Detailed information    

experimental Experimentally validated

Overview

Name   xerS   Type   Machinery gene
Locus tag   SPR_RS05225 Genome accession   NC_003098
Coordinates   1040078..1041148 (+) Length   356 a.a.
NCBI ID   WP_000817882.1    Uniprot ID   Q7ZAK7
Organism   Streptococcus pneumoniae R6     
Function   homologous recombination   
Homologous recombination

Function

A ∼1.45-fold drop in transformation was observed with total chromosomal DNA in recFOR mutants. Transformation with chromosomal DNA stimulated unexpectedly high frequency (>30% of cells) formation of chromosome dimers as an intermediate in the generation of tandem duplications, and that RecFOR were crucial for dimer resolution. The site-specific recombinase XerS was also crucial for dimer resolution.


Genomic Context

Location: 1035078..1046148
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SPR_RS11215 - 1035949..1036080 (+) 132 WP_079117188.1 LPXTG cell wall anchor domain-containing protein -
  SPR_RS10765 - 1036044..1036229 (-) 186 WP_075825865.1 hypothetical protein -
  SPR_RS05210 (spr1043) ylqF 1036311..1037162 (+) 852 WP_000201314.1 ribosome biogenesis GTPase YlqF -
  SPR_RS05215 (spr1044) - 1037149..1037928 (+) 780 WP_000201141.1 ribonuclease HII -
  SPR_RS05220 (spr1045) - 1037944..1039494 (+) 1551 WP_000392544.1 ClC family H(+)/Cl(-) exchange transporter -
  SPR_RS05225 (spr1046) xerS 1040078..1041148 (+) 1071 WP_000817882.1 tyrosine recombinase XerS Machinery gene
  SPR_RS05230 (spr1047) - 1041221..1042210 (-) 990 WP_000873993.1 lipoate--protein ligase -
  SPR_RS05235 (spr1048) lpdA 1042274..1043977 (-) 1704 WP_001162912.1 dihydrolipoyl dehydrogenase -
  SPR_RS05240 (spr1049) - 1044023..1045066 (-) 1044 WP_000752705.1 dihydrolipoamide acetyltransferase -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 41171.21 Da        Isoelectric Point: 9.6220

>NTDB_id=653 SPR_RS05225 WP_000817882.1 1040078..1041148(+) (xerS) [Streptococcus pneumoniae R6]
MKREILLERIDKLKQLMPWYVLEYYQSKLAVPYSFTTLYEYLKEYDRFFSWVLESGISNADKISDIPLSVLENMSKKDME
SFILYLRERPLLNANTTKQGVSQTTINRTLSALSSLYKYLTEEVENDQGEPYFYRNVMKKVSTKKKKETLAARAENIKQK
LFLGDETEGFLTYIDQEHPQQLSNRALSSFNKNKERDLAIIALLLASGVRLSEAVNLDLRDLNLKMMVIDVTRKGGKRDS
VNVAAFAKPYLENYLAIRNQRYKTEKTDTALFLTLYRGVPNRIDASSVEKMVAKYSEDFKVRVTPHKLRHTLATRLYDAT
KSQVLVSHQLGHASTQVTDLYTHIVNDEQKNALDSL

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=653 SPR_RS05225 WP_000817882.1 1040078..1041148(+) (xerS) [Streptococcus pneumoniae R6]
ATGAAACGTGAGATTTTACTGGAACGAATCGACAAACTAAAACAACTCATGCCCTGGTATGTTCTGGAATACTACCAATC
TAAACTGGCTGTGCCCTACAGTTTTACAACCCTGTACGAATACCTTAAGGAATATGACCGATTTTTCAGCTGGGTTTTGG
AGTCTGGTATTTCAAACGCTGATAAAATATCCGATATTCCTTTATCAGTTTTGGAAAATATGTCTAAGAAAGACATGGAA
TCCTTTATCCTTTATCTACGTGAACGTCCCTTGCTGAATGCTAATACAACAAAACAAGGTGTTTCACAGACAACTATCAA
TCGAACCTTATCAGCACTTTCTAGTCTTTACAAGTATCTAACCGAGGAGGTTGAAAACGATCAGGGGGAACCTTATTTCT
ATCGTAATGTAATGAAAAAAGTTTCAACCAAGAAAAAGAAAGAAACCCTTGCTGCCAGAGCTGAAAATATCAAGCAAAAA
CTCTTTCTAGGTGATGAAACAGAAGGTTTTCTAACTTATATCGATCAAGAGCACCCACAACAGCTTTCAAATCGAGCTCT
CTCATCATTCAACAAAAATAAAGAACGAGATTTAGCCATTATTGCCCTTCTCTTGGCATCTGGTGTTCGCTTATCTGAAG
CTGTTAATCTAGATCTAAGAGATCTCAATCTAAAAATGATGGTTATTGATGTTACTCGAAAAGGTGGCAAACGTGACTCA
GTCAATGTCGCTGCTTTTGCTAAACCTTATTTAGAGAATTATCTGGCCATTCGGAATCAACGCTATAAAACGGAAAAAAC
AGATACAGCCCTTTTTTTAACTCTCTACAGAGGTGTTCCTAATCGTATCGATGCTTCTAGCGTTGAGAAAATGGTTGCTA
AATACTCAGAGGATTTTAAAGTACGTGTAACACCCCATAAACTGCGCCATACACTAGCAACTAGGCTCTATGATGCTACT
AAATCACAAGTTTTAGTCAGTCACCAACTAGGACATGCCAGCACACAAGTCACTGACCTCTATACCCATATTGTTAATGA
TGAACAAAAGAATGCTCTGGATAGTTTATGA

Domains

Predicted by InterProScan.

Phage integrase family (Phage_integrase)

(188-344)

Phage integrase, N-terminal SAM-like domain (Phage_int_SAM_1)

(71-123)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q7ZAK7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Calum Johnston et al. (2015) RecFOR is not required for pneumococcal transformation but together with XerS for resolution of chromosome dimers frequently formed in the process. PLoS Genetics 11(1):e1004934. [PMID: 25569614]