Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   CRN81_RS17815 Genome accession   NZ_CP024028
Coordinates   3820170..3821876 (-) Length   568 a.a.
NCBI ID   WP_011137375.1    Uniprot ID   Q7NRF4
Organism   Chromobacterium violaceum strain CV1192     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3815170..3826876
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CRN81_RS17790 (CRN81_17785) yacG 3816313..3816504 (-) 192 WP_011137370.1 DNA gyrase inhibitor YacG -
  CRN81_RS17795 (CRN81_17790) zapD 3816510..3817268 (-) 759 WP_011137371.1 cell division protein ZapD -
  CRN81_RS17800 (CRN81_17795) coaE 3817305..3817919 (-) 615 WP_099427576.1 dephospho-CoA kinase -
  CRN81_RS17805 (CRN81_17800) - 3817904..3818809 (-) 906 WP_196303056.1 prepilin peptidase -
  CRN81_RS17810 (CRN81_17805) pilC 3818821..3820044 (-) 1224 WP_011137374.1 type II secretion system F family protein Machinery gene
  CRN81_RS17815 (CRN81_17810) pilF 3820170..3821876 (-) 1707 WP_011137375.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CRN81_RS17820 (CRN81_17815) - 3822046..3823152 (-) 1107 WP_099427577.1 porin -
  CRN81_RS17825 (CRN81_17820) - 3823559..3823876 (-) 318 WP_011137378.1 ComEA family DNA-binding protein -
  CRN81_RS17830 (CRN81_17825) - 3823945..3824766 (-) 822 WP_099427578.1 inner membrane protein YpjD -
  CRN81_RS17835 (CRN81_17830) ffh 3824880..3826235 (+) 1356 WP_011137380.1 signal recognition particle protein -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62275.77 Da        Isoelectric Point: 5.8815

>NTDB_id=251310 CRN81_RS17815 WP_011137375.1 3820170..3821876(-) (pilF) [Chromobacterium violaceum strain CV1192]
MEATAGISGLGRALVQHNMLLQQDADAISKQATTSHLSFVEQLIQSKKMTSRDIAMFSSRVFGYPLLDLASLDTEQLPKG
LVDDEVIASRRLLPLLKRGNKLYIGTSDPTQTSAYHAITFKTGLTVEVVVVEEDKLSKLIGRYQDNSTSALKELESEDFG
DLEFADPDAQLDSGPQTEVDDAPVVKFIHKMLLDAIGIGASDIHFEPYEKYYRIRYRVDGVLKEVAQPPLLLKEKIASRI
KVISRLDISEKRLPQDGRLKLVISKTRAIDFRVSTLPTLYGEKIVMRILDSSGASLDIDQLGFEPEQKAHLLKAIARPYG
MVLVTGPTGSGKTVSLYTCLNILNKPDTNISTAEDPVEINLPGINQVNVNEKAGLTFASALKAFLRQDPDVIMVGEIRDF
ETADIAIKAAQTGHMVFSTLHTNDAPSTLTRMLNMGVAPFNIASSVLLIMAQRLARRLCSHCKQPVEIPKEALLRAGFTK
EDLAGGWHPYGPVGCEECRNTGYKGRVGIFEAMPISDAMMRLIMKNGTSVDISDLAKEEGMVDLRRAGLLKVMSGVTSLA
EVEAVTND

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=251310 CRN81_RS17815 WP_011137375.1 3820170..3821876(-) (pilF) [Chromobacterium violaceum strain CV1192]
ATGGAAGCTACGGCAGGGATTTCCGGCCTGGGCAGAGCCCTGGTTCAGCACAATATGCTATTGCAGCAAGATGCGGATGC
CATCTCAAAACAGGCGACGACGTCCCATCTCAGCTTCGTCGAGCAACTGATCCAGAGCAAGAAGATGACGTCGCGCGATA
TCGCGATGTTTTCATCGCGGGTTTTCGGCTATCCGCTGCTGGATTTGGCTTCGCTGGACACCGAACAATTGCCCAAGGGG
TTGGTGGACGACGAGGTGATCGCCAGCCGCCGCCTTTTGCCGTTGCTGAAGCGCGGCAACAAGCTGTATATCGGCACTTC
CGATCCTACCCAGACCTCGGCCTATCATGCGATCACCTTCAAGACCGGCTTGACGGTCGAGGTGGTGGTGGTGGAAGAGG
ATAAGCTGTCCAAGCTGATCGGCCGTTACCAGGACAATTCGACCTCGGCTTTGAAGGAGCTGGAGAGCGAGGACTTCGGC
GACCTGGAGTTCGCCGATCCGGACGCCCAGCTCGACAGCGGCCCGCAGACCGAGGTCGACGACGCGCCGGTGGTCAAGTT
CATCCACAAGATGCTGCTGGACGCGATAGGCATCGGCGCGTCCGACATCCACTTCGAACCTTACGAGAAATATTACCGCA
TCCGCTACCGGGTGGACGGCGTGCTGAAGGAGGTGGCGCAGCCGCCCTTGCTGCTGAAGGAAAAGATCGCCTCCCGCATC
AAGGTGATTTCCAGGCTGGACATTTCGGAAAAGCGCTTGCCGCAGGATGGCCGGTTGAAGTTGGTGATTTCCAAGACCCG
GGCGATAGATTTCCGCGTCAGCACGCTGCCCACGCTGTACGGCGAGAAGATCGTCATGCGTATCCTGGACTCGTCCGGCG
CCTCGCTGGATATCGACCAGCTGGGTTTCGAGCCGGAGCAGAAGGCGCATCTGCTGAAGGCGATCGCCAGGCCGTACGGC
ATGGTGCTGGTGACCGGTCCGACGGGCAGCGGCAAGACAGTGTCGCTGTACACCTGCCTGAACATCCTGAACAAGCCGGA
CACCAATATCTCCACGGCGGAGGATCCGGTTGAAATCAACCTTCCCGGCATCAACCAGGTCAACGTCAACGAGAAGGCCG
GGCTGACCTTCGCGTCGGCGCTGAAAGCCTTCTTGCGGCAGGATCCGGACGTGATCATGGTCGGCGAAATCCGCGATTTC
GAAACCGCCGACATCGCCATCAAGGCGGCTCAGACCGGCCACATGGTGTTTTCCACGCTGCATACCAATGACGCCCCGTC
CACGCTGACCCGGATGCTGAATATGGGCGTGGCGCCGTTCAACATCGCCAGCTCGGTGTTGCTGATCATGGCGCAGCGCC
TGGCGCGTCGCTTGTGCAGCCATTGCAAGCAGCCGGTGGAAATCCCGAAGGAAGCGCTGCTGCGCGCGGGTTTTACCAAA
GAGGACCTGGCGGGCGGCTGGCATCCGTACGGGCCGGTTGGCTGCGAGGAGTGCCGGAATACCGGCTACAAAGGCCGGGT
CGGCATTTTCGAAGCCATGCCGATCAGCGACGCGATGATGCGCTTGATCATGAAGAACGGCACCTCGGTCGACATCTCCG
ATCTGGCCAAGGAGGAGGGCATGGTCGATCTGCGCCGCGCCGGCCTGTTGAAGGTGATGAGCGGAGTGACTTCGCTGGCC
GAGGTCGAAGCGGTAACCAACGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q7NRF4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

58.541

98.944

0.579
  pilB Acinetobacter baumannii D1279779

55.308

100

0.569
  pilB Acinetobacter baylyi ADP1

54.529

99.12

0.54
  pilB Legionella pneumophila strain ERS1305867

53.054

100

0.535
  pilB Vibrio cholerae strain A1552

49.553

98.415

0.488
  pilB Vibrio parahaemolyticus RIMD 2210633

48.399

98.944

0.479
  pilB Vibrio campbellii strain DS40M4

46.359

99.12

0.46
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.299

94.366

0.38
  pilF Thermus thermophilus HB27

38.434

98.944

0.38

Multiple sequence alignment