Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CS557_RS13825 Genome accession   NZ_CP024632
Coordinates   2939773..2941497 (+) Length   574 a.a.
NCBI ID   WP_099949291.1    Uniprot ID   -
Organism   Acinetobacter junii strain lzh-X15     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2934773..2946497
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CS557_RS13790 (CS557_13795) nusA 2935625..2937109 (-) 1485 WP_004916840.1 transcription termination factor NusA -
  CS557_RS13795 (CS557_13800) rimP 2937147..2937671 (-) 525 WP_004916844.1 ribosome maturation factor RimP -
  CS557_RS13815 (CS557_13820) secG 2938294..2938623 (-) 330 WP_004916847.1 preprotein translocase subunit SecG -
  CS557_RS13820 (CS557_13825) tpiA 2938636..2939427 (-) 792 WP_099949290.1 triose-phosphate isomerase -
  CS557_RS13825 (CS557_13830) pilB 2939773..2941497 (+) 1725 WP_099949291.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CS557_RS13830 (CS557_13835) pilC 2941522..2942751 (+) 1230 WP_099949292.1 type II secretion system F family protein Machinery gene
  CS557_RS13835 (CS557_13840) pilD 2942764..2943624 (+) 861 WP_099949293.1 prepilin peptidase Machinery gene
  CS557_RS13840 (CS557_13845) coaE 2943626..2944225 (+) 600 WP_004957489.1 dephospho-CoA kinase -
  CS557_RS13845 (CS557_13850) - 2944222..2945133 (-) 912 WP_099949294.1 DMT family transporter -
  CS557_RS13850 (CS557_13855) rlmB 2945164..2945913 (-) 750 WP_099949295.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  CS557_RS13855 (CS557_13860) - 2946042..2946368 (-) 327 WP_004958605.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 63650.78 Da        Isoelectric Point: 5.0662

>NTDB_id=254599 CS557_RS13825 WP_099949291.1 2939773..2941497(+) (pilB) [Acinetobacter junii strain lzh-X15]
MSGIHTSPKFSGFIRRIVDDGHIAASNMQSAIDAAKKAQQDIVAHLIEHHRLSPLTIAESISLEFGEPLFDLAVFDTALL
PREITDSKLIQKHRVVPLIQRGQILYVATSNPTNIDALDAIRFNTKLNIEPVIVEHNKLEKLIDQQFGDSGSFDFGENDD
FDLDNVDVASTDTNNTTEDEDTNNQEESPIVKYINKLLIDAIRMGASDLHFEPYEKMYRVRYRVDGVLRQIANPPLQLAT
RLASRLKVMSQMDISEKRMPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIEALGYEDEQKELFMSA
LDKPQGMLLITGPTGSGKTVSLYTGLNILNTEDTNISTAEDPVEINLEGINQVNVNPKTGLTFSAALKAFLRQDPDVIMV
GEIRDLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSECKQPIEIPKQSLL
EMGFTEQDLANPDFQIFQPVGCDACREGYKGRVGIYEVMKVTPEISKIIMEDGNALQIAAASEKAGFNNLRRSGLKKVMQ
GITSLQEVNRVTSE

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=254599 CS557_RS13825 WP_099949291.1 2939773..2941497(+) (pilB) [Acinetobacter junii strain lzh-X15]
ATGTCAGGAATACATACGTCTCCAAAATTTTCGGGGTTTATTCGACGCATAGTCGATGATGGTCACATTGCTGCATCAAA
TATGCAGAGTGCGATTGATGCTGCAAAAAAAGCACAACAAGATATTGTTGCTCACTTAATTGAACATCATCGTCTATCAC
CTCTGACAATTGCGGAAAGCATATCTTTAGAGTTTGGTGAGCCACTCTTTGATCTTGCTGTATTTGATACAGCTCTATTA
CCTCGTGAAATAACTGACAGCAAACTGATTCAAAAACATCGTGTTGTTCCACTCATTCAACGTGGTCAGATTTTGTATGT
TGCGACCAGCAACCCGACGAATATTGATGCATTAGACGCAATCAGGTTCAATACCAAATTGAATATCGAACCTGTGATTG
TTGAGCACAATAAACTCGAAAAACTGATTGATCAGCAATTTGGAGATAGCGGCAGTTTCGATTTTGGGGAAAATGACGAC
TTTGACTTAGATAATGTTGATGTTGCATCGACTGATACAAACAATACGACTGAGGATGAGGATACAAATAATCAAGAAGA
ATCCCCTATCGTCAAATATATCAACAAGCTATTGATTGATGCGATTCGCATGGGAGCCTCTGACTTACATTTTGAGCCTT
ATGAAAAAATGTATCGCGTGCGTTATCGTGTGGATGGTGTACTCAGACAAATTGCTAACCCACCATTACAACTCGCAACT
CGTTTAGCCTCTCGCTTAAAAGTCATGTCTCAAATGGACATTTCTGAAAAGCGTATGCCTCAAGATGGTCGTATCAAACT
AAAACTCTCTAAAACTAAAGCGATTGATTTTCGTGTTAACTCACTTCCAACTTTATTTGGAGAGAAACTGGTTCTTCGTA
TTTTAGATCCTTCAAGCGCAATGTTAGGTATTGAAGCCTTAGGCTATGAGGATGAACAAAAAGAACTATTTATGAGTGCA
TTAGACAAGCCTCAGGGTATGCTTCTGATTACAGGTCCGACAGGTTCTGGTAAAACCGTATCGTTATATACAGGTCTAAA
TATCTTAAACACAGAAGATACCAATATTTCGACCGCAGAAGATCCCGTCGAAATTAACTTAGAAGGTATTAACCAAGTTA
ACGTCAACCCTAAAACAGGTTTAACTTTTTCAGCAGCATTAAAAGCTTTTTTACGTCAAGATCCTGATGTCATCATGGTC
GGTGAGATTCGTGATTTGGAAACAGCTGAAATTGCAATTAAAGCAGCTCAAACAGGTCACATGGTGATGTCGACCCTACA
TACCAACAGCGCTCCTGAAACACTCACACGATTACGCAATATGGGGGTTCCATCTTTCAATATTGCAACTTCTGTTAATC
TAGTGATTGCTCAACGTTTAGCTCGTCGTCTATGCTCGGAATGCAAACAACCTATTGAGATTCCCAAGCAAAGCCTTTTA
GAAATGGGCTTCACTGAACAAGATTTAGCCAACCCTGACTTTCAAATTTTCCAACCTGTCGGCTGCGATGCTTGTCGTGA
AGGATATAAAGGGCGTGTCGGTATTTATGAGGTCATGAAAGTGACACCCGAGATTTCCAAGATTATTATGGAAGATGGAA
ATGCATTACAAATTGCAGCAGCATCTGAAAAGGCTGGTTTTAATAATCTACGCCGTTCTGGTTTAAAAAAGGTCATGCAA
GGGATCACCTCTTTGCAAGAAGTTAACCGAGTAACAAGTGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-149)

Type II/IV secretion system protein (T2SSE)

(196-463)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

81.391

100

0.815
  pilB Acinetobacter baylyi ADP1

76.696

100

0.768
  pilB Legionella pneumophila strain ERS1305867

52.974

93.728

0.497
  pilB Vibrio parahaemolyticus RIMD 2210633

50

97.213

0.486
  pilF Neisseria gonorrhoeae MS11

50.457

95.296

0.481
  pilB Vibrio cholerae strain A1552

49.097

96.516

0.474
  pilB Vibrio campbellii strain DS40M4

51.823

90.767

0.47
  pilB/pilB1 Synechocystis sp. PCC 6803

34.903

100

0.375

Multiple sequence alignment