Detailed information NTG0258056

Bioinformatically predicted

Overview

• Name: comEC/celB
• Type: Machinery gene
• Locus tag: CWI64_RS00405
• Genome accession: NZ_CP025076
• Coordinates: 68886..71126 (+)
• Length: 746 a.a.
• NCBI ID: WP_000942394.1
• Uniprot ID: A0A166WJ67
• Organism: Streptococcus pneumoniae strain BHN97x
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 63886..76126

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CWI64_RS00370 (CWI64_00370)	-	64212..65026 (+)	815	Protein_70	IS5-like element IS1515 family transposase	-
CWI64_RS00380 (CWI64_00380)	-	65209..65709 (+)	501	WP_000566988.1	GNAT family N-acetyltransferase	-
CWI64_RS00385 (CWI64_00385)	-	65712..66038 (+)	327	Protein_72	TfoX/Sxy family protein	-
CWI64_RS00390 (CWI64_00390)	ald	66339..67438 (-)	1100	Protein_73	alanine dehydrogenase	-
CWI64_RS00395 (CWI64_00395)	-	67615..68184 (+)	570	WP_000443750.1	GNAT family N-acetyltransferase	-
CWI64_RS00400 (CWI64_00400)	comEA/celA/cilE	68252..68902 (+)	651	WP_000387344.1	helix-hairpin-helix domain-containing protein	Machinery gene
CWI64_RS00405 (CWI64_00405)	comEC/celB	68886..71126 (+)	2241	WP_000942394.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
CWI64_RS00410 (CWI64_00410)	-	71305..71493 (+)	189	WP_001808514.1	hypothetical protein	-
CWI64_RS00415 (CWI64_00415)	-	71526..72113 (+)	588	WP_000939895.1	ABC transporter ATP-binding protein	-
CWI64_RS00420 (CWI64_00420)	-	72117..73301 (+)	1185	WP_000655960.1	ABC transporter permease	-
CWI64_RS00425 (CWI64_00425)	infC	73612..74142 (+)	531	WP_000848180.1	translation initiation factor IF-3	-
CWI64_RS00430 (CWI64_00430)	rpmI	74175..74375 (+)	201	WP_001125943.1	50S ribosomal protein L35	-
CWI64_RS00435 (CWI64_00435)	rplT	74427..74786 (+)	360	WP_000124836.1	50S ribosomal protein L20	-
CWI64_RS00440 (CWI64_00440)	-	74844..75224 (+)	381	WP_000157154.1	VOC family protein	-

Sequence

Protein

• Length: 746 a.a.
• Molecular weight: 84444.00 Da
• Isoelectric Point: 9.6202

>NTDB_id=258056 CWI64_RS00405 WP_000942394.1 68886..71126(+) (comEC/celB) [Streptococcus pneumoniae strain BHN97x]
MLQWIKNFSIPLIYLSFLLLWLYYAIFSASYLALLGFVFLLVCLFIQFPWKSAGKVLIICGIFGFWFVFQNWQQSQASQN
LADSVERVRILPDTIKVNGDSLSFRGKSNGRAFQVYYKLQSEEEKEAFQALTDLHEIGLEGKLSEPEGQRNFGGFNYQAY
LKTQGIYQTLNIKTIQSLQKIGSWDIGENLSSLRRKAVVWIKTHFPDPMGNYMTGLLLGHLDTDFEEMNELYSSLGIIHL
FALSGMQVGFFMNGFKKLLLRLGLTQEKLKWLTYPFSLIYAGLTGFSASVIRSLLQKLLAQHGVKGLDNFALTVLVLFIV
MPNFFLTAGGVLSCAYAFILTMTSKEGEGLKAVTSESLVISLGILPILSFYFAEFQPWSILLTFVFSFLFDLVFLPLLSI
LFVLSFLYPVIQLNFIFEWLEGIIRLVSQVARRPLVFGQPNAWLLILLLISLALVYDLRKNIKGLTVLSLLITGLFFLTK
YPLENEITMLDVGQGESIFLRDVTGKTILIDVGGKAESYKKIKKWQEKMTTSNAQRTLIPYLKSRGVAKIDQLILTNTDK
EHVGDLSEMTKAFHVGEILVSKDSLKQKEFVAELQATQTKVRSMIVGENLPIFGSQLEVLSPRKMGDGGHDDTLVLYGKF
LDKQFLFTGNLEEKGEKDLLKHYPDLKVNVLKASQHGNKKSSSPAFLEKLKPELTLISVGKSNRMKLPHQETLTRLEGIN
SKVYRTDQQGAIRFKGLDSWKIESVR

Nucleotide

• Length: 2241 bp

>NTDB_id=258056 CWI64_RS00405 WP_000942394.1 68886..71126(+) (comEC/celB) [Streptococcus pneumoniae strain BHN97x]
ATGTTACAGTGGATTAAGAATTTCTCTATTCCCCTAATTTACCTGAGTTTTCTATTACTTTGGCTTTATTACGCTATTTT
CTCAGCATCTTATCTTGCTTTGTTGGGCTTTGTTTTTCTGCTAGTCTGTCTCTTTATCCAATTTCCGTGGAAATCTGCTG
GTAAAGTTCTAATAATTTGCGGAATCTTTGGATTTTGGTTTGTTTTTCAAAATTGGCAACAGAGTCAAGCGAGTCAAAAT
CTGGCGGATTCTGTTGAAAGGGTACGGATTTTGCCTGATACTATTAAGGTTAATGGTGATAGTCTATCCTTTCGTGGCAA
GTCTAACGGTCGTGCTTTCCAAGTCTATTATAAACTCCAGTCCGAGGAGGAGAAAGAAGCCTTTCAAGCTTTAACTGACC
TGCATGAGATAGGACTAGAAGGGAAGCTTTCGGAGCCAGAAGGGCAGAGAAATTTTGGTGGCTTTAATTACCAAGCCTAT
CTGAAGACTCAGGGAATTTACCAGACTCTCAATATCAAAACAATCCAGTCACTTCAAAAGATTGGCAGTTGGGATATAGG
AGAAAACTTGTCCAGTTTACGTCGAAAGGCTGTGGTTTGGATTAAGACGCACTTTCCAGACCCTATGGGCAATTACATGA
CAGGACTCTTGCTGGGACATCTGGACACCGACTTTGAGGAGATGAATGAGCTTTATTCCAGTCTAGGAATTATCCACCTC
TTTGCCCTATCTGGCATGCAGGTAGGTTTTTTCATGAATGGATTTAAGAAACTTCTCTTGCGATTGGGCTTGACCCAAGA
AAAGTTGAAATGGCTGACTTATCCCTTTTCCCTTATCTATGCGGGACTAACTGGATTTTCAGCATCGGTTATTCGCAGTC
TCTTGCAAAAGCTACTGGCTCAACATGGGGTTAAGGGCTTGGATAATTTTGCCTTGACGGTGCTTGTCCTCTTTATTGTC
ATGCCAAACTTTTTCTTGACAGCAGGAGGAGTCTTGTCCTGCGCTTATGCTTTTATCCTGACCATGACCAGCAAAGAAGG
GGAGGGGCTCAAGGCTGTTACTAGTGAAAGTCTAGTCATCTCCTTGGGCATATTGCCCATTCTATCCTTCTATTTTGCGG
AATTTCAACCTTGGTCTATCCTTTTGACCTTTGTCTTTTCCTTTCTTTTTGACTTGGTCTTCTTACCGCTCTTGTCTATC
TTATTTGTCCTTTCCTTTCTCTATCCAGTCATTCAGCTGAACTTTATCTTTGAATGGTTAGAGGGCATTATTCGCTTGGT
CTCGCAGGTGGCAAGGAGACCACTTGTCTTTGGTCAACCCAACGCATGGCTTTTAATCTTATTGTTAATTTCCTTGGCTT
TGGTCTATGATTTGAGGAAAAACATTAAAGGATTAACAGTATTGAGTTTATTGATTACAGGTCTCTTTTTCCTTACCAAG
TATCCACTGGAAAATGAAATCACCATGCTGGATGTGGGGCAAGGAGAAAGTATTTTCCTACGGGATGTAACTGGGAAAAC
CATTCTCATAGATGTAGGTGGTAAGGCAGAATCTTATAAGAAAATCAAAAAATGGCAAGAAAAGATGACGACCAGCAATG
CCCAGCGAACCTTGATTCCCTATCTCAAAAGTCGAGGAGTAGCTAAGATTGACCAGCTAATTTTGACTAACACGGACAAG
GAGCATGTTGGAGATTTGTCAGAGATGACCAAGGCTTTCCATGTAGGGGAGATTCTAGTATCAAAAGACAGTCTGAAACA
GAAGGAATTTGTGGCAGAACTACAGGCGACTCAAACAAAGGTGCGTAGTATGATAGTAGGGGAGAACTTGCCCATTTTTG
GAAGTCAGTTAGAAGTTCTATCTCCAAGGAAAATGGGAGATGGAGGACACGATGATACCCTAGTTCTGTATGGGAAATTC
TTGGATAAGCAATTTCTCTTCACGGGAAATTTGGAGGAGAAAGGAGAGAAGGACTTGCTGAAGCACTATCCAGACTTGAA
AGTAAATGTTTTGAAAGCTAGCCAACATGGCAATAAAAAATCATCAAGTCCAGCCTTTCTAGAAAAACTCAAACCAGAGC
TTACTCTTATCTCAGTTGGAAAGAGCAATCGAATGAAACTCCCCCATCAGGAAACATTGACACGACTGGAAGGTATCAAT
AGCAAAGTTTATCGAACTGACCAGCAAGGAGCTATACGTTTTAAGGGGTTGGATAGTTGGAAAATCGAAAGTGTTCGATA
G

Domains

Predicted by InterproScan.

• Competence protein (Competence) (216-458)

• Domain of unknown function (DUF4131) (DUF4131) (30-176)

• Metallo-beta-lactamase superfamily (Lactamase_B) (492-693)

3D structure

• AlphaFold DB: A0A166WJ67

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEC/celB	Streptococcus pneumoniae TIGR4	100	100	1
comEC/celB	Streptococcus pneumoniae Rx1	97.587	100	0.976
comEC/celB	Streptococcus pneumoniae D39	97.587	100	0.976
comEC/celB	Streptococcus pneumoniae R6	97.587	100	0.976
comEC/celB	Streptococcus mitis SK321	92.225	100	0.922
comEC/celB	Streptococcus mitis NCTC 12261	91.812	99.866	0.917
comEC	Lactococcus lactis subsp. cremoris KW2	44.399	99.33	0.441