149 WP_011629813.1 diguanylate cyclase -
  MLG_RS10545 (Mlg_2078) - 2355631..2356311 (+) 681 WP_011629814.1 rhomboid family intramembrane serine protease -
  MLG_RS10550 (Mlg_2079) pilB 2356460..2358196 (+) 1737 WP_011629815.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MLG_RS10555 (Mlg_2080) pilC 2358221..2359474 (+) 1254 WP_011629816.1 type II secretion system F family protein Machinery gene
  MLG_RS10560 (Mlg_2081) - 2359476..2360357 (+) 882 WP_011629817.1 A24 family peptidase -
  MLG_RS10565 (Mlg_2082) coaE 2360357..2361007 (+) 651 WP_011629818.1 dephospho-CoA kinase -
  MLG_RS10570 (Mlg_2083) zapD 2360994..2361851 (+) 858 WP_011629819.1 cell division protein ZapD -
  MLG_RS10575 (Mlg_2084) - 2361885..2362844 (-) 960 WP_011629820.1 Nudix family hydrolase -
  MLG_RS10580 (Mlg_2085) argJ 2362849..2364045 (-) 1197 WP_011629821.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -

Sequence

Protein


Download         Length: 417 a.a.        Molecular weight: 45366.16 Da        Isoelectric Point: 10.1177

>NTDB_id=26296 MLG_RS10555 WP_011629816.1 2358221..2359474(+) (pilC) [Alkalilimnicola ehrlichii MLHE-1]
MAVATAKKASRKKPRQQPVFNWEGTDKRGAKVKGSMHSENALALKAELRRQGIIPSKVRKRSALEDLLSGGNKKIKPADI
AYFSRQLATMLQSGVPLVQALDIVGKGDEHAGMRQLVAEIKNDVESGTALHTALQKHPRYFDDLFVSLVAAGESAGVLDT
LLDKIATYKEKTESIKGKIKKALFYPTAVIVVAIVVTAILLIWVVPQFESLFRGFGADLPLFTQMVINLSDFMQSYWFIM
LAAAIGLGWGFSTAKRRSKAFSRSVDRFSLKIPAIGNILYKASVARFARTLATMFAAGVPLVEGLRSVASATGNYVFESA
VLQIREQVAAGQQLQISMRLSNLFPNMAIQMVAIGEESGSLDSMLAKVADYYEEEVDNAIDSLSSLLEPMIMAILGILVG
GLVIAMYLPIFQMGAAI

Nucleotide


Download         Length: 1254 bp        

>NTDB_id=26296 MLG_RS10555 WP_011629816.1 2358221..2359474(+) (pilC) [Alkalilimnicola ehrlichii MLHE-1]
ATGGCCGTCGCCACCGCAAAGAAAGCCAGTCGCAAGAAGCCCAGGCAGCAACCCGTGTTCAACTGGGAGGGCACCGACAA
GCGCGGCGCCAAGGTCAAGGGCAGCATGCACTCGGAGAATGCCCTGGCGCTGAAGGCCGAGCTGCGCCGCCAGGGCATCA
TCCCCTCCAAGGTGCGCAAGCGCTCCGCGCTGGAGGACCTGCTCAGCGGCGGCAACAAGAAGATCAAGCCGGCGGACATC
GCCTATTTCAGCCGCCAGCTCGCCACCATGCTGCAGTCCGGCGTGCCGCTGGTTCAGGCCCTGGATATCGTCGGCAAGGG
GGACGAGCACGCCGGCATGCGCCAGCTCGTGGCGGAGATCAAGAACGATGTGGAGTCGGGCACGGCCCTGCACACCGCCC
TACAGAAGCACCCGCGCTATTTCGATGACCTGTTCGTCAGTCTGGTGGCGGCCGGGGAGTCCGCGGGGGTGTTGGACACC
CTGCTGGACAAGATCGCCACCTACAAGGAAAAGACCGAGTCGATCAAGGGCAAGATCAAGAAGGCCCTGTTCTACCCCAC
GGCGGTGATCGTGGTGGCCATCGTGGTCACCGCCATCCTGCTGATCTGGGTCGTGCCGCAGTTCGAGTCGCTGTTCCGCG
GCTTCGGTGCCGACCTGCCGTTGTTCACCCAGATGGTGATCAACCTGTCGGACTTCATGCAGAGCTACTGGTTCATCATG
CTGGCCGCGGCCATCGGGCTGGGCTGGGGGTTCAGCACCGCCAAGCGACGATCGAAGGCCTTCTCACGCAGCGTGGACCG
GTTTTCGCTGAAGATCCCTGCCATCGGCAACATCCTTTACAAGGCCTCGGTGGCCCGCTTCGCCCGTACCCTCGCCACCA
TGTTCGCCGCCGGGGTGCCCCTGGTGGAGGGGCTGCGCTCGGTGGCCAGTGCCACCGGCAACTATGTGTTCGAGTCAGCG
GTGCTGCAGATTCGCGAGCAGGTGGCCGCCGGCCAGCAGCTGCAGATCTCCATGCGACTGTCCAATCTCTTCCCCAATAT
GGCCATCCAAATGGTGGCCATCGGCGAGGAGTCCGGCTCGTTGGACAGCATGCTCGCCAAGGTGGCCGACTACTACGAGG
AGGAGGTGGACAACGCCATCGATAGCCTCAGCAGCCTGCTGGAGCCGATGATCATGGCGATCCTCGGCATCCTGGTGGGC
GGACTGGTCATCGCCATGTATCTGCCCATCTTCCAGATGGGCGCCGCCATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(287-409)

Type II secretion system (T2SS), protein F (T2SSF)

(83-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q0A6W5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Acinetobacter baylyi ADP1

57.178

98.561

0.564
  pilC Legionella pneumophila strain ERS1305867

59.045

95.444

0.564
  pilC Pseudomonas stutzeri DSM 10701

57.143

97.362

0.556
  pilC Acinetobacter baumannii D1279779

56.618

97.842

0.554
  pilC Vibrio cholerae strain A1552

43.842

97.362

0.427
  pilG Neisseria gonorrhoeae MS11

42.963

97.122

0.417
  pilG Neisseria meningitidis 44/76-A

42.716

97.122

0.415
  pilC Vibrio campbellii strain DS40M4

40.609

94.484

0.384
  pilC Thermus thermophilus HB27

36.829

98.321

0.362

Multiple sequence alignment