Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   SmaCSM2_RS16925 Genome accession   NZ_CP025298
Coordinates   3680480..3682210 (-) Length   576 a.a.
NCBI ID   WP_101765982.1    Uniprot ID   A0AAD0FN56
Organism   Stenotrophomonas maltophilia strain CSM2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3675480..3687210
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SmaCSM2_RS16910 (SmaCSM2_16910) - 3676701..3678314 (+) 1614 WP_087923443.1 ATP-binding protein -
  SmaCSM2_RS16915 (SmaCSM2_16915) pilR 3678375..3679748 (+) 1374 WP_101765981.1 sigma-54 dependent transcriptional regulator Regulator
  SmaCSM2_RS16920 (SmaCSM2_16920) - 3679963..3680370 (+) 408 WP_158298006.1 hypothetical protein -
  SmaCSM2_RS16925 (SmaCSM2_16925) pilB 3680480..3682210 (-) 1731 WP_101765982.1 type IV-A pilus assembly ATPase PilB Machinery gene
  SmaCSM2_RS16930 (SmaCSM2_16930) - 3682476..3682925 (-) 450 WP_080347436.1 pilin -
  SmaCSM2_RS16935 (SmaCSM2_16935) pilA 3683036..3683485 (-) 450 WP_049442650.1 pilin Machinery gene
  SmaCSM2_RS16940 (SmaCSM2_16940) pilC 3683840..3685099 (+) 1260 WP_049442651.1 type II secretion system F family protein Machinery gene
  SmaCSM2_RS16945 (SmaCSM2_16945) - 3685108..3685971 (+) 864 WP_005418626.1 A24 family peptidase -
  SmaCSM2_RS16950 (SmaCSM2_16950) coaE 3685983..3686594 (+) 612 WP_101765984.1 dephospho-CoA kinase -
  SmaCSM2_RS16955 (SmaCSM2_16955) - 3686655..3687041 (-) 387 WP_101765985.1 hypothetical protein -

Sequence

Protein


Download         Length: 576 a.a.        Molecular weight: 62335.27 Da        Isoelectric Point: 5.8733

>NTDB_id=259893 SmaCSM2_RS16925 WP_101765982.1 3680480..3682210(-) (pilB) [Stenotrophomonas maltophilia strain CSM2]
MNAVTTANLVGITGLARRLVQDGALDEASARDAMAKATVARQPLPTWFAQNKVVGAAQLAAANAVEFGMPLFDVSTFDAS
QNAMSLVSEELLRKHNVLPLFKRGGKLFVGTSNPTHSLDEIKFHTNLVVEPILVDEDQIRRTFEQWHASHDTLGDALGGD
DDGMGNLDVAAGDEDLGTGGDSGIDAKGDDTPVVKFVNKVLVDAIRKGASDIHFEPYEDDYRVRLRIDGLLKMVARAPVK
LNQRIAARLKVMAQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEPDQQKLF
LEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPDI
IMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRPSNLPEH
ALLAEGFTQAQLDAGIELYEAVGCDECTEGYKGRTGLYQVMPMTDEIATIVLAGGNALQIAEAAQQIGVNDLRQSALKKA
AAGVTSLAEINRVTKD

Nucleotide


Download         Length: 1731 bp        

>NTDB_id=259893 SmaCSM2_RS16925 WP_101765982.1 3680480..3682210(-) (pilB) [Stenotrophomonas maltophilia strain CSM2]
ATGAACGCCGTAACCACCGCCAATCTCGTCGGCATCACCGGCCTGGCTCGTCGCCTGGTCCAGGATGGTGCGCTGGACGA
GGCTTCCGCCCGCGACGCGATGGCCAAGGCCACCGTGGCCCGTCAGCCGCTGCCGACCTGGTTCGCGCAGAACAAGGTGG
TTGGGGCGGCCCAGCTGGCGGCCGCCAATGCGGTCGAGTTCGGCATGCCGCTGTTCGACGTGTCCACGTTCGACGCCAGC
CAGAACGCTATGAGCCTGGTCAGCGAGGAGCTGCTGCGCAAGCACAACGTGCTGCCGCTGTTCAAACGCGGCGGCAAACT
GTTCGTGGGCACCAGCAACCCGACCCATTCGCTGGACGAGATCAAGTTCCACACCAATCTGGTGGTGGAGCCGATCCTGG
TGGACGAGGACCAGATCCGCCGCACATTCGAGCAGTGGCATGCCAGCCACGACACGCTGGGCGACGCCCTGGGCGGTGAC
GACGATGGCATGGGCAACCTGGACGTTGCGGCCGGAGACGAGGATCTCGGCACCGGCGGCGACTCCGGTATCGATGCCAA
GGGCGATGACACCCCAGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGACGCGATCCGCAAGGGCGCCTCGGACATCCACT
TCGAGCCCTATGAAGATGATTACCGCGTGCGCCTGCGCATCGACGGCCTGCTGAAGATGGTCGCGCGCGCGCCAGTGAAG
CTGAACCAGCGCATCGCCGCACGCCTGAAGGTGATGGCGCAGCTGGATATCGCCGAAAAGCGCGTGCCGCAGGACGGGCG
CATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGCGTGAGCACGCTGCCGACCCTGTTCGGCGAGAAGGTGG
TGCTGCGTATCCTGGACGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGTTACGAGCCGGACCAGCAGAAGCTGTTC
CTGGAAGCCATCCACAAGCCCTACGGCATGGTGCTGGTGACCGGCCCGACCGGTTCGGGCAAGACGGTGTCGCTGTATAC
GGCGCTGGGCATCCTCAACGACGAGACCCGCAACATCTCCACCGCCGAAGACCCGGTGGAAATCCGCCTGCCCGGCGTCA
ACCAGGTGCAGCAGAACAACAAGCGCGGCATGACCTTCGCCGCAGCGTTGCGCTCGTTCCTGCGACAGGATCCGGACATC
ATCATGGTCGGCGAAATCCGCGACCTGGAGACGGCCGAGATCGCGATCAAGGCGGCGCAGACCGGCCACATGGTGCTGTC
CACGCTGCATACCAACGATGCACCGCAGACCATCGCGCGCCTGATGAACATGGGCATCGCGCCGTACAACATCACCAGTT
CGGTGACCCTGGTGATCGCCCAGCGCCTGGCGCGCCGGCTGTGCAACAACTGCAAGCGCCCGTCCAACCTGCCCGAGCAT
GCGCTGCTGGCCGAGGGCTTCACCCAGGCGCAGCTGGATGCCGGTATCGAACTGTACGAGGCAGTGGGCTGCGATGAGTG
CACCGAGGGCTACAAGGGCCGTACCGGTCTGTACCAGGTGATGCCGATGACCGATGAGATCGCCACGATTGTGCTGGCCG
GTGGCAATGCGTTGCAGATTGCCGAGGCCGCACAGCAGATCGGGGTGAACGATCTGCGCCAGTCGGCGTTGAAGAAGGCG
GCTGCGGGTGTCACCAGCCTGGCCGAGATCAATCGCGTTACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-150)

Type II/IV secretion system protein (T2SSE)

(199-466)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.623

100

0.554
  pilB Acinetobacter baumannii D1279779

54.922

100

0.552
  pilB Legionella pneumophila strain ERS1305867

51.67

98.785

0.51
  pilF Neisseria gonorrhoeae MS11

48.592

98.611

0.479
  pilB Vibrio cholerae strain A1552

50.647

93.924

0.476
  pilB Vibrio parahaemolyticus RIMD 2210633

44.618

100

0.446
  pilB Vibrio campbellii strain DS40M4

44.991

98.785

0.444

Multiple sequence alignment