Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CS058_RS24970 Genome accession   NZ_CP024477
Coordinates   5239313..5241013 (+) Length   566 a.a.
NCBI ID   WP_099887683.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain 12939     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5234313..5246013
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CS058_RS24950 nadC 5236118..5236966 (+) 849 WP_023098057.1 carboxylating nicotinate-nucleotide diphosphorylase -
  CS058_RS24960 - 5237147..5238460 (-) 1314 WP_237754601.1 O-antigen ligase family protein -
  CS058_RS24965 - 5238618..5239082 (-) 465 WP_023129290.1 pilin -
  CS058_RS24970 pilB 5239313..5241013 (+) 1701 WP_099887683.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CS058_RS24975 pilC 5241017..5242234 (+) 1218 WP_003161763.1 type II secretion system F family protein Machinery gene
  CS058_RS24980 pilD 5242235..5243107 (+) 873 WP_023084740.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  CS058_RS24985 coaE 5243104..5243715 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  CS058_RS24990 yacG 5243712..5243912 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  CS058_RS24995 - 5243949..5244158 (-) 210 WP_003094660.1 hypothetical protein -
  CS058_RS25000 - 5244264..5244953 (-) 690 WP_003103868.1 energy-coupling factor ABC transporter permease -
  CS058_RS25005 - 5244950..5245420 (-) 471 WP_003094664.1 hypothetical protein -
  CS058_RS25010 - 5245417..5245842 (-) 426 WP_003103865.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62306.21 Da        Isoelectric Point: 5.5785

>NTDB_id=253672 CS058_RS24970 WP_099887683.1 5239313..5241013(+) (pilB) [Pseudomonas aeruginosa strain 12939]
MNDSTPLSGLAKQLVLNQLLDEKTARQAQVQAARNKQSLVSHLVQAKLVQSRALVEVAAEQFGIAYCDLNSLDRESIPKD
VISERLVRQHRVLPLWKRGNKLFIGLSDPTNHQAVTDIQFSTGLTTEALLVEDDKLGVAIEKLYEGAMDALGDLGDIDLD
GVDVGKAESASQEDSGDDAEDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKTYRVRFRTDGMLHEVAKPPIQLASRISARL
KVMAGLDISERRKPQDGRIKMRVSKNKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHDVPKETLLHEGFPE
ELIGTFKLYSPVGCENCKGGNKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEGFNDLRTSGLLKAMQGITSLEEV
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=253672 CS058_RS24970 WP_099887683.1 5239313..5241013(+) (pilB) [Pseudomonas aeruginosa strain 12939]
ATGAACGATTCCACGCCTCTCAGCGGCCTTGCCAAGCAACTGGTCCTGAACCAACTCCTCGACGAAAAGACCGCCCGCCA
GGCCCAGGTGCAGGCCGCGCGCAACAAGCAGTCGCTGGTCAGCCACCTGGTCCAGGCCAAGCTGGTGCAGAGCCGGGCGT
TGGTCGAGGTGGCCGCCGAGCAGTTCGGCATCGCCTATTGCGACCTGAACAGCCTGGATCGGGAAAGCATTCCGAAGGAT
GTCATCAGCGAGCGCCTGGTCCGCCAGCACCGCGTGTTGCCGCTGTGGAAACGCGGCAACAAACTGTTCATCGGTCTCTC
CGACCCGACCAACCACCAGGCGGTCACCGATATCCAGTTCAGCACCGGTCTCACCACCGAAGCCCTGCTGGTGGAGGACG
ACAAGCTCGGCGTAGCCATCGAGAAGCTCTACGAGGGCGCTATGGACGCTCTCGGCGACCTCGGTGACATCGATCTCGAC
GGGGTCGATGTCGGCAAGGCGGAAAGCGCCAGCCAGGAAGATTCCGGCGACGACGCCGAAGATGCCCCGGTGGTGCGCTT
CGTCAACAAGATGCTGCTCGACGCCATCCGCGGCGGCTCTTCCGACCTGCACTTCGAACCCTACGAAAAGACCTACCGGG
TGCGCTTCCGTACCGACGGCATGCTCCACGAGGTAGCCAAGCCGCCGATCCAGCTGGCCAGCCGCATTTCCGCGCGGCTC
AAGGTGATGGCCGGCCTGGATATCTCCGAACGACGCAAGCCGCAGGACGGGCGGATCAAGATGCGCGTGTCGAAGAACAA
GTCCATCGACTTCCGCGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGTATCCTCGATTCCTCCAGCG
CGCAGATGGGCATCGACGCCCTCGGCTACGAGGAAGACCAGAAGGAACTCTACCTGGCTGCGCTCAAGCAGCCGCAGGGC
ATGATCCTGGTCACCGGGCCCACCGGCTCGGGCAAGACGGTCTCCCTGTACACCGGCCTGAACATCCTCAACACCACCGA
CATCAACATCTCCACTGCCGAAGACCCGGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAATCCGCGCCAGG
GCATGGACTTCTCCCAGGCGCTACGCGCCTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGACCTG
GAGACTGCCGAGATCGCCATCAAGGCGGCGCAGACCGGGCATATGGTGATGTCCACCCTGCACACCAACAGCGCCGCCGA
GACCCTGACCCGCCTGCTGAACATGGGCGTGCCGGCGTTCAACCTGGCGACCTCGGTGAACCTGATCATCGCCCAGCGCC
TTGCGCGAAAACTCTGTTCGCACTGCAAGAAAGAACACGACGTGCCGAAGGAAACCCTGCTTCACGAAGGTTTCCCCGAA
GAGCTGATCGGCACCTTCAAGCTGTATTCGCCTGTAGGCTGCGAAAACTGCAAAGGCGGTAACAAAGGTCGCGTCGGTAT
TTATGAAGTGGTTAAAAACACCCCCGCCCTGCAGCGGATTATCATGGAAGAAGGCAACTCCATCGAGATCGCCGAGCAAG
CCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCAGGCCTGCTGAAAGCCATGCAGGGAATCACCAGCCTGGAGGAAGTC
AACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.544

100

0.58
  pilB Acinetobacter baylyi ADP1

56.437

100

0.565
  pilB Legionella pneumophila strain ERS1305867

54.466

100

0.549
  pilB Vibrio cholerae strain A1552

51.521

98.763

0.509
  pilF Neisseria gonorrhoeae MS11

50.355

99.647

0.502
  pilB Vibrio parahaemolyticus RIMD 2210633

52.158

94.17

0.491
  pilB Vibrio campbellii strain DS40M4

51.22

94.17

0.482
  pilB/pilB1 Synechocystis sp. PCC 6803

37.601

100

0.41
  pilF Thermus thermophilus HB27

38.489

100

0.387
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.727

97.173

0.376

Multiple sequence alignment