Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   YHS_RS09960 Genome accession   NZ_CP024176
Coordinates   2263780..2265486 (-) Length   568 a.a.
NCBI ID   WP_100274139.1    Uniprot ID   A0AAD0AXU8
Organism   Moraxella osloensis strain YHS     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2258780..2270486
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  YHS_RS09940 (YHS_11080) - 2259502..2260908 (+) 1407 WP_007115073.1 nicotinate phosphoribosyltransferase -
  YHS_RS09945 (YHS_11085) coaE 2260966..2261574 (-) 609 WP_100274136.1 dephospho-CoA kinase -
  YHS_RS09950 (YHS_11090) pilD 2261619..2262473 (-) 855 WP_157797036.1 prepilin peptidase Machinery gene
  YHS_RS09955 (YHS_11095) pilC 2262500..2263723 (-) 1224 WP_100274138.1 type II secretion system F family protein Machinery gene
  YHS_RS09960 (YHS_11100) pilB 2263780..2265486 (-) 1707 WP_100274139.1 type IV-A pilus assembly ATPase PilB Machinery gene
  YHS_RS09965 (YHS_11105) tpiA 2265909..2266763 (+) 855 WP_100274140.1 triose-phosphate isomerase -
  YHS_RS09970 (YHS_11110) secG 2266854..2267174 (+) 321 WP_062332397.1 preprotein translocase subunit SecG -
  YHS_RS09985 (YHS_11125) rimP 2267738..2268247 (+) 510 WP_100270612.1 ribosome maturation factor RimP -
  YHS_RS09990 (YHS_11130) nusA 2268365..2269846 (+) 1482 WP_100274141.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62549.96 Da        Isoelectric Point: 5.1457

>NTDB_id=252789 YHS_RS09960 WP_100274139.1 2263780..2265486(-) (pilB) [Moraxella osloensis strain YHS]
MAASLPGFGGIASRLVDEGLISEAAMQKVLAESQQQKINLIAYVVDNKLAQADAVAWVISREFGDPLIDLDAINVNYIPK
EAVDEKIIREFNIVPIFQRGSRLFVAMSDPTRVDALDALRFGTRFTVETVVAEHHKIKSLIEKIFASSNLADFDDMEAVE
FDETRADDDNSATSHLDVNDEAPVVKFVNGMLIKAITMGASDLHFEPYEKSYRVRFRIDGVLQKMANPPTQLAGKIAARL
KVMSQMDISERRVPQDGRIKLKISKDKAIDFRVNSLPTLFGEKIVLRILDPSSAMLGIDALGYEPDQKQLFMDALAKPQG
MLLITGPTGSGKTVSLYTGLNILNTVETNISTAEDPVEINLEGINQVNVNNKVGLTFASALKSFLRQDPDIVMVGEIRDL
ETAEIAVKAAQTGHMVLSTLHTNSAPETLTRLRNMGVASFNIATSVNLVIAQRLARRLCTACKKPTNVPKNSLLEMGFTE
EDLADPNNIIYEPVGCSECRDGYKGRVGIYEVMKVTPEIARIIMEDGNAIEIKDAALKQGFRDLRRSGILKVLKGTTSIQ
EMYRVTSE

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=252789 YHS_RS09960 WP_100274139.1 2263780..2265486(-) (pilB) [Moraxella osloensis strain YHS]
ATGGCAGCATCACTTCCTGGTTTTGGTGGCATTGCGTCACGATTAGTGGATGAAGGACTAATTAGCGAAGCAGCAATGCA
AAAAGTATTGGCAGAATCGCAGCAGCAAAAAATAAATTTAATCGCCTATGTGGTTGACAATAAATTGGCGCAAGCTGACG
CGGTAGCGTGGGTAATTTCGCGAGAATTTGGCGACCCACTCATTGATTTGGATGCCATCAATGTCAACTATATTCCAAAA
GAAGCGGTTGATGAAAAAATTATTCGCGAATTTAATATTGTGCCGATTTTCCAACGAGGTAGCCGCTTATTTGTTGCCAT
GAGTGACCCAACAAGGGTAGATGCTTTAGATGCGCTGCGATTTGGTACAAGGTTTACCGTAGAAACAGTGGTTGCCGAAC
ACCATAAAATAAAATCACTCATCGAAAAAATCTTTGCTAGCTCTAATTTAGCTGATTTTGATGATATGGAAGCGGTGGAG
TTTGATGAAACCCGCGCCGATGACGACAATAGTGCCACCAGTCATTTAGATGTCAATGATGAAGCGCCTGTCGTCAAGTT
CGTTAATGGGATGCTCATCAAAGCCATTACCATGGGCGCCTCAGACTTACACTTTGAACCCTATGAAAAATCTTATCGCG
TGCGTTTTCGTATTGATGGGGTGTTACAAAAAATGGCAAATCCACCGACGCAGCTAGCGGGTAAAATTGCTGCCCGCCTG
AAAGTCATGTCACAAATGGACATCTCTGAACGCCGCGTGCCGCAAGATGGTCGTATCAAGTTAAAAATTTCCAAGGATAA
GGCGATTGATTTTCGTGTCAACTCACTGCCAACCTTATTTGGTGAAAAAATCGTATTGCGTATTTTAGACCCATCATCAG
CGATGCTAGGTATTGATGCACTCGGCTATGAGCCTGACCAAAAGCAGCTATTTATGGATGCGCTGGCAAAACCGCAAGGG
ATGTTATTGATTACCGGCCCTACTGGTTCGGGTAAAACCGTCTCCTTGTACACCGGTCTTAATATCTTAAACACAGTTGA
GACCAATATTTCCACCGCAGAAGATCCCGTTGAAATTAACCTTGAAGGCATCAATCAGGTCAATGTGAACAATAAAGTTG
GCTTGACCTTTGCCAGTGCGCTCAAATCATTCTTGCGTCAAGACCCTGATATTGTGATGGTTGGTGAGATTCGTGACTTG
GAAACGGCTGAAATTGCTGTCAAAGCTGCCCAAACAGGGCATATGGTGCTGTCAACCTTGCATACCAATTCAGCGCCAGA
AACCTTAACGCGCCTGCGGAATATGGGGGTGGCATCCTTTAACATTGCCACCTCAGTCAATTTGGTAATCGCACAGCGCT
TAGCACGTCGCCTTTGTACAGCTTGTAAAAAACCGACCAATGTGCCAAAAAATAGCTTGCTTGAAATGGGCTTTACTGAA
GAAGATTTGGCAGATCCCAACAATATCATTTATGAGCCCGTTGGCTGCTCTGAATGCCGCGATGGCTATAAAGGACGTGT
TGGTATTTATGAAGTCATGAAAGTAACACCAGAGATTGCCCGAATTATTATGGAAGACGGCAATGCTATTGAGATTAAAG
ACGCTGCGTTAAAGCAAGGGTTTAGGGATTTAAGGCGCTCTGGCATTCTAAAGGTTCTAAAAGGCACGACAAGTATCCAA
GAGATGTATCGTGTTACTAGCGAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(15-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

67.668

99.648

0.674
  pilB Acinetobacter baylyi ADP1

65.426

99.296

0.65
  pilB Legionella pneumophila strain ERS1305867

51.767

99.648

0.516
  pilF Neisseria gonorrhoeae MS11

49.195

98.415

0.484
  pilB Vibrio cholerae strain A1552

49.275

97.183

0.479
  pilB Vibrio parahaemolyticus RIMD 2210633

47.247

99.12

0.468
  pilB Vibrio campbellii strain DS40M4

46.524

98.768

0.46
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.313

91.197

0.377
  pilF Thermus thermophilus HB27

36.475

100

0.368

Multiple sequence alignment