Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NP7_RS09500 Genome accession   NZ_CP024443
Coordinates   2132380..2134086 (-) Length   568 a.a.
NCBI ID   WP_100270610.1    Uniprot ID   A0A2I5HRT2
Organism   Moraxella osloensis strain NP7     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2127380..2139086
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NP7_RS09480 (NP7_12810) - 2128102..2129508 (+) 1407 WP_100270607.1 nicotinate phosphoribosyltransferase -
  NP7_RS09485 (NP7_12815) coaE 2129566..2130222 (-) 657 WP_227523072.1 dephospho-CoA kinase -
  NP7_RS09490 (NP7_12820) pilD 2130219..2131094 (-) 876 WP_100270608.1 prepilin peptidase Machinery gene
  NP7_RS09495 (NP7_12825) pilC 2131100..2132323 (-) 1224 WP_100270609.1 type II secretion system F family protein Machinery gene
  NP7_RS09500 (NP7_12830) pilB 2132380..2134086 (-) 1707 WP_100270610.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NP7_RS09505 (NP7_12835) tpiA 2134521..2135375 (+) 855 WP_100270611.1 triose-phosphate isomerase -
  NP7_RS09510 (NP7_12840) secG 2135465..2135785 (+) 321 WP_062332397.1 preprotein translocase subunit SecG -
  NP7_RS09525 (NP7_12855) rimP 2136363..2136872 (+) 510 WP_100270612.1 ribosome maturation factor RimP -
  NP7_RS09530 (NP7_12860) nusA 2136990..2138471 (+) 1482 WP_100270613.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62593.14 Da        Isoelectric Point: 5.4517

>NTDB_id=253565 NP7_RS09500 WP_100270610.1 2132380..2134086(-) (pilB) [Moraxella osloensis strain NP7]
MAASLPSFGGIASRLVHEGLISEAAMQKVLAESQQHKVNLIAYVVDNKLAQADAVAWVISREFGDPLIDLDAINVNYIPK
EAVDEKIIREFNILPIFQRGSRLFVAMSDPTRVDALDALRFGTRFTVETVVAEHHKIKALIEKIFATNNLADFDDMDAVE
FDEARADEDSSATSHLDINDEAPVVKFVNGMLIKAITMGASDLHFEPYEKSYRVRFRIDGVLQKMANPPTQLAGKIAARL
KVMSQMDISERRVPQDGRIKLKISKDKAIDFRVNSLPTLFGEKIVLRILDPSSAMLGIDALGYEPDQKQLFMDALAKPQG
MLLITGPTGSGKTVSLYTGLNILNTVETNISTAEDPVEINLEGINQVNVNNKVGLTFASALKSFLRQDPDIVMVGEIRDL
ETAEIAVKAAQTGHMVLSTLHTNSAPETLTRLRNMGVASFNIATSVNLVIAQRLARRLCKACKKPTDVPKNSLLEMGFME
EDLANPSNVIYEPVGCAECRDGYKGRVGIYEVMKVTPEIARIIMEDGNAIEIKDAALKQGFRDLRRSGILKVLQGTTSIQ
EMYRVTSE

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=253565 NP7_RS09500 WP_100270610.1 2132380..2134086(-) (pilB) [Moraxella osloensis strain NP7]
ATGGCGGCATCCCTTCCTAGTTTTGGTGGCATTGCATCGCGATTAGTGCATGAAGGACTAATTAGCGAAGCGGCAATGCA
AAAAGTATTGGCAGAATCGCAGCAGCATAAAGTAAATTTAATCGCCTATGTGGTTGACAATAAGCTTGCGCAAGCTGACG
CGGTAGCGTGGGTGATTTCTCGAGAATTTGGCGACCCACTCATTGATTTGGATGCCATCAATGTCAACTATATTCCAAAA
GAAGCGGTTGATGAAAAAATTATTCGCGAATTTAATATTCTGCCGATTTTCCAACGAGGTAGCCGCTTATTTGTTGCCAT
GAGCGACCCGACGCGGGTAGATGCTTTAGACGCGCTGCGGTTTGGTACAAGGTTTACGGTAGAAACCGTGGTTGCCGAAC
ACCACAAAATCAAAGCACTCATCGAAAAAATCTTTGCTACCAACAATTTAGCTGATTTTGATGATATGGATGCGGTGGAG
TTTGATGAAGCCCGCGCCGATGAAGACAGTAGTGCCACCAGTCATTTAGATATCAATGATGAAGCACCTGTCGTCAAGTT
CGTTAATGGGATGCTCATCAAAGCCATTACCATGGGCGCCTCGGACTTACACTTTGAACCCTATGAAAAATCTTATCGCG
TGCGTTTTCGTATTGATGGGGTGTTGCAAAAGATGGCAAATCCACCGACGCAGCTAGCGGGTAAAATTGCTGCCCGCCTC
AAAGTCATGTCACAAATGGACATCTCTGAGCGCCGCGTGCCGCAAGATGGTCGTATCAAGTTAAAAATTTCCAAGGATAA
GGCGATTGATTTTCGTGTCAACTCATTGCCAACCTTATTTGGTGAAAAAATCGTACTGCGTATTTTAGACCCCTCATCAG
CGATGCTAGGCATTGATGCACTCGGCTATGAGCCGGACCAAAAGCAGCTATTTATGGATGCGCTGGCAAAACCGCAAGGG
ATGTTATTGATTACCGGTCCTACTGGTTCGGGTAAAACCGTATCCTTGTATACGGGGCTTAATATCTTAAACACAGTTGA
GACCAATATTTCCACCGCAGAAGATCCCGTTGAAATTAACCTTGAAGGCATCAATCAGGTCAACGTAAACAATAAAGTTG
GCTTGACCTTTGCCAGTGCGCTCAAATCATTCTTGCGTCAAGACCCTGATATTGTAATGGTCGGTGAGATTCGTGACTTG
GAAACCGCTGAAATTGCTGTCAAAGCTGCACAAACAGGGCATATGGTGCTGTCAACCTTGCATACCAACTCAGCGCCAGA
AACCTTAACGCGTCTGCGTAATATGGGGGTGGCATCCTTTAACATCGCCACCTCGGTCAATTTGGTAATCGCACAGCGCT
TAGCCCGTCGCCTGTGTAAAGCTTGTAAGAAACCGACAGATGTGCCAAAAAATAGCTTGCTTGAAATGGGCTTTATGGAA
GAAGATTTGGCAAATCCCAGCAATGTTATTTATGAGCCCGTTGGCTGCGCTGAATGCCGCGATGGCTATAAAGGACGTGT
TGGTATTTATGAAGTGATGAAAGTAACACCAGAGATTGCCCGAATTATTATGGAAGACGGCAACGCTATTGAGATTAAAG
ACGCGGCTTTAAAGCAAGGGTTTAGGGATTTAAGGCGCTCTGGCATTTTAAAGGTTCTACAAGGCACGACCAGTATCCAA
GAGATGTATCGTGTTACTAGCGAATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(15-146)

Type II/IV secretion system protein (T2SSE)

(191-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2I5HRT2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

68.021

99.648

0.678
  pilB Acinetobacter baylyi ADP1

65.432

99.824

0.653
  pilB Legionella pneumophila strain ERS1305867

50.973

99.472

0.507
  pilB Vibrio cholerae strain A1552

49.016

98.415

0.482
  pilF Neisseria gonorrhoeae MS11

49.016

98.415

0.482
  pilB Vibrio parahaemolyticus RIMD 2210633

47.1

100

0.472
  pilB Vibrio campbellii strain DS40M4

46.786

98.592

0.461
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.983

100

0.398
  pilF Thermus thermophilus HB27

36.824

100

0.371

Multiple sequence alignment