Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   CXB49_RS02130 Genome accession   NZ_CP025429
Coordinates   496779..498485 (+) Length   568 a.a.
NCBI ID   WP_101706875.1    Uniprot ID   A0A2H5DRM4
Organism   Chromobacterium sp. ATCC 53434     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 491779..503485
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CXB49_RS02110 (CXB49_02110) ffh 492381..493736 (-) 1356 WP_101706871.1 signal recognition particle protein -
  CXB49_RS02115 (CXB49_02115) - 493848..494669 (+) 822 WP_101706872.1 inner membrane protein YpjD -
  CXB49_RS02120 (CXB49_02120) comE 494723..495046 (+) 324 WP_101706873.1 ComEA family DNA-binding protein Machinery gene
  CXB49_RS02125 (CXB49_02125) - 495439..496563 (+) 1125 WP_101706874.1 porin -
  CXB49_RS02130 (CXB49_02130) pilF 496779..498485 (+) 1707 WP_101706875.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CXB49_RS02135 (CXB49_02135) pilC 498526..499749 (+) 1224 WP_101706876.1 type II secretion system F family protein Machinery gene
  CXB49_RS02140 (CXB49_02140) - 499749..500669 (+) 921 WP_101706877.1 A24 family peptidase -
  CXB49_RS02145 (CXB49_02145) coaE 500654..501268 (+) 615 WP_101706878.1 dephospho-CoA kinase -
  CXB49_RS02150 (CXB49_02150) zapD 501305..502063 (+) 759 WP_101706879.1 cell division protein ZapD -
  CXB49_RS02155 (CXB49_02155) yacG 502067..502258 (+) 192 WP_101706880.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62255.63 Da        Isoelectric Point: 5.5715

>NTDB_id=261263 CXB49_RS02130 WP_101706875.1 496779..498485(+) (pilF) [Chromobacterium sp. ATCC 53434]
MEASAGISGLGRALVQHNMLLQQDADAIQRQATASHISFVEQLILSKKMSARDVAVFASRIFGYPLLDMGTLDTEQLPKG
LVDEDLIGSRRLLPLLKRGNKLYIGTSDPTQTSAYHAITFKTGLTVEIVVVEDDKLSKTLGRYQDNSTSALKELENEDFG
DLEFADPDAQIDSGPQVEVDDAPVVKFIHKMLLDAIGGGASDIHFEPYEKYYRIRYRVDGMLKEVAQPPLLLKEKIASRI
KVISKLDISEKRVPQDGRMKLVISKSRAIDFRVSTLPTLYGEKIVMRILDSSAASLDIEQLGFEPEQKKLLLNAIARPYG
MVLVTGPTGSGKTVSLYTCLSILNKSDTNISTAEDPVEINLPGINQVNVNERAGLSFASALKAFLRQDPDVIMVGEIRDF
ETADIAIKAAQTGHMVFSTLHTNNAPATLTRMLNMGVAPFNIASSVLLIMAQRLARKLCSHCKQPVDIPEEALLRAGFAK
EQLDGSWQAYGPVGCEECRNTGYRGRTGLYEVMPISDAMTRLIMKNGTAVDIADLAKEEGMVDLRHAGLMKVRQGVTSLA
EIEAVTND

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=261263 CXB49_RS02130 WP_101706875.1 496779..498485(+) (pilF) [Chromobacterium sp. ATCC 53434]
ATGGAAGCTTCGGCAGGAATTTCCGGCCTGGGAAGGGCCCTGGTTCAGCACAATATGCTGTTGCAGCAGGACGCCGACGC
GATTCAGCGCCAGGCGACCGCGTCCCATATCAGCTTCGTCGAGCAGCTGATTCTCAGCAAGAAGATGAGCGCCCGCGACG
TCGCGGTCTTCGCTTCGCGCATTTTCGGCTACCCCTTGCTGGACATGGGGACGCTGGATACCGAGCAACTGCCCAAGGGC
CTGGTCGACGAGGATCTGATCGGCAGCCGCCGGCTGTTGCCGCTGCTGAAGCGCGGCAACAAGCTGTATATCGGCACGTC
GGATCCGACCCAGACCTCGGCCTATCACGCGATCACCTTCAAGACCGGCCTGACGGTGGAGATCGTCGTGGTCGAGGACG
ACAAGCTGTCGAAAACGCTGGGGCGCTATCAGGACAATTCCACGTCGGCGCTGAAGGAGCTGGAGAACGAGGATTTCGGC
GATCTGGAATTCGCCGATCCGGACGCGCAGATAGATTCCGGCCCGCAGGTCGAGGTCGACGACGCGCCGGTGGTCAAATT
CATCCACAAGATGCTGCTGGACGCGATAGGCGGCGGGGCCTCCGACATCCACTTCGAGCCGTACGAAAAGTATTACCGCA
TCCGCTACCGCGTCGACGGCATGTTGAAGGAGGTGGCGCAGCCGCCCTTGCTGCTGAAGGAAAAGATCGCCTCCCGCATC
AAGGTGATCTCCAAGCTCGACATCTCGGAAAAACGCGTGCCGCAGGACGGCCGGATGAAGCTGGTGATTTCGAAGAGCCG
GGCGATAGATTTTCGCGTCAGCACGCTGCCGACCTTGTACGGCGAGAAGATCGTGATGCGGATACTGGATTCGTCGGCGG
CGTCGCTGGACATCGAGCAGCTCGGCTTCGAGCCGGAGCAGAAGAAATTGCTGCTGAACGCGATCGCCCGGCCGTACGGC
ATGGTGCTGGTCACCGGGCCGACCGGCAGCGGCAAGACGGTGTCGCTGTACACCTGTCTGAGCATTTTGAACAAGTCCGA
CACCAATATCTCCACCGCCGAGGATCCGGTGGAAATCAATTTGCCCGGCATCAATCAGGTCAATGTCAACGAGCGGGCCG
GCCTCAGCTTCGCTTCGGCGCTGAAGGCTTTTCTGCGGCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGACTTC
GAAACCGCCGACATCGCGATCAAGGCGGCGCAGACCGGCCACATGGTGTTTTCGACACTGCATACCAATAACGCGCCGGC
CACGCTGACGCGGATGCTCAATATGGGGGTGGCGCCGTTCAATATCGCCAGCTCGGTATTGTTGATCATGGCGCAGCGGT
TGGCGCGCAAACTGTGCAGCCACTGCAAGCAGCCGGTGGACATTCCGGAAGAGGCGCTGCTGCGGGCCGGCTTCGCCAAG
GAGCAGCTGGACGGCAGCTGGCAGGCCTATGGTCCGGTCGGCTGCGAAGAATGCCGCAACACCGGTTACAGGGGGCGTAC
CGGCCTGTACGAGGTGATGCCGATCAGCGACGCGATGACGCGGCTGATCATGAAGAATGGCACCGCGGTCGACATCGCCG
ACCTGGCGAAGGAAGAGGGCATGGTCGATCTGCGCCACGCCGGCCTGATGAAGGTCAGGCAGGGTGTCACCTCGCTGGCG
GAAATCGAGGCGGTGACCAACGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-148)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2H5DRM4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

57.829

98.944

0.572
  pilB Acinetobacter baumannii D1279779

54.007

100

0.546
  pilB Legionella pneumophila strain ERS1305867

53.752

100

0.542
  pilB Acinetobacter baylyi ADP1

54.352

99.12

0.539
  pilB Vibrio cholerae strain A1552

49.734

99.12

0.493
  pilB Vibrio parahaemolyticus RIMD 2210633

48.399

98.944

0.479
  pilB Vibrio campbellii strain DS40M4

47.872

99.296

0.475
  pilF Thermus thermophilus HB27

37.655

99.12

0.373
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.49

95.599

0.368

Multiple sequence alignment