Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   C0J56_RS02500 Genome accession   NZ_CP025542
Coordinates   562312..564399 (+) Length   695 a.a.
NCBI ID   WP_134922516.1    Uniprot ID   -
Organism   Pseudomonas fluorescens strain 2P24     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 557312..569399
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C0J56_RS02480 (C0J56_02485) pilM 559524..560588 (+) 1065 WP_134922513.1 pilus assembly protein PilM Machinery gene
  C0J56_RS02485 (C0J56_02490) - 560588..561154 (+) 567 WP_134922514.1 PilN domain-containing protein -
  C0J56_RS02490 (C0J56_02495) pilO 561151..561774 (+) 624 WP_109752039.1 type 4a pilus biogenesis protein PilO -
  C0J56_RS02495 (C0J56_02500) - 561771..562298 (+) 528 WP_134922515.1 pilus assembly protein PilP -
  C0J56_RS02500 (C0J56_02505) pilQ 562312..564399 (+) 2088 WP_134922516.1 type IV pilus secretin PilQ Machinery gene
  C0J56_RS02505 (C0J56_02510) aroK 564404..564922 (+) 519 WP_003196983.1 shikimate kinase AroK -
  C0J56_RS02510 (C0J56_02515) aroB 565071..566171 (+) 1101 WP_134922517.1 3-dehydroquinate synthase -
  C0J56_RS02515 (C0J56_02520) - 566182..567771 (+) 1590 WP_134922518.1 AAA family ATPase -

Sequence

Protein


Download         Length: 695 a.a.        Molecular weight: 75081.17 Da        Isoelectric Point: 5.4067

>NTDB_id=262449 C0J56_RS02500 WP_134922516.1 562312..564399(+) (pilQ) [Pseudomonas fluorescens strain 2P24]
MNRFFSALGISLWIALLSPMVHGASLKTLDVAALPGDRVELKLTFDGPPPTPRGYTTEQPARIALDLPGVTNQLASRNHD
LGGGNARSATVVEAGDRTRLIIGLTQLAPYDTRVEGNSLFVVVGKGAAPKSTAPKNTAQAPRPAVASAVPARASAPVGKA
IRGVDFQRGTQGEGNVVIDLSDPSIAPDIQEREGKIILNFARTQLPEPLRVRLDVKDFATPVQFVNASAGSDRATISIEP
SGTFDYSTYQTDNKLTVSVRPMTVDDLQKRNADRAAYSGEKLSLNFQDIEVRSVLQLIADFTNLNLVASDTVQGGITLRL
QNVPWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIADLAPLRRELLQVNYAKAADIAKLFQSVTSA
EAKADERGTITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNKGNWNSSG
VTTGDSTTIGTPGSTSTNQPFVDLGASGNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTE
IPYQEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGV
FSNTQSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2088 bp        

>NTDB_id=262449 C0J56_RS02500 WP_134922516.1 562312..564399(+) (pilQ) [Pseudomonas fluorescens strain 2P24]
ATGAACAGGTTTTTTTCAGCCCTCGGTATTTCGCTATGGATAGCGCTGTTGTCGCCGATGGTTCATGGGGCCAGTCTGAA
GACGCTGGATGTGGCGGCGTTGCCGGGGGACCGTGTCGAGTTGAAGCTCACCTTCGATGGACCGCCGCCGACTCCTCGGG
GCTACACCACCGAACAACCGGCCCGGATTGCCCTGGACCTGCCCGGGGTGACCAATCAACTGGCCAGCAGGAACCACGAC
CTGGGCGGGGGCAATGCACGCAGCGCCACCGTGGTCGAGGCCGGGGATCGCACCCGGTTGATCATCGGCCTTACCCAACT
GGCGCCGTATGACACCCGGGTCGAAGGTAACAGCCTGTTCGTGGTGGTCGGCAAGGGGGCCGCTCCGAAGAGCACTGCCC
CGAAGAACACCGCCCAGGCCCCGCGCCCGGCGGTTGCCAGCGCCGTGCCTGCGAGGGCCAGTGCGCCGGTGGGCAAGGCG
ATCCGTGGGGTGGACTTCCAGCGCGGGACCCAGGGCGAAGGCAACGTGGTGATCGACCTGTCTGATCCGTCCATCGCCCC
GGACATCCAGGAGCGTGAAGGCAAGATCATCCTCAATTTCGCCAGAACCCAATTGCCCGAGCCATTGCGGGTGCGCCTGG
ACGTCAAGGACTTCGCCACCCCGGTGCAGTTCGTCAATGCCAGCGCCGGCAGCGACCGGGCGACCATCAGCATCGAGCCC
AGCGGTACCTTCGACTACTCGACCTACCAGACCGACAACAAGCTCACCGTCAGCGTCCGGCCCATGACCGTCGACGACCT
GCAAAAACGCAACGCCGACCGCGCGGCCTACAGTGGCGAGAAGCTCTCGCTCAACTTCCAGGACATCGAGGTACGCTCGG
TCCTGCAATTGATCGCCGATTTCACCAACCTGAACCTGGTGGCCAGCGACACGGTGCAGGGCGGCATCACCCTGCGCTTG
CAGAACGTGCCTTGGGACCAGGCGCTGGATCTGGTGCTCAAGACCAAGGGGCTGGACAAGCGCAAGGTCGGCAACGTGCT
GCTAGTGGCTCCGGCCGATGAAATTGCGGCCCGCGAGCGCCAGGAGCTGGAGTCGCAGAAGCAGATTGCCGACCTGGCAC
CACTGCGGCGTGAGCTGTTGCAGGTCAACTACGCCAAGGCGGCGGACATCGCCAAGCTGTTCCAGTCAGTGACCAGTGCC
GAGGCTAAGGCCGACGAGCGCGGCACGATCACGGTGGATGAGCGAACCAACAACATCATCGCCTACCAGACCCAGGACCG
CCTCGACGAACTGCGCAGGATCGTGGCGCAACTGGATATTCCGGTGCGCCAGGTGATGATCGAGGCGCGGATCGTCGAGG
CCAACGTCGATTACGACAAAAGCCTGGGTGTGCGTTGGGGCGGTTCGATCCAGAACAAGGGCAACTGGAATTCCTCCGGG
GTCACCACCGGCGACTCCACCACCATTGGCACGCCGGGCAGTACCAGCACCAACCAGCCGTTTGTCGATCTGGGCGCGTC
GGGCAATACCTCCGGAATCGGCATTGCGTTCATCACCGACAACGTGCTGCTGGATCTGGAGCTGACGGCCATGGAGAAGA
CCGGCAACGGTGAAATCGTCTCCCAGCCCAAGGTGGTGACCTCCGACAAGGAGACGGCAAAAATCCTCAAGGGCACGGAA
ATCCCTTATCAGGAAGCCAGTTCCAGCGGTGCCACGTCGGTGTCGTTCAAGGAGGCTTCGCTGTCGCTGGAGGTGACTCC
GCAGATCACCCCGGACAATCGCATCATCATGGAGGTCAAGGTCACCAAGGATGAGCCGGACTACTTGAACAAGGTGCAGG
ATGTACCGCCAATCAAGAAAAACGAGGTCAACGCCAAGGTCTTGGTCAATGACGGCGAGACCATTGTGATTGGCGGGGTT
TTCTCAAATACTCAGAGCAAGGTTGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCG
TGATGTGGTCTCGGAGAAAAAATCCGAGCTGCTGGTGTTCCTCACTCCGCGTATCATGAACAACCAGGCGATTGCTGTGA
GTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(379-444)

AMIN domain (AMIN)

(38-122)

Bacterial type II and III secretion system protein (Secretin)

(530-686)

Secretin and TonB N terminus short domain (STN)

(305-351)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.905

100

0.751
  pilQ Acinetobacter baumannii strain A118

38.658

100

0.414
  pilQ Acinetobacter baumannii D1279779

38.658

100

0.414
  comQ Acinetobacter baylyi ADP1

41.379

100

0.414
  pilQ Legionella pneumophila strain Lp02

37.85

97.698

0.37
  pilQ Legionella pneumophila strain ERS1305867

37.85

97.698

0.37

Multiple sequence alignment