Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   PFL01_RS02045 Genome accession   NC_007492
Coordinates   461682..462746 (+) Length   354 a.a.
NCBI ID   WP_011332076.1    Uniprot ID   Q3KJA7
Organism   Pseudomonas fluorescens Pf0-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 456682..467746
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PFL01_RS02035 (Pfl01_0403) - 457553..458821 (+) 1269 WP_007952395.1 malic enzyme-like NAD(P)-binding protein -
  PFL01_RS02040 (Pfl01_0404) - 459017..461464 (-) 2448 WP_011332075.1 penicillin-binding protein 1A -
  PFL01_RS02045 (Pfl01_0405) comM 461682..462746 (+) 1065 WP_011332076.1 pilus assembly protein PilM Machinery gene
  PFL01_RS02050 (Pfl01_0406) - 462746..463312 (+) 567 WP_011332077.1 PilN domain-containing protein -
  PFL01_RS02055 (Pfl01_0407) pilO 463309..463932 (+) 624 WP_011332078.1 type 4a pilus biogenesis protein PilO -
  PFL01_RS02060 (Pfl01_0408) - 463929..464453 (+) 525 WP_011332079.1 pilus assembly protein PilP -
  PFL01_RS02065 (Pfl01_0409) pilQ 464468..466546 (+) 2079 WP_011332080.1 type IV pilus secretin PilQ Machinery gene
  PFL01_RS02070 (Pfl01_0410) aroK 466551..467069 (+) 519 WP_011332081.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38147.66 Da        Isoelectric Point: 4.7539

>NTDB_id=24827 PFL01_RS02045 WP_011332076.1 461682..462746(+) (comM) [Pseudomonas fluorescens Pf0-1]
MLGLFNKKANTLLGIDISSTSVKLLELSRQGERYRVEAYAVEPLPANAVVEKNIAELEGVGQALSRVLVKARTGLKTVAV
AVAGSAVITKVIEMDAGLSDDELENQLKIEADQYIPYPLDEVAIDFEVQGVSPRNPERVNVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLATQLAASQDRLTVAVVDIGATMTTLSVLHNGRIIYTREQLFGGRQLTEEIQRRYGLTL
EQAGLAKKQGGLPDDYVSEVLQPFREALVQQVSRSLQFFFASGQYNAVDHILLAGGTASVPGLDRLIEERLHTPTQVANP
FSDMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=24827 PFL01_RS02045 WP_011332076.1 461682..462746(+) (comM) [Pseudomonas fluorescens Pf0-1]
GTGCTAGGACTCTTCAATAAAAAGGCCAATACGTTACTGGGGATCGACATCAGCTCCACATCGGTCAAGCTGCTGGAGCT
GAGCCGTCAGGGTGAGCGCTACCGGGTCGAGGCGTACGCGGTGGAGCCGTTGCCGGCGAACGCCGTGGTCGAAAAGAACA
TCGCCGAGCTCGAGGGCGTTGGCCAGGCTCTGTCCCGGGTGCTGGTCAAGGCGCGCACCGGGCTGAAAACCGTGGCGGTG
GCGGTGGCTGGATCTGCGGTGATCACCAAGGTCATCGAGATGGACGCCGGGCTGTCCGACGACGAGCTGGAAAACCAGCT
CAAGATCGAAGCCGATCAATACATTCCCTATCCGCTGGACGAGGTCGCCATCGACTTCGAAGTCCAGGGCGTTTCGCCGC
GCAACCCCGAACGGGTCAACGTGCTGCTGGCCGCCTGTCGCAAGGAAAACGTCGAAGTCCGCGAAGCGGCGCTCGCTCTC
GCCGGGCTGACCGCGCGGGTGGTGGATGTAGAGGCGTATGCGCTGGAGCGCTCGTTCGGCCTGCTGGCCACGCAACTGGC
CGCGTCCCAGGATCGGTTGACGGTGGCGGTGGTCGACATCGGCGCCACCATGACCACCCTCAGCGTCCTGCACAACGGCC
GGATCATCTACACCCGCGAACAACTGTTCGGCGGTCGACAGCTGACCGAGGAAATCCAGCGCCGTTACGGCCTGACCCTT
GAGCAGGCGGGGCTCGCCAAAAAGCAGGGCGGCCTGCCGGACGACTACGTCAGTGAGGTGCTGCAGCCATTTCGCGAGGC
GCTGGTACAGCAGGTGTCGCGCTCCCTGCAGTTCTTTTTCGCCTCCGGCCAGTACAACGCTGTCGACCACATTCTGCTGG
CCGGCGGCACGGCGTCGGTGCCCGGCCTTGACCGGCTGATCGAAGAGCGCCTGCACACCCCGACCCAGGTTGCCAACCCG
TTTTCCGACATGGCCTTGAGCAGCAAGGTCAACGCCGGGGCCCTGGCGAGCGATGCGCCGGCCCTGATGATTGCCTGCGG
GCTCGCGCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3KJA7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Acinetobacter nosocomialis M2

56.215

100

0.562
  pilM Acinetobacter baumannii D1279779

56.215

100

0.562
  comM Acinetobacter baylyi ADP1

54.237

100

0.542
  pilM Legionella pneumophila strain ERS1305867

49.296

100

0.494

Multiple sequence alignment