Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CRN72_RS03580 Genome accession   NZ_CP023972
Coordinates   746758..748146 (-) Length   462 a.a.
NCBI ID   WP_096743230.1    Uniprot ID   -
Organism   Pasteurella multocida strain FDAARGOS_385     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 741758..753146
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CRN72_RS03550 (CRN72_03550) - 741885..743258 (-) 1374 WP_096743226.1 sodium:alanine symporter family protein -
  CRN72_RS03555 (CRN72_03555) - 743707..743976 (-) 270 WP_005722568.1 GNAT family N-acetyltransferase -
  CRN72_RS03560 (CRN72_03560) yacG 743976..744179 (-) 204 WP_083004817.1 DNA gyrase inhibitor YacG -
  CRN72_RS03565 (CRN72_03565) coaE 744190..744810 (-) 621 WP_096743227.1 dephospho-CoA kinase -
  CRN72_RS03570 (CRN72_03570) - 744822..745547 (-) 726 WP_096743228.1 A24 family peptidase -
  CRN72_RS03575 (CRN72_03575) pilC 745547..746758 (-) 1212 WP_096743229.1 type II secretion system F family protein Machinery gene
  CRN72_RS03580 (CRN72_03580) pilB 746758..748146 (-) 1389 WP_096743230.1 GspE/PulE family protein Machinery gene
  CRN72_RS03585 (CRN72_03585) pilA 748146..748580 (-) 435 WP_083004804.1 prepilin peptidase-dependent pilin Machinery gene
  CRN72_RS03590 (CRN72_03590) ampD 748740..749306 (+) 567 WP_083004802.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  CRN72_RS03595 (CRN72_03595) rppH 749892..750488 (+) 597 WP_083004799.1 RNA pyrophosphohydrolase -
  CRN72_RS03600 (CRN72_03600) - 750491..751285 (+) 795 WP_083004796.1 sulfite exporter TauE/SafE family protein -
  CRN72_RS03605 (CRN72_03605) lgt 751295..752098 (+) 804 WP_005751073.1 prolipoprotein diacylglyceryl transferase -
  CRN72_RS03610 (CRN72_03610) - 752104..752955 (+) 852 WP_083004791.1 thymidylate synthase -

Sequence

Protein


Download         Length: 462 a.a.        Molecular weight: 52161.81 Da        Isoelectric Point: 6.7142

>NTDB_id=251058 CRN72_RS03580 WP_096743230.1 746758..748146(-) (pilB) [Pasteurella multocida strain FDAARGOS_385]
MFHKNPLYTVSATTGEVFEIPQALWQKNQQQQRFLLRYFALPLQEDEKQLWLGVDSLANLSACDAFSFLTNKHIEPVLLS
KNELKRALQQLSPPKLQVEESIPTAYQVVQLDSDSATDEPLIQLLDQVFERALQLQASDIHFEPQQHSLQVRFRIDGVLQ
VQPSFSGILANRLISRLKLLAKLDISETRLPQDGCFQFKTTFSDTLDFRLSTLPTHLGEKAVLRLQRNKPIHLSFAELGM
TADQQYRFQHALSQPQGLILVTGPTGSGKSISLYSALAWLNSVEKHILTAEDPIEIALSGIIQTQVNTQIGLDFKRLLRT
FLRQDPDVIMLGEIRDEESALIALRAAQTGHLVLSTLHTNDAPSALTRLQQLGVPLHEIEQSLLLVVAQRLLRRRCCDCS
VETQSACRCYQGYKGRIGVYQFLQKDKTGCFVTDFSSLRESAKEKVRLGMTDDNEVQRVLGE

Nucleotide


Download         Length: 1389 bp        

>NTDB_id=251058 CRN72_RS03580 WP_096743230.1 746758..748146(-) (pilB) [Pasteurella multocida strain FDAARGOS_385]
ATGTTTCATAAGAATCCCTTATATACTGTTAGCGCGACGACAGGAGAGGTATTTGAAATCCCGCAGGCGCTATGGCAAAA
AAATCAGCAACAACAACGCTTTTTATTACGTTATTTTGCCTTGCCTTTACAAGAAGATGAAAAACAGCTGTGGCTTGGTG
TGGATTCGTTAGCGAATTTATCTGCATGTGATGCCTTTTCTTTTTTAACCAATAAACACATTGAGCCTGTTTTATTATCA
AAAAATGAACTTAAACGGGCATTGCAGCAACTTTCCCCACCAAAATTACAAGTAGAAGAAAGCATACCCACGGCTTATCA
AGTCGTGCAATTAGATTCTGATTCCGCAACGGATGAACCTTTGATTCAATTACTTGATCAGGTTTTTGAACGTGCATTAC
AGCTACAGGCTTCTGACATTCATTTTGAACCGCAACAGCATAGTTTACAAGTGCGTTTTAGAATCGATGGTGTATTACAA
GTCCAACCTTCCTTTTCAGGAATATTGGCTAATCGCCTGATTTCTCGTCTTAAATTATTAGCGAAATTAGATATTAGTGA
AACACGCTTGCCTCAAGACGGCTGTTTTCAATTTAAAACGACGTTTTCTGATACATTAGATTTTCGACTTTCTACGTTGC
CGACACATTTAGGCGAAAAAGCAGTATTACGTTTACAGCGAAATAAGCCTATTCATCTCAGTTTTGCTGAGTTGGGGATG
ACCGCAGACCAACAATACAGGTTCCAACACGCGTTAAGTCAGCCTCAAGGACTGATTTTAGTCACGGGACCAACAGGGAG
TGGAAAAAGCATTTCGCTTTACAGTGCGTTAGCTTGGCTCAATAGCGTAGAAAAGCATATTTTAACAGCAGAAGATCCGA
TTGAAATTGCGTTGAGCGGGATAATCCAAACGCAAGTCAATACGCAAATAGGACTGGATTTCAAGCGCTTATTACGCACT
TTTTTACGTCAAGATCCAGACGTTATTATGTTAGGGGAAATTCGTGATGAGGAAAGTGCGTTAATCGCATTACGTGCAGC
TCAAACTGGGCATTTAGTCCTCTCTACCTTGCATACGAATGATGCCCCCTCTGCCCTTACTCGCTTACAGCAATTAGGGG
TGCCTTTGCATGAAATTGAACAAAGCCTATTACTTGTTGTGGCGCAGCGTTTATTGCGTCGTCGTTGTTGTGATTGCAGT
GTAGAGACACAATCGGCGTGTCGTTGCTATCAAGGGTATAAAGGGAGGATTGGGGTATATCAATTTTTACAAAAAGATAA
AACAGGGTGTTTTGTCACGGATTTTAGTAGTTTACGTGAGAGTGCGAAGGAAAAAGTTCGTTTAGGCATGACTGATGACA
ATGAAGTACAACGTGTATTAGGTGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(128-394)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(16-93)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

67.991

98.052

0.667
  pilB Haemophilus influenzae Rd KW20

67.329

98.052

0.66
  pilB Glaesserella parasuis strain SC1401

58.134

99.784

0.58
  pilB Vibrio parahaemolyticus RIMD 2210633

40.541

100

0.422
  pilB Vibrio campbellii strain DS40M4

39.834

100

0.416
  pilB Acinetobacter baumannii D1279779

38.98

100

0.413
  pilB Vibrio cholerae strain A1552

39.095

100

0.411
  pilB Acinetobacter baylyi ADP1

37.675

100

0.407
  pilB Legionella pneumophila strain ERS1305867

36.605

100

0.387

Multiple sequence alignment