Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   CXB48_RS02960 Genome accession   NZ_CP025149
Coordinates   623319..625418 (+) Length   699 a.a.
NCBI ID   WP_041755270.1    Uniprot ID   -
Organism   Stutzerimonas stutzeri strain SGAir0442     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 618319..630418
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CXB48_RS02940 (CXB48_02935) comM 620482..621546 (+) 1065 WP_017244291.1 pilus assembly protein PilM Machinery gene
  CXB48_RS02945 (CXB48_02940) pilN 621546..622118 (+) 573 WP_014595705.1 type 4a pilus biogenesis protein PilN -
  CXB48_RS02950 (CXB48_02945) pilO 622115..622741 (+) 627 WP_095460896.1 type 4a pilus biogenesis protein PilO -
  CXB48_RS02955 (CXB48_02950) pilP 622738..623265 (+) 528 WP_095460897.1 type 4a pilus biogenesis lipoprotein PilP -
  CXB48_RS02960 (CXB48_02955) pilQ 623319..625418 (+) 2100 WP_041755270.1 type IV pilus secretin PilQ Machinery gene
  CXB48_RS02965 (CXB48_02960) aroK 625425..625970 (+) 546 WP_041755271.1 shikimate kinase AroK -
  CXB48_RS02970 (CXB48_02965) aroB 625994..627097 (+) 1104 WP_011911792.1 3-dehydroquinate synthase -
  CXB48_RS02975 (CXB48_02970) - 627108..628697 (+) 1590 WP_108241416.1 AAA family ATPase -

Sequence

Protein


Download         Length: 699 a.a.        Molecular weight: 75134.07 Da        Isoelectric Point: 4.7313

>NTDB_id=258666 CXB48_RS02960 WP_041755270.1 623319..625418(+) (pilQ) [Stutzerimonas stutzeri strain SGAir0442]
MNTTLSRLGVSLLAVFISPALLAANLNALDVAALPGDRVELKLAFDEPVASPRGYTLDQPARIALDLPGVSNNLGAKNRE
LGVGNARSVTVVEAQGRTRLIVNLTALVPYSTRVDGNNLFVVLGEGSGTTSPAAASAAVTAPIAKPAPVKPMAAGKAIDN
IDFRRGEDGAGNVVITLSDPSVGSTIEEQGGKIRVVFAKAELPEALRVRLDVQDFATPVKFVDSTAQAGGASIAIEPTGR
YDYLAYQTDNKLTISVKPLTEEEADKRKTDRFAYSGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVSGNITLRLQNV
PWDQALDLVLKTKGLDKRQVGNVLLVAPADEIAARERQELESQRQIAELAPLRREVVQVNYAKAADIARLFQSVTNNGGV
AEERGSIAVDDRTNNIIAYQTQERLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGNLGAGNKWSAWGKNG
NLDIEDGEDGGQQITWPMNTPFVDMGAVGSTSGIGLGFVTDNAILDLQLSAMEKTGNGEVVSQPKVVTADKETAKILKGS
EVPYQEASSSGATSTSFKEAALSLEVTPQITPDNRIIMEVKVTKDEPDFSQAATTGVPAIRKNEVNAKVLVTDGETIVIG
GVFSNTQSKSVDKVPFLGDLPYLGRLFRRDLVQDRKSELLVFITPRIMNNQSIQASRSY

Nucleotide


Download         Length: 2100 bp        

>NTDB_id=258666 CXB48_RS02960 WP_041755270.1 623319..625418(+) (pilQ) [Stutzerimonas stutzeri strain SGAir0442]
ATGAACACTACCCTTTCCCGTCTGGGCGTATCCCTGCTGGCGGTATTTATTTCGCCAGCACTGCTGGCGGCCAATCTCAA
CGCTTTGGATGTGGCTGCTTTGCCGGGCGATCGGGTTGAGTTGAAACTGGCATTTGACGAGCCTGTCGCGTCACCTCGTG
GCTACACGCTGGATCAGCCCGCGCGCATTGCCCTGGATCTTCCAGGTGTATCCAACAACCTGGGGGCTAAAAACCGAGAG
CTAGGCGTTGGTAATGCGCGCAGCGTAACGGTCGTCGAGGCTCAAGGGCGCACCCGGCTCATCGTCAATCTGACCGCGCT
TGTTCCGTATTCCACTCGAGTGGATGGGAACAATCTGTTTGTCGTGCTGGGAGAGGGCTCGGGGACGACCAGCCCTGCGG
CTGCATCCGCCGCGGTCACTGCTCCGATTGCCAAGCCTGCTCCGGTAAAGCCGATGGCGGCCGGCAAGGCGATCGACAAT
ATCGATTTTCGCCGCGGTGAAGATGGTGCCGGAAACGTGGTAATCACACTGTCCGATCCGTCGGTAGGTTCGACTATCGA
AGAGCAGGGAGGAAAGATCCGCGTTGTGTTCGCCAAGGCGGAGCTGCCTGAAGCGCTGCGTGTTCGGCTTGACGTCCAGG
ATTTCGCTACGCCGGTGAAGTTTGTCGACTCGACCGCTCAGGCAGGCGGTGCCAGTATCGCCATCGAGCCTACTGGGCGA
TACGATTATCTCGCCTACCAGACAGACAACAAGCTGACCATCAGCGTAAAGCCCCTTACAGAAGAAGAGGCCGATAAGCG
CAAGACAGACCGTTTTGCCTATTCCGGTGAAAAGCTGTCGCTGAACTTCCAAGATATTGATGTTCGTTCCGTACTCCAGT
TGATCGCTGATTTTACTGACCTGAACTTGGTTGCCAGCGATACCGTCAGTGGCAACATCACCCTTCGTTTGCAGAACGTG
CCTTGGGACCAAGCACTCGACCTCGTTTTGAAAACCAAAGGGCTGGATAAGCGGCAAGTCGGTAACGTACTGCTCGTTGC
GCCCGCTGATGAAATCGCGGCACGTGAACGCCAAGAGCTTGAGTCGCAGCGCCAAATTGCCGAGCTTGCACCGTTGCGTC
GCGAGGTTGTGCAGGTCAACTATGCCAAGGCTGCGGACATTGCCCGCCTGTTTCAGTCGGTGACGAACAATGGCGGCGTG
GCGGAAGAGCGTGGATCGATTGCGGTGGACGACCGTACTAACAACATCATCGCTTATCAAACGCAAGAGCGGCTAGATGA
GTTGCGTCGTATTGTTGCTCAGCTCGATATCCCGGTGCGGCAGGTCATGATCGAAGCGCGGATCGTCGAGGCGAACGTTG
ACTACGACAAGAGTCTCGGCGTTCGCTGGGGGGGAAATCTTGGAGCTGGCAACAAATGGAGCGCTTGGGGAAAGAACGGC
AATCTGGATATTGAGGATGGTGAAGACGGCGGCCAACAGATTACATGGCCTATGAATACGCCGTTTGTAGATATGGGGGC
TGTCGGTTCTACTTCTGGCATTGGACTCGGATTTGTAACGGATAACGCGATCCTTGACCTCCAGCTTTCGGCGATGGAGA
AGACGGGAAATGGCGAGGTGGTTTCTCAGCCTAAAGTGGTTACTGCCGATAAGGAAACTGCAAAAATTCTCAAAGGCTCG
GAAGTTCCCTATCAGGAAGCTAGTTCCAGTGGGGCAACTAGCACATCCTTTAAAGAGGCTGCGCTTTCGCTAGAGGTGAC
GCCGCAGATTACGCCGGATAACCGAATCATCATGGAAGTAAAGGTCACAAAGGATGAGCCGGATTTTTCACAGGCAGCGA
CAACCGGCGTGCCGGCTATCCGCAAGAATGAAGTGAACGCGAAAGTGCTTGTCACTGATGGCGAGACGATTGTTATCGGC
GGAGTCTTCTCTAATACGCAAAGCAAATCGGTCGATAAGGTGCCATTCCTTGGGGATTTGCCATATCTTGGACGCCTTTT
CCGTCGCGACCTAGTGCAAGATCGAAAATCTGAGCTGCTTGTGTTCATTACTCCTAGGATCATGAATAATCAGTCAATCC
AAGCTAGCCGCTCATATTGA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(38-123)

Secretin and TonB N terminus short domain (STN)

(302-348)

Bacterial type II/III secretion system short domain (Secretin_N)

(375-441)

Bacterial type II and III secretion system protein (Secretin)

(531-688)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

75.173

100

0.775
  pilQ Acinetobacter baumannii D1279779

39.892

100

0.423
  pilQ Acinetobacter baumannii strain A118

39.892

100

0.423
  comQ Acinetobacter baylyi ADP1

41.908

98.999

0.415
  pilQ Legionella pneumophila strain Lp02

37.518

96.853

0.363
  pilQ Legionella pneumophila strain ERS1305867

37.518

96.853

0.363

Multiple sequence alignment