Detailed information NTG0253674

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: CS058_RS24980
• Genome accession: NZ_CP024477
• Coordinates: 5242235..5243107 (+)
• Length: 290 a.a.
• NCBI ID: WP_023084740.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa strain 12939
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5237235..5248107

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CS058_RS24965	-	5238618..5239082 (-)	465	WP_023129290.1	pilin	-
CS058_RS24970	pilB	5239313..5241013 (+)	1701	WP_099887683.1	type IV-A pilus assembly ATPase PilB	Machinery gene
CS058_RS24975	pilC	5241017..5242234 (+)	1218	WP_003161763.1	type II secretion system F family protein	Machinery gene
CS058_RS24980	pilD	5242235..5243107 (+)	873	WP_023084740.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
CS058_RS24985	coaE	5243104..5243715 (+)	612	WP_003094654.1	dephospho-CoA kinase	-
CS058_RS24990	yacG	5243712..5243912 (+)	201	WP_003094656.1	DNA gyrase inhibitor YacG	-
CS058_RS24995	-	5243949..5244158 (-)	210	WP_003094660.1	hypothetical protein	-
CS058_RS25000	-	5244264..5244953 (-)	690	WP_003103868.1	energy-coupling factor ABC transporter permease	-
CS058_RS25005	-	5244950..5245420 (-)	471	WP_003094664.1	hypothetical protein	-
CS058_RS25010	-	5245417..5245842 (-)	426	WP_003103865.1	GNAT family N-acetyltransferase	-
CS058_RS25015	-	5245975..5246604 (+)	630	WP_003094668.1	DUF1780 domain-containing protein	-
CS058_RS25020	-	5246601..5247050 (+)	450	WP_003094670.1	MOSC domain-containing protein	-
CS058_RS25025	-	5247076..5247246 (+)	171	WP_003094672.1	DUF3094 family protein	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 31843.88 Da
• Isoelectric Point: 7.7068

>NTDB_id=253674 CS058_RS24980 WP_023084740.1 5242235..5243107(+) (pilD) [Pseudomonas aeruginosa strain 12939]
MTLLDYLASHPLAFVLCAILLGLLVGSFLNVVVHRLPKMMERNWKAEAREALGLEPEPKQATYNLVLPNSACPRCGHEIR
PWENIPLVSYLALGGKCSSCKAAIGKRYPLVELATALLSGYVAWHFGFTWQAGAMLLLTWGLLAMSLIDADHQLLPDVLV
LPLLWLGLIANHFGLFASLDDALFGAVFGYLSLWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AILGVIMLRLRNAESGTPIPFGPYLAIAGWIALLWGDQITRTYLQFAGFK

Nucleotide

• Length: 873 bp

>NTDB_id=253674 CS058_RS24980 WP_023084740.1 5242235..5243107(+) (pilD) [Pseudomonas aeruginosa strain 12939]
ATGACCCTCCTCGACTACCTGGCCAGCCATCCGCTGGCCTTTGTCTTGTGCGCCATCCTCCTTGGCCTGCTGGTCGGCAG
CTTCCTCAACGTCGTCGTGCATCGCCTGCCGAAGATGATGGAGCGCAACTGGAAGGCCGAGGCCCGCGAAGCGCTCGGGC
TGGAGCCGGAACCCAAGCAGGCGACCTACAACCTGGTGCTGCCGAATTCGGCGTGCCCGCGCTGCGGCCACGAGATCCGC
CCCTGGGAAAACATACCGCTGGTCAGCTACCTGGCGCTGGGCGGCAAGTGCTCGTCCTGCAAGGCCGCCATCGGCAAACG
CTACCCCCTGGTGGAACTGGCCACCGCGCTGCTTTCCGGCTATGTCGCCTGGCATTTCGGCTTCACCTGGCAGGCGGGCG
CGATGCTCCTGCTGACCTGGGGCCTGCTGGCGATGAGCCTGATCGATGCCGATCACCAACTGCTGCCGGACGTGCTGGTG
CTGCCGCTGCTCTGGCTGGGTCTGATCGCCAACCATTTCGGCCTCTTCGCCAGCCTCGACGATGCGCTGTTCGGCGCGGT
GTTCGGCTACCTGAGCCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACCGGCAAGGAGGGCATGGGCTACGGCGACT
TCAAGCTGCTGGCCATGCTCGGTGCCTGGGGTGGCTGGCAGATCCTGCCGCTGACCATCCTGCTGTCGTCGCTGGTCGGG
GCGATCCTCGGGGTGATCATGCTGCGCCTGCGCAACGCCGAAAGCGGCACGCCGATCCCCTTCGGTCCCTATCTGGCGAT
TGCCGGGTGGATTGCTTTGCTCTGGGGTGATCAAATAACCCGGACCTATCTGCAATTCGCCGGATTCAAATGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (137-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	55.714	96.552	0.538
pilD	Vibrio campbellii strain DS40M4	53.004	97.586	0.517
pilD	Acinetobacter nosocomialis M2	50.53	97.586	0.493
pilD	Acinetobacter baumannii D1279779	50.53	97.586	0.493
pilD	Neisseria gonorrhoeae MS11	49.281	95.862	0.472