Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA/cilB/dalA   Type   Machinery gene
Locus tag   CXP32_RS06265 Genome accession   NZ_CP025256
Coordinates   1199287..1200135 (-) Length   282 a.a.
NCBI ID   WP_000705306.1    Uniprot ID   A0A098Z7D2
Organism   Streptococcus pneumoniae strain Xen35     
Function   ssDNA binding; loading RecA onto ssDNA; competence shut-off (predicted from homology)   
DNA processing Competence regulation

Genomic Context

Location: 1194287..1205135
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CXP32_RS06255 (CXP32_06255) topA 1195088..1197187 (-) 2100 WP_000179649.1 type I DNA topoisomerase -
  CXP32_RS06260 (CXP32_06260) - 1197354..1198397 (-) 1044 WP_001812957.1 TIGR00341 family protein -
  CXP32_RS12590 - 1198981..1199103 (+) 123 WP_001814242.1 hypothetical protein -
  CXP32_RS06265 (CXP32_06265) dprA/cilB/dalA 1199287..1200135 (-) 849 WP_000705306.1 DNA-processing protein DprA Machinery gene
  CXP32_RS06270 (CXP32_06270) - 1200231..1200920 (-) 690 WP_000643971.1 sugar phosphate nucleotidyltransferase -
  CXP32_RS06275 (CXP32_06275) - 1200932..1201810 (-) 879 WP_000837617.1 DMT family transporter -
  CXP32_RS06280 (CXP32_06280) licA 1201800..1202669 (-) 870 WP_000411215.1 choline kinase LicA -
  CXP32_RS06285 (CXP32_06285) - 1202686..1203708 (-) 1023 WP_000609885.1 ribitol-5-phosphate dehydrogenase -
  CXP32_RS06290 (CXP32_06290) - 1203713..1204420 (-) 708 WP_000638508.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -

Sequence

Protein


Download         Length: 282 a.a.        Molecular weight: 31062.72 Da        Isoelectric Point: 5.4047

>NTDB_id=259463 CXP32_RS06265 WP_000705306.1 1199287..1200135(-) (dprA/cilB/dalA) [Streptococcus pneumoniae strain Xen35]
MKITNYEIYKLKKSGLTNQQILKVLEYGENVDQELLLGDIADISGCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDC
YPWDLSEIYDAPVLLFYKGNLDLLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
TIAVIGTGLDVFYPKANKRLQDYIGNDHLVLSEYGPGEQPLKFHFPARNRIIAGLCRGVIVAEAKMRSGSLITCERAMEE
GRDVFAIPGSILDGLSDGCHHLIQEGAKLVTSGQDVLAEFEF

Nucleotide


Download         Length: 849 bp        

>NTDB_id=259463 CXP32_RS06265 WP_000705306.1 1199287..1200135(-) (dprA/cilB/dalA) [Streptococcus pneumoniae strain Xen35]
ATGAAAATCACAAACTATGAAATCTATAAGTTAAAAAAATCAGGTTTGACCAATCAACAGATTTTGAAAGTGCTAGAATA
CGGTGAAAATGTTGATCAGGAGCTTTTGTTGGGTGATATTGCAGATATCTCAGGTTGCCGTAATCCAGCCGTTTTTATGG
AACGTTATTTTCAGATAGACGATGCGCATTTGTCGAAAGAGTTTCAAAAATTTCCATCTTTCTCTATTTTAGATGACTGT
TATCCTTGGGATTTGAGTGAAATATATGATGCGCCTGTACTTTTATTTTACAAGGGAAATCTTGACCTCCTGAAATTCCC
GAAGGTAGCGGTCGTGGGCAGTCGTGCTTGTAGCAAACAGGGAGCTAAGTCAGTTGAAAAAGTCATTCAAGGCTTGGAAA
ATGAACTGGTTATTGTCAGTGGTCTGGCCAAGGGCATTGACACAGCAGCTCATATGGCAGCTCTTCAGAATGGCGGAAAA
ACCATTGCAGTGATTGGAACAGGACTGGATGTGTTTTATCCTAAAGCCAATAAACGCTTGCAAGACTACATCGGCAATGA
CCATCTGGTTCTAAGTGAATATGGACCTGGTGAACAACCTCTGAAATTTCATTTTCCTGCCCGTAATCGCATCATTGCTG
GACTTTGTCGTGGTGTGATTGTAGCAGAGGCTAAGATGCGTTCAGGTAGTCTCATTACGTGTGAGCGAGCAATGGAAGAA
GGACGCGATGTCTTTGCTATTCCTGGTAGCATTTTAGATGGACTATCAGACGGTTGCCATCATTTGATTCAAGAAGGAGC
AAAATTGGTCACCAGTGGGCAAGATGTTCTTGCGGAATTTGAATTTTAA

Domains

Predicted by InterproScan.

DNA recombination-mediator protein A (DNA_processg_A)

(75-271)

DNA processing protein A sterile alpha motif domain (SAM_DprA)

(3-64)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A098Z7D2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA/cilB/dalA Streptococcus pneumoniae Rx1

100

100

1
  dprA/cilB/dalA Streptococcus pneumoniae D39

100

100

1
  dprA/cilB/dalA Streptococcus pneumoniae R6

100

100

1
  dprA/cilB/dalA Streptococcus pneumoniae TIGR4

100

100

1
  dprA/cilB/dalA Streptococcus mitis NCTC 12261

98.582

100

0.986
  dprA/cilB/dalA Streptococcus mitis SK321

97.872

100

0.979
  dprA Streptococcus mutans UA159

63.214

99.291

0.628
  dprA Lactococcus lactis subsp. cremoris KW2

55.714

99.291

0.553
  dprA Staphylococcus aureus N315

37.809

100

0.379
  dprA Haemophilus influenzae Rd KW20

38.202

94.681

0.362

Multiple sequence alignment