Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PSHA_RS01875 Genome accession   NC_007481
Coordinates   393375..395048 (-) Length   557 a.a.
NCBI ID   WP_011327089.1    Uniprot ID   -
Organism   Pseudoalteromonas translucida     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 388375..400048
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSHA_RS01850 (PSHAa0373) - 389162..390094 (-) 933 WP_011327084.1 alpha/beta fold hydrolase -
  PSHA_RS01855 (PSHAa0374) - 390098..391513 (-) 1416 WP_041454327.1 ATP-binding protein -
  PSHA_RS01860 (PSHAa0375) - 391491..392180 (-) 690 WP_011327086.1 response regulator transcription factor -
  PSHA_RS01865 (PSHAa0376) - 392345..392830 (+) 486 WP_011327087.1 Spy/CpxP family protein refolding chaperone -
  PSHA_RS01870 (PSHAa0377) yacG 392989..393219 (-) 231 WP_011327088.1 DNA gyrase inhibitor YacG -
  PSHA_RS01875 (PSHAa0378) pilB 393375..395048 (-) 1674 WP_011327089.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PSHA_RS01880 (PSHAa0379) coaE 395169..395801 (-) 633 WP_011327090.1 dephospho-CoA kinase -
  PSHA_RS01885 (PSHAa0380) pilD 395803..396699 (-) 897 WP_041454328.1 A24 family peptidase Machinery gene
  PSHA_RS01890 (PSHAa0381) pilC 396727..397953 (-) 1227 WP_011327092.1 type II secretion system F family protein Machinery gene
  PSHA_RS01895 (PSHAa0382) - 398078..398689 (+) 612 WP_011327093.1 hypothetical protein -
  PSHA_RS01900 (PSHAa0383) - 398670..399101 (-) 432 WP_041454329.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  PSHA_RS01905 (PSHAa0384) - 399118..399549 (-) 432 WP_011327095.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -

Sequence

Protein


Download         Length: 557 a.a.        Molecular weight: 62055.61 Da        Isoelectric Point: 6.2140

>NTDB_id=24786 PSHA_RS01875 WP_011327089.1 393375..395048(-) (pilB) [Pseudoalteromonas translucida]
MNINSPLLRKFITLGRVDADIVKTKQQEFASTAELIAKCGKIDSKDLAAQCIDLFRVPFFDLKDFNTALISPELIKEKLI
RKHHILPLVQKGRKVYIAASDPTDYGAFENFEFSTGLSCEVVVVDYIQLDKKIEQLLDATGSLNLSDDEFKEFAELDADI
QKDTTKDDDKDDAPIIVYINKILMDAIKKGASDLHFEPYEHKYRIRFRIDGILHEVASPPNSLATKLSARIKVMSRLDIA
EKRKPQDGRIKLKITERKSVDFRVSTMPTLWGEKIVMRILDSSSAMLGIDVLGYEPEQKKLYMDALEQPQGMILVTGPTG
SGKTVSLYTGLNILNQPERNISTAEDPVEINLEGVNQVQINIKADMTFANALRAFLRQDPDVVMVGEIRDLETAEISIKA
AQTGHLVLSTLHTNSAPETITRLLNMGVPAYNVASSISLIIAQRLARRLCPKCKTPENLPSEELLRQGFSQQQISEMTLY
APKGCENCTDGYKGRVGIYEVMKITPEIAQIIMRGGNSLEIAEVSLKAGFNNLRLSGIRKAADGMTSLAEINRVTSF

Nucleotide


Download         Length: 1674 bp        

>NTDB_id=24786 PSHA_RS01875 WP_011327089.1 393375..395048(-) (pilB) [Pseudoalteromonas translucida]
ATGAATATTAACTCACCGTTATTAAGAAAATTTATTACCCTTGGCCGTGTTGATGCCGATATAGTAAAAACTAAACAGCA
AGAGTTTGCCTCTACTGCTGAGCTTATTGCTAAATGCGGAAAAATTGACAGTAAAGATTTAGCGGCGCAATGCATTGATT
TATTTAGGGTGCCTTTTTTTGATTTAAAAGACTTTAATACTGCACTTATTTCTCCTGAGTTAATAAAAGAAAAACTGATC
CGTAAGCATCATATTTTGCCTTTAGTACAAAAAGGCAGAAAAGTGTATATTGCCGCCTCTGATCCTACCGATTACGGCGC
ATTTGAAAACTTTGAATTTAGTACTGGTTTGTCTTGCGAAGTGGTAGTGGTTGACTACATTCAACTCGATAAAAAAATAG
AACAATTGCTTGATGCCACTGGCAGCTTAAATTTAAGTGATGACGAGTTTAAAGAGTTTGCCGAACTAGACGCCGATATA
CAAAAAGACACCACTAAAGACGATGACAAAGACGATGCGCCAATCATTGTTTACATCAATAAAATATTAATGGATGCGAT
TAAAAAAGGTGCGTCTGATTTACACTTTGAACCCTACGAGCACAAGTACCGAATTCGTTTTAGAATTGACGGTATTTTGC
ACGAAGTAGCCAGCCCACCAAACAGTTTAGCTACTAAGCTTTCGGCGCGTATTAAAGTAATGTCGCGCCTTGATATTGCC
GAAAAACGTAAACCACAAGATGGCCGCATAAAGCTAAAAATAACCGAGCGTAAAAGTGTCGATTTTCGGGTAAGCACCAT
GCCCACTTTATGGGGCGAAAAGATTGTAATGCGTATTCTTGATTCATCTAGCGCCATGCTTGGCATTGATGTATTAGGTT
ACGAACCCGAGCAAAAAAAGCTGTATATGGATGCGCTTGAGCAACCACAAGGGATGATATTAGTAACTGGGCCTACTGGC
TCGGGTAAAACGGTATCGCTTTACACCGGCTTAAATATATTAAACCAGCCTGAGCGTAATATTAGTACCGCCGAAGATCC
GGTAGAAATTAACCTCGAAGGGGTTAACCAAGTACAAATTAATATTAAAGCCGATATGACCTTTGCCAACGCATTGCGCG
CATTTTTACGCCAAGATCCCGATGTAGTAATGGTAGGTGAAATACGTGACCTAGAAACCGCCGAAATATCAATTAAAGCG
GCGCAAACAGGTCACTTAGTGCTTTCGACCTTGCATACTAACTCGGCGCCCGAAACCATTACCCGTTTATTAAACATGGG
CGTACCGGCATATAACGTAGCAAGTTCAATTAGTTTAATTATAGCTCAGCGCCTAGCGCGCCGCTTATGCCCTAAATGTA
AAACACCTGAAAATTTACCTAGCGAAGAACTGCTACGCCAAGGCTTTAGCCAGCAACAAATTAGTGAAATGACCCTGTAT
GCACCTAAAGGTTGCGAAAACTGTACCGATGGCTATAAAGGTCGTGTAGGTATTTACGAAGTAATGAAAATTACCCCAGA
AATAGCACAAATTATAATGCGCGGTGGTAACTCACTAGAAATAGCTGAAGTGTCGCTTAAAGCCGGATTTAATAACCTGC
GTTTATCGGGTATTCGTAAAGCCGCCGACGGCATGACCTCGCTAGCCGAGATTAATCGGGTAACGAGTTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(181-448)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

59.109

92.639

0.548
  pilB Acinetobacter baumannii D1279779

58.541

93.537

0.548
  pilB Legionella pneumophila strain ERS1305867

51.743

97.846

0.506
  pilB Vibrio cholerae strain A1552

52.158

95.691

0.499
  pilB Vibrio parahaemolyticus RIMD 2210633

51.97

95.691

0.497
  pilB Vibrio campbellii strain DS40M4

51.311

95.871

0.492
  pilF Neisseria gonorrhoeae MS11

49.712

93.537

0.465
  pilB/pilB1 Synechocystis sp. PCC 6803

37.359

100

0.417
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.77

99.82

0.377

Multiple sequence alignment