Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   CRN72_RS03585 Genome accession   NZ_CP023972
Coordinates   748146..748580 (-) Length   144 a.a.
NCBI ID   WP_083004804.1    Uniprot ID   A0A1Q1PRS7
Organism   Pasteurella multocida strain FDAARGOS_385     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 743146..753580
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CRN72_RS03555 (CRN72_03555) - 743707..743976 (-) 270 WP_005722568.1 GNAT family N-acetyltransferase -
  CRN72_RS03560 (CRN72_03560) yacG 743976..744179 (-) 204 WP_083004817.1 DNA gyrase inhibitor YacG -
  CRN72_RS03565 (CRN72_03565) coaE 744190..744810 (-) 621 WP_096743227.1 dephospho-CoA kinase -
  CRN72_RS03570 (CRN72_03570) - 744822..745547 (-) 726 WP_096743228.1 A24 family peptidase -
  CRN72_RS03575 (CRN72_03575) pilC 745547..746758 (-) 1212 WP_096743229.1 type II secretion system F family protein Machinery gene
  CRN72_RS03580 (CRN72_03580) pilB 746758..748146 (-) 1389 WP_096743230.1 GspE/PulE family protein Machinery gene
  CRN72_RS03585 (CRN72_03585) pilA 748146..748580 (-) 435 WP_083004804.1 prepilin peptidase-dependent pilin Machinery gene
  CRN72_RS03590 (CRN72_03590) ampD 748740..749306 (+) 567 WP_083004802.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  CRN72_RS03595 (CRN72_03595) rppH 749892..750488 (+) 597 WP_083004799.1 RNA pyrophosphohydrolase -
  CRN72_RS03600 (CRN72_03600) - 750491..751285 (+) 795 WP_083004796.1 sulfite exporter TauE/SafE family protein -
  CRN72_RS03605 (CRN72_03605) lgt 751295..752098 (+) 804 WP_005751073.1 prolipoprotein diacylglyceryl transferase -
  CRN72_RS03610 (CRN72_03610) - 752104..752955 (+) 852 WP_083004791.1 thymidylate synthase -
  CRN72_RS03615 (CRN72_03615) tadA 752982..753470 (+) 489 WP_083004788.1 tRNA adenosine(34) deaminase TadA -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15072.46 Da        Isoelectric Point: 8.8040

>NTDB_id=251059 CRN72_RS03585 WP_083004804.1 748146..748580(-) (pilA) [Pasteurella multocida strain FDAARGOS_385]
MKKAIFFSIQKGFTLIELMIVIAIIAILATIAVPSYQNYTKKAAISELLQAAAPYRAEVELCIYNTNSTTNCNAGSHGIR
ADAAASKKYLKAITVKAGVITVTGQGSLEGVSYTLTASGNAVQGVSWTVNCGAQSDLFPAGFCA

Nucleotide


Download         Length: 435 bp        

>NTDB_id=251059 CRN72_RS03585 WP_083004804.1 748146..748580(-) (pilA) [Pasteurella multocida strain FDAARGOS_385]
ATGAAAAAAGCCATTTTCTTTTCTATTCAAAAAGGGTTTACCCTCATTGAATTAATGATTGTCATTGCCATTATTGCGAT
TTTAGCCACGATTGCCGTGCCATCTTATCAAAATTATACAAAAAAAGCGGCAATCTCTGAATTATTGCAAGCCGCTGCTC
CTTATCGTGCCGAGGTAGAACTTTGTATCTATAATACAAATAGTACAACGAATTGTAATGCAGGAAGTCATGGTATTCGT
GCTGATGCGGCAGCAAGTAAAAAGTATTTAAAAGCCATCACGGTTAAAGCAGGGGTAATTACTGTTACTGGACAAGGGAG
TTTGGAAGGAGTTAGCTATACGCTGACAGCGAGTGGTAACGCAGTTCAAGGTGTTTCTTGGACCGTTAATTGTGGTGCTC
AATCTGACCTTTTCCCTGCAGGATTTTGTGCATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)

Pilin (bacterial filament) (Pilin)

(42-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1Q1PRS7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

62.5

100

0.625
  pilA Haemophilus influenzae Rd KW20

60.417

100

0.604
  pilA Glaesserella parasuis strain SC1401

53.02

100

0.549
  pilA Vibrio campbellii strain DS40M4

34.437

100

0.361

Multiple sequence alignment