Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   PFL01_RS02065 Genome accession   NC_007492
Coordinates   464468..466546 (+) Length   692 a.a.
NCBI ID   WP_011332080.1    Uniprot ID   Q3KJA3
Organism   Pseudomonas fluorescens Pf0-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 459468..471546
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PFL01_RS02045 (Pfl01_0405) comM 461682..462746 (+) 1065 WP_011332076.1 pilus assembly protein PilM Machinery gene
  PFL01_RS02050 (Pfl01_0406) - 462746..463312 (+) 567 WP_011332077.1 PilN domain-containing protein -
  PFL01_RS02055 (Pfl01_0407) pilO 463309..463932 (+) 624 WP_011332078.1 type 4a pilus biogenesis protein PilO -
  PFL01_RS02060 (Pfl01_0408) - 463929..464453 (+) 525 WP_011332079.1 pilus assembly protein PilP -
  PFL01_RS02065 (Pfl01_0409) pilQ 464468..466546 (+) 2079 WP_011332080.1 type IV pilus secretin PilQ Machinery gene
  PFL01_RS02070 (Pfl01_0410) aroK 466551..467069 (+) 519 WP_011332081.1 shikimate kinase AroK -
  PFL01_RS02075 (Pfl01_0411) aroB 467222..468322 (+) 1101 WP_011332082.1 3-dehydroquinate synthase -
  PFL01_RS02080 (Pfl01_0412) - 468333..469934 (+) 1602 WP_011332083.1 AAA family ATPase -

Sequence

Protein


Download         Length: 692 a.a.        Molecular weight: 75023.13 Da        Isoelectric Point: 5.5724

>NTDB_id=24828 PFL01_RS02065 WP_011332080.1 464468..466546(+) (pilQ) [Pseudomonas fluorescens Pf0-1]
MNRIFSTLGFSLWIALLSPMVQAANLKALDVAALPGDRVELKLSFDGPPPQPKGYTTDSPARIALDLPGVASQLASKTRD
LGSGNARTATVVEANNRTRLIISLTQLAPYDTRVEGNTVFVVVGQGSKAAAPRTAARAPRAAPAAAPVRTYTPTARAIRG
VDFQRGTAGEGNVVIDLSDPAIAPDIQEHDGKIILNFARTQLPEPLRVRLDVKDFATPVQFVNAGVSGDRATITVEPSGT
FDYSTYQTDNKLTVSIRPLTVDDLQKRNADRNSYNGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQNV
PWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADIAKLFQSVTSAEAK
VDERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNKGNWNTSGVSN
GSSTTIGTPGSTSTNSPFVDMGTVNNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPY
QEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFSN
TQSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2079 bp        

>NTDB_id=24828 PFL01_RS02065 WP_011332080.1 464468..466546(+) (pilQ) [Pseudomonas fluorescens Pf0-1]
ATGAACAGGATTTTCTCCACCCTCGGTTTCTCGCTATGGATAGCGCTGCTTTCGCCGATGGTACAGGCAGCCAATCTGAA
GGCGCTGGACGTCGCGGCACTGCCGGGCGACCGGGTCGAGCTGAAGCTGTCGTTCGATGGCCCACCTCCGCAGCCAAAGG
GTTACACCACCGATTCGCCGGCGCGGATTGCCCTCGATCTGCCGGGTGTTGCCAGTCAGCTGGCGAGCAAGACTCGCGAC
CTGGGCAGTGGCAATGCCCGTACGGCCACGGTGGTCGAGGCCAATAATCGGACGCGGCTGATCATCAGCCTGACGCAACT
GGCGCCGTACGACACCCGGGTCGAAGGCAATACGGTGTTTGTGGTGGTCGGGCAGGGCAGCAAAGCCGCGGCACCGCGTA
CGGCAGCTCGTGCACCGCGTGCGGCACCTGCGGCAGCGCCGGTCCGCACGTATACGCCGACCGCCAGGGCCATTCGCGGC
GTGGATTTCCAGCGTGGCACGGCGGGTGAGGGCAATGTGGTCATCGACTTGTCCGACCCGGCTATCGCCCCGGACATTCA
GGAGCACGACGGCAAGATCATCCTCAATTTCGCCCGTACCCAACTGCCCGAACCGCTGCGGGTGCGCCTCGATGTCAAGG
ATTTCGCCACCCCGGTGCAGTTCGTCAATGCCGGCGTCAGTGGTGATCGCGCGACGATCACCGTCGAACCCAGCGGTACG
TTCGACTATTCCACCTACCAGACCGACAACAAGCTGACCGTCAGTATCCGGCCGTTGACCGTCGATGACCTGCAAAAGCG
CAACGCCGACCGCAACAGCTATAACGGCGAAAAGCTCTCTCTCAACTTCCAGGACATCGATGTGCGTTCGGTGCTGCAAC
TGATCGCCGACTTCACCAACCTCAATCTGGTAGCCAGCGATACGGTACAGGGCGGGATCACCTTGCGCCTGCAAAACGTG
CCGTGGGATCAGGCGCTGGATCTGGTGCTCAAGACCAAGGGGCTGGACAAGCGCAAGGTCGGCAATGTGTTGCTGGTGGC
GCCGGCGGATGAAATCGCCGCCCGCGAACGGCAGGAACTGGAATCGCAAAAACAGATCGCCGAGCTGGCACCGCTGCGCC
GCGAGCTGCTGCAAGTGAACTACGCCAAGGCTGCGGACATCGCCAAGCTGTTCCAGTCGGTCACCAGTGCCGAGGCCAAG
GTCGACGAACGGGGTTCGATCACCGTCGATGAGCGGACCAACAACATCATTGCCTACCAGACTCAGGATCGCCTCGACGA
ACTGCGGCGGATTGTGGCGCAGCTGGATATTCCGGTGCGTCAGGTGATGATCGAGGCGCGGATCGTCGAGGCCAACGTCG
ATTACGACAAGAGCTTGGGCGTGCGCTGGGGCGGTTCGATTCAGAACAAGGGCAACTGGAACACTTCCGGGGTCAGCAAC
GGTTCGTCGACCACCATTGGCACGCCGGGCAGCACCAGCACCAACTCGCCGTTCGTCGACATGGGCACGGTCAACAACAC
GTCCGGGATCGGCATCGCGTTCATCACCGACAACGTCTTGCTCGACCTTGAGCTGACCGCGATGGAGAAGACCGGCAATG
GCGAAATCGTCTCGCAGCCCAAGGTGGTCACGTCCGACAAGGAAACCGCGAAGATCCTCAAGGGCACCGAGATTCCGTAT
CAGGAAGCCAGCTCCAGCGGTGCTACGTCGGTGTCCTTCAAGGAGGCGTCGCTGTCGCTGGAAGTGACGCCGCAGATCAC
CCCGGACAACCGCATCATCATGGAGGTCAAGGTCACCAAGGACGAACCGGACTACCTGAACAAAGTGCAGGATGTACCGC
CGATCAAGAAGAACGAGGTCAATGCCAAGGTGCTGGTGAACGACGGCGAGACCATCGTGATCGGGGGCGTTTTCTCAAAT
ACTCAAAGCAAGGTCGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATGTGGT
TTCGGAGAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(376-441)

Bacterial type II and III secretion system protein (Secretin)

(527-683)

AMIN domain (AMIN)

(37-121)

Secretin and TonB N terminus short domain (STN)

(302-348)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3KJA3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

71.827

100

0.744
  pilQ Acinetobacter baumannii strain A118

37.784

100

0.409
  pilQ Acinetobacter baumannii D1279779

37.784

100

0.409
  comQ Acinetobacter baylyi ADP1

40.778

100

0.409
  pilQ Legionella pneumophila strain Lp02

37.63

97.543

0.367
  pilQ Legionella pneumophila strain ERS1305867

37.593

97.254

0.366

Multiple sequence alignment