Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA/celA/cilE   Type   Machinery gene
Locus tag   CWI64_RS00400 Genome accession   NZ_CP025076
Coordinates   68252..68902 (+) Length   216 a.a.
NCBI ID   WP_000387344.1    Uniprot ID   A0A0T9GI09
Organism   Streptococcus pneumoniae strain BHN97x     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 63252..73902
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CWI64_RS00370 (CWI64_00370) - 64212..65026 (+) 815 Protein_70 IS5-like element IS1515 family transposase -
  CWI64_RS00380 (CWI64_00380) - 65209..65709 (+) 501 WP_000566988.1 GNAT family N-acetyltransferase -
  CWI64_RS00385 (CWI64_00385) - 65712..66038 (+) 327 Protein_72 TfoX/Sxy family protein -
  CWI64_RS00390 (CWI64_00390) ald 66339..67438 (-) 1100 Protein_73 alanine dehydrogenase -
  CWI64_RS00395 (CWI64_00395) - 67615..68184 (+) 570 WP_000443750.1 GNAT family N-acetyltransferase -
  CWI64_RS00400 (CWI64_00400) comEA/celA/cilE 68252..68902 (+) 651 WP_000387344.1 helix-hairpin-helix domain-containing protein Machinery gene
  CWI64_RS00405 (CWI64_00405) comEC/celB 68886..71126 (+) 2241 WP_000942394.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  CWI64_RS00410 (CWI64_00410) - 71305..71493 (+) 189 WP_001808514.1 hypothetical protein -
  CWI64_RS00415 (CWI64_00415) - 71526..72113 (+) 588 WP_000939895.1 ABC transporter ATP-binding protein -
  CWI64_RS00420 (CWI64_00420) - 72117..73301 (+) 1185 WP_000655960.1 ABC transporter permease -

Sequence

Protein


Download         Length: 216 a.a.        Molecular weight: 23181.48 Da        Isoelectric Point: 6.0746

>NTDB_id=258055 CWI64_RS00400 WP_000387344.1 68252..68902(+) (comEA/celA/cilE) [Streptococcus pneumoniae strain BHN97x]
MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTPVKETNLQAEVAAVSKDSSTEKEVKKEEKEEPLEQDLITVDVKG
AVKSPGIYDLPVGSRVNDAVQKAGGLTEQADSKSLNLAQKVSDEALVYVPTKGEEAVSQQTGSGTASSTSKEKKVNLNKA
SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGGKTIEKLKDYVTVD

Nucleotide


Download         Length: 651 bp        

>NTDB_id=258055 CWI64_RS00400 WP_000387344.1 68252..68902(+) (comEA/celA/cilE) [Streptococcus pneumoniae strain BHN97x]
ATGGAAGCAATTATCGAGAAAATCAAAGAGTATAAAATCATCGTCATCTGTACTGGTCTGGGCTTGCTTGTAGGAGGATT
TTTCCTGCTAAAACCAGCTCCACAAACACCTGTCAAAGAGACGAATTTGCAGGCTGAAGTTGCAGCTGTTTCCAAGGACT
CATCGACCGAAAAGGAAGTGAAGAAGGAAGAAAAGGAAGAACCCCTTGAACAAGATCTAATCACAGTAGATGTCAAAGGT
GCTGTCAAATCGCCAGGGATTTATGACTTGCCTGTAGGTAGTCGAGTCAATGATGCTGTTCAGAAGGCTGGTGGCTTGAC
AGAGCAAGCAGACAGCAAGTCGCTCAATCTAGCTCAGAAAGTTAGTGATGAGGCTCTGGTTTACGTTCCTACTAAGGGAG
AAGAAGCAGTTAGTCAACAGACTGGTTCGGGGACAGCTTCTTCAACAAGCAAGGAAAAGAAGGTCAATCTCAACAAGGCC
AGTCTGGAAGAACTCAAGCAGGTCAAGGGACTGGGAGGAAAACGAGCTCAGGACATTATTGACCATCGTGAGGCAAATGG
CAAGTTCAAGTCAGTAGACGAGCTCAAGAAGGTCTCTGGCATTGGTGGCAAAACAATAGAAAAGCTTAAAGACTATGTTA
CAGTGGATTAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(151-214)

SLBB domain (SLBB)

(76-126)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0T9GI09

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

100

100

1
  comEA/celA/cilE Streptococcus pneumoniae Rx1

97.222

100

0.972
  comEA/celA/cilE Streptococcus pneumoniae D39

97.222

100

0.972
  comEA/celA/cilE Streptococcus pneumoniae R6

97.222

100

0.972
  comEA/celA/cilE Streptococcus mitis NCTC 12261

96.759

100

0.968
  comEA/celA/cilE Streptococcus mitis SK321

93.056

100

0.931
  comEA Lactococcus lactis subsp. cremoris KW2

41.778

100

0.435
  comEA Latilactobacillus sakei subsp. sakei 23K

34.335

100

0.37

Multiple sequence alignment