Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA/celA/cilE   Type   Machinery gene
Locus tag   DKY66_RS04350 Genome accession   NZ_CP029561
Coordinates   777442..778095 (+) Length   217 a.a.
NCBI ID   WP_000461741.1    Uniprot ID   -
Organism   Streptococcus agalactiae strain 32790-3A     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 772442..783095
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DKY66_RS04325 (DKY66_04320) - 772610..773440 (+) 831 WP_000735510.1 MetQ/NlpA family ABC transporter substrate-binding protein -
  DKY66_RS04330 (DKY66_04325) - 773675..775261 (+) 1587 WP_000673086.1 DEAD/DEAH box helicase -
  DKY66_RS04335 (DKY66_04330) - 775444..775710 (-) 267 WP_000598735.1 GIY-YIG nuclease family protein -
  DKY66_RS04340 (DKY66_04335) - 775703..776467 (-) 765 WP_000567428.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  DKY66_RS04345 (DKY66_04340) - 776602..777342 (+) 741 WP_000500222.1 1-acyl-sn-glycerol-3-phosphate acyltransferase -
  DKY66_RS04350 (DKY66_04345) comEA/celA/cilE 777442..778095 (+) 654 WP_000461741.1 helix-hairpin-helix domain-containing protein Machinery gene
  DKY66_RS04355 (DKY66_04350) comEC/celB 778079..780316 (+) 2238 WP_000902956.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  DKY66_RS04360 (DKY66_04355) - 780442..781251 (+) 810 WP_000153219.1 Cof-type HAD-IIB family hydrolase -
  DKY66_RS04365 (DKY66_04360) - 781262..782206 (+) 945 WP_000200834.1 LacI family DNA-binding transcriptional regulator -

Sequence

Protein


Download         Length: 217 a.a.        Molecular weight: 23714.05 Da        Isoelectric Point: 9.2366

>NTDB_id=254295 DKY66_RS04350 WP_000461741.1 777442..778095(+) (comEA/celA/cilE) [Streptococcus agalactiae strain 32790-3A]
MFEIVLEKIKSHKWETTGIIVGLLLFGILGLNHFGTHHKEDDLNINLEKKVSTITEKKVPMISHVKDKVSNQVTVDVKGA
VNHPGVYSLPSQSRVTDAIKRAGGLSNLADSKSVNLAQKLQDETVIYVAQKGEKITVVEEEKANNIATQGNSKGKINLNK
ADLSSLQTISGVGAKRAQDILDYRDSQGGFKTIDDLKNVSGIGEKTLEKLRQDVTLD

Nucleotide


Download         Length: 654 bp        

>NTDB_id=254295 DKY66_RS04350 WP_000461741.1 777442..778095(+) (comEA/celA/cilE) [Streptococcus agalactiae strain 32790-3A]
ATGTTTGAAATAGTATTGGAAAAAATTAAATCGCATAAATGGGAGACAACAGGAATTATTGTAGGACTCTTATTGTTTGG
AATTTTGGGTTTAAATCATTTTGGCACTCATCATAAAGAAGACGATTTAAATATCAATTTAGAGAAAAAAGTATCAACTA
TAACAGAAAAAAAGGTACCAATGATTAGTCACGTCAAAGATAAAGTATCTAATCAAGTAACGGTTGACGTAAAAGGGGCA
GTCAATCATCCAGGTGTTTATAGTTTGCCTTCTCAAAGTCGTGTAACAGATGCGATTAAGCGTGCAGGTGGACTAAGCAA
TCTCGCTGATAGTAAATCCGTTAATCTAGCACAGAAATTACAAGATGAGACGGTTATCTATGTTGCTCAAAAAGGTGAAA
AAATAACTGTTGTGGAAGAGGAGAAAGCAAATAATATTGCTACTCAGGGAAATAGTAAGGGGAAAATTAATCTTAATAAA
GCAGATTTATCAAGTCTTCAGACCATATCAGGCGTTGGAGCTAAAAGAGCTCAAGATATTTTAGATTATCGTGATAGTCA
AGGTGGTTTTAAAACTATTGATGACCTCAAAAATGTATCAGGTATTGGTGAGAAAACACTAGAAAAATTACGTCAAGATG
TTACACTTGACTAA

Domains

Predicted by InterProScan.

Helix-hairpin-helix motif (HHH_3)

(153-215)

SLBB domain (SLBB)

(75-128)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

44.595

100

0.456
  comEA/celA/cilE Streptococcus mitis SK321

44.144

100

0.452
  comEA/celA/cilE Streptococcus pneumoniae Rx1

43.694

100

0.447
  comEA/celA/cilE Streptococcus pneumoniae D39

43.694

100

0.447
  comEA/celA/cilE Streptococcus pneumoniae R6

43.694

100

0.447
  comEA/celA/cilE Streptococcus mitis NCTC 12261

43.243

100

0.442
  comEA Lactococcus lactis subsp. cremoris KW2

38.565

100

0.396
  comEA Streptococcus thermophilus LMD-9

56

69.124

0.387