Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   CWI64_RS05870 Genome accession   NZ_CP025076
Coordinates   1080315..1081331 (-) Length   338 a.a.
NCBI ID   WP_078132591.1    Uniprot ID   A0A0T8LTS4
Organism   Streptococcus pneumoniae strain BHN97x     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1075315..1086331
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CWI64_RS12295 - 1075385..1075516 (-) 132 WP_000768904.1 hypothetical protein -
  CWI64_RS05830 (CWI64_05830) - 1075517..1076707 (-) 1191 WP_000167766.1 acetate kinase -
  CWI64_RS05835 (CWI64_05835) comYH 1076758..1077711 (-) 954 WP_000345133.1 class I SAM-dependent methyltransferase Machinery gene
  CWI64_RS05840 (CWI64_05840) - 1077772..1078373 (-) 602 Protein_1078 methyltransferase domain-containing protein -
  CWI64_RS05845 (CWI64_05845) comGG/cglG 1078510..1078923 (-) 414 WP_000265622.1 competence type IV pilus minor pilin ComGG Machinery gene
  CWI64_RS05850 (CWI64_05850) comGF/cglF 1078901..1079362 (-) 462 WP_000250534.1 competence type IV pilus minor pilin ComGF Machinery gene
  CWI64_RS05855 (CWI64_05855) comGE/cglE 1079325..1079627 (-) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  CWI64_RS05860 (CWI64_05860) comGD/cglD 1079590..1079994 (-) 405 WP_000588024.1 competence type IV pilus minor pilin ComGD Machinery gene
  CWI64_RS05865 (CWI64_05865) comGC/cglC 1079987..1080313 (-) 327 WP_000738630.1 comG operon protein ComGC Machinery gene
  CWI64_RS05870 (CWI64_05870) comGB/cglB 1080315..1081331 (-) 1017 WP_078132591.1 competence type IV pilus assembly protein ComGB Machinery gene
  CWI64_RS05875 (CWI64_05875) comGA/cglA/cilD 1081279..1082220 (-) 942 WP_000249573.1 competence type IV pilus ATPase ComGA Machinery gene
  CWI64_RS05880 (CWI64_05880) - 1082296..1082661 (-) 366 WP_000286415.1 DUF1033 family protein -
  CWI64_RS05885 (CWI64_05885) - 1082812..1083870 (-) 1059 WP_000649468.1 zinc-dependent alcohol dehydrogenase family protein -
  CWI64_RS05890 (CWI64_05890) nagA 1084033..1085184 (-) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38518.56 Da        Isoelectric Point: 9.4803

>NTDB_id=258095 CWI64_RS05870 WP_078132591.1 1080315..1081331(-) (comGB/cglB) [Streptococcus pneumoniae strain BHN97x]
MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLDRSALLDKQCVTQMRVGLSQGKSFSEMMESLGCSSAI
VTQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATQIIGNLPQIF
LGMIVFVSVGVLLALTFYKRSSKMHVFSILARLPFIGIFVQTYLTAYYAREWGNMISQGMELTQIFQMMQEQGSQLFKEI
GQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=258095 CWI64_RS05870 WP_078132591.1 1080315..1081331(-) (comGB/cglB) [Streptococcus pneumoniae strain BHN97x]
ATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAAGCAAAAAAATATCATCACCCTATTTAA
CAATCTCTTTTCTAGCGGTTTTCATCTGGTGGAGACTATCTCCTTTTTAGATAGGAGTGCTTTGTTGGACAAGCAGTGTG
TGACCCAGATGCGTGTGGGCTTGTCTCAGGGGAAATCATTCTCAGAAATGATGGAAAGTTTGGGATGTTCAAGTGCTATT
GTCACTCAGTTATCCCTAGCTGAAGTTCATGGCAATCTCCACCTGAGTTTGGGAAAGATAGAAGAATATCTGGACAATCT
GGCCAAGGTCAAGAAAAAATTGATTGAAGTAGCGACCTATCCCTTGATTTTGCTGGGTTTTCTTCTCTTAATTATGCTGG
GGCTACGGAATTACCTGCTCCCACAACTGGATAGTAGCAATATTGCCACCCAAATTATCGGTAATCTGCCACAAATCTTT
CTAGGAATGATAGTTTTTGTTTCAGTAGGTGTCCTTTTAGCACTCACTTTTTATAAAAGAAGTTCTAAGATGCACGTCTT
TTCTATCTTAGCGCGCCTTCCTTTTATTGGAATCTTTGTGCAGACCTATTTGACAGCCTATTATGCACGTGAATGGGGGA
ATATGATTTCACAGGGAATGGAGTTGACGCAGATTTTTCAAATGATGCAGGAACAAGGTTCCCAGCTCTTTAAAGAAATC
GGTCAAGATCTGGCTCAAACCCTGAAAAATGGCCGTGAATTTTCTCAGACGATAGGAACCTATCCTTTCTTTAGGAAGGA
ATTGAGTCTCATCATAGAGTATGGGGAAGTTAAGTCCAAGCTGGGTAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG
AAGCCTTTTTTACCCGAGTCAACCGCACCATGAATTTGGTGCAGCCACTGGTTTTTATCTTTGTGGCACTGATTATCGTT
TTACTTTATGCGGCAATGCTCATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0T8LTS4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus pneumoniae Rx1

97.929

100

0.979
  comGB/cglB Streptococcus pneumoniae D39

97.929

100

0.979
  comGB/cglB Streptococcus pneumoniae R6

97.929

100

0.979
  comGB/cglB Streptococcus pneumoniae TIGR4

97.929

100

0.979
  comGB/cglB Streptococcus mitis NCTC 12261

95.266

100

0.953
  comGB/cglB Streptococcus mitis SK321

94.379

100

0.944
  comYB Streptococcus gordonii str. Challis substr. CH1

70.833

99.408

0.704
  comYB Streptococcus mutans UA140

58.621

94.379

0.553
  comYB Streptococcus mutans UA159

58.621

94.379

0.553
  comGB Lactococcus lactis subsp. cremoris KW2

50.299

98.817

0.497

Multiple sequence alignment