Detailed information NTG0262448

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: C0J56_RS02480
• Genome accession: NZ_CP025542
• Coordinates: 559524..560588 (+)
• Length: 354 a.a.
• NCBI ID: WP_134922513.1
• Uniprot ID: -
• Organism: Pseudomonas fluorescens strain 2P24
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 554524..565588

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
C0J56_RS02465 (C0J56_02470)	rpmE	554755..554979 (+)	225	WP_053117645.1	50S ribosomal protein L31	-
C0J56_RS02470 (C0J56_02475)	-	555304..556572 (+)	1269	WP_134922511.1	malic enzyme-like NAD(P)-binding protein	-
C0J56_RS02475 (C0J56_02480)	-	556871..559315 (-)	2445	WP_167659372.1	penicillin-binding protein 1A	-
C0J56_RS02480 (C0J56_02485)	pilM	559524..560588 (+)	1065	WP_134922513.1	pilus assembly protein PilM	Machinery gene
C0J56_RS02485 (C0J56_02490)	-	560588..561154 (+)	567	WP_134922514.1	PilN domain-containing protein	-
C0J56_RS02490 (C0J56_02495)	pilO	561151..561774 (+)	624	WP_109752039.1	type 4a pilus biogenesis protein PilO	-
C0J56_RS02495 (C0J56_02500)	-	561771..562298 (+)	528	WP_134922515.1	pilus assembly protein PilP	-
C0J56_RS02500 (C0J56_02505)	pilQ	562312..564399 (+)	2088	WP_134922516.1	type IV pilus secretin PilQ	Machinery gene
C0J56_RS02505 (C0J56_02510)	aroK	564404..564922 (+)	519	WP_003196983.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38037.61 Da
• Isoelectric Point: 4.9070

>NTDB_id=262448 C0J56_RS02480 WP_134922513.1 559524..560588(+) (pilM) [Pseudomonas fluorescens strain 2P24]
MLRLFNKKAHALLGIDISSTSVKLLELSRQGERYRVESYAVEPLPANAVVEKNIADLEGVGQALSRLLAKAKTPVRSVAV
AVAGSAVITKTIEMDAGLSDDDMENQLKIEADQYIPYPLDEVAIDFEVVGVSPRSAERVEVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERAFGLLATQLAASQERLTVAVIDIGATMTTLSVLHNGRIIYTREQLFGGRQLTEEIQRRYGLTP
EQAGLAKKQGGLPDDYLSEVLQPFREALVQQVSRSLQFFFASGQYSAVDHILLAGGTASVAGLDRLIEQRLGTPTQVANP
FTNMALGSKVNAGALASDAPALMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=262448 C0J56_RS02480 WP_134922513.1 559524..560588(+) (pilM) [Pseudomonas fluorescens strain 2P24]
GTGCTACGACTCTTCAATAAAAAAGCCCATGCGCTTCTGGGGATCGACATCAGTTCGACGTCGGTGAAGCTGCTCGAGCT
GAGCCGCCAGGGCGAGCGATACCGCGTCGAGTCCTATGCGGTCGAACCGTTGCCGGCCAACGCCGTGGTCGAGAAGAACA
TCGCCGATCTCGAAGGGGTGGGCCAGGCATTGTCGCGGCTGTTGGCCAAGGCCAAGACGCCCGTGCGTAGCGTGGCGGTG
GCCGTTGCGGGGTCGGCGGTGATCACCAAGACCATCGAGATGGACGCCGGGTTGTCCGACGATGACATGGAAAACCAGCT
CAAGATCGAGGCCGATCAATACATTCCCTATCCGCTGGATGAGGTGGCCATCGATTTTGAAGTGGTGGGCGTTTCGCCGC
GCAGTGCCGAGCGCGTCGAAGTGCTGCTGGCGGCCTGCCGCAAGGAAAACGTCGAGGTCCGCGAGGCTGCGTTGGCGCTC
GCCGGGCTGACGGCCCGGGTGGTCGATGTGGAGGCTTATGCGCTGGAGCGCGCTTTCGGCCTGCTTGCCACGCAATTGGC
CGCCTCCCAGGAGCGGCTGACCGTGGCGGTTATCGACATCGGCGCCACCATGACCACCCTCAGCGTGCTGCACAACGGGC
GCATCATCTATACCCGCGAACAATTGTTCGGCGGCCGCCAGTTGACCGAGGAGATTCAGCGTCGCTATGGCTTGACCCCC
GAGCAGGCCGGCTTGGCAAAGAAGCAGGGAGGGCTGCCGGACGACTACCTGAGCGAAGTGTTGCAGCCCTTTCGCGAGGC
CCTGGTTCAGCAGGTTTCACGATCCCTGCAGTTCTTCTTCGCCTCGGGCCAGTACAGTGCGGTCGACCATATCCTGCTGG
CCGGGGGCACGGCTTCGGTCGCCGGCCTGGATCGGCTGATCGAGCAGCGCCTGGGTACGCCGACCCAAGTGGCCAACCCG
TTTACCAACATGGCCCTGGGCAGCAAGGTCAATGCCGGGGCGCTGGCCAGTGATGCGCCGGCGCTGATGATTGCCTGCGG
GCTGGCCCTCAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	55.65	100	0.557
comM	Acinetobacter nosocomialis M2	55.65	100	0.557
comM	Acinetobacter baylyi ADP1	54.237	100	0.542
pilM	Legionella pneumophila strain ERS1305867	48.305	100	0.483