Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   CNN82_RS28110 Genome accession   NZ_CP023466
Coordinates   6127927..6130002 (-) Length   691 a.a.
NCBI ID   WP_096481367.1    Uniprot ID   A0AB33EID3
Organism   Pseudomonas frederiksbergensis strain KNU-15     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6122927..6135002
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CNN82_RS28095 (CNN82_28095) - 6124529..6126142 (-) 1614 WP_096481365.1 AAA family ATPase -
  CNN82_RS28100 (CNN82_28100) aroB 6126153..6127253 (-) 1101 WP_019581861.1 3-dehydroquinate synthase -
  CNN82_RS28105 (CNN82_28105) aroK 6127404..6127922 (-) 519 WP_019581860.1 shikimate kinase AroK -
  CNN82_RS28110 (CNN82_28110) pilQ 6127927..6130002 (-) 2076 WP_096481367.1 type IV pilus secretin PilQ Machinery gene
  CNN82_RS28115 (CNN82_28115) - 6130016..6130543 (-) 528 WP_019581858.1 pilus assembly protein PilP -
  CNN82_RS28120 (CNN82_28120) pilO 6130540..6131163 (-) 624 WP_019581857.1 type 4a pilus biogenesis protein PilO -
  CNN82_RS28125 (CNN82_28125) - 6131160..6131726 (-) 567 WP_019581856.1 PilN domain-containing protein -
  CNN82_RS28130 (CNN82_28130) comM 6131726..6132790 (-) 1065 WP_019581855.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 691 a.a.        Molecular weight: 74761.92 Da        Isoelectric Point: 5.7805

>NTDB_id=247275 CNN82_RS28110 WP_096481367.1 6127927..6130002(-) (pilQ) [Pseudomonas frederiksbergensis strain KNU-15]
MNRIFSTFGISLWIAFLSPLVQAANLKALDVAALPGDRVELKLSFDGPPPQPHGYTTEQPARIALDLPGVTSQLANKSRD
LGGGNARSATVVEAKDRTRLIIGLTQLTPYNARVEGNNLFVVVGQAAGNKASKPIASAPRAATAVPAPARASAPVSKAIR
GVDFQRGTQGEGNVVIDLSDPSIAPDIQERDGKIIVGFTKTQLPERLRVRLDVKDFATPVQFVNASATGDRAIISIEPSG
AFDYSTYQTDNKLTVSIRPMTVDDLQKRNAERFAYSGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQS
VPWDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADIAKLFQSVTSAEA
KVDERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSVQKGNWNGSGVNG
SSTTVGTPGSTSTNSPFVDLGTSSNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPYQ
EASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFSNT
QSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSH

Nucleotide


Download         Length: 2076 bp        

>NTDB_id=247275 CNN82_RS28110 WP_096481367.1 6127927..6130002(-) (pilQ) [Pseudomonas frederiksbergensis strain KNU-15]
ATGAACAGGATTTTCTCAACCTTCGGTATTTCGCTATGGATAGCGTTTCTGTCGCCGTTGGTACAGGCGGCCAACCTCAA
AGCGCTGGATGTCGCCGCGCTGCCTGGTGACCGTGTCGAGCTGAAGTTGTCGTTCGACGGGCCACCACCGCAGCCGCATG
GCTATACGACGGAGCAGCCTGCGCGGATTGCGCTGGACCTGCCGGGGGTTACCAGCCAGCTGGCGAACAAAAGTCGTGAC
CTGGGCGGCGGCAATGCCCGCAGCGCCACGGTGGTGGAAGCCAAGGACCGCACGCGGCTGATCATCGGTCTGACTCAACT
GACTCCCTATAACGCACGTGTCGAAGGCAACAACCTGTTTGTGGTTGTCGGGCAGGCGGCCGGTAACAAGGCGTCCAAAC
CGATTGCCAGCGCACCACGCGCGGCCACGGCGGTACCCGCGCCCGCCAGAGCCAGTGCTCCCGTGAGCAAGGCTATTCGT
GGCGTGGATTTCCAGCGCGGTACCCAGGGTGAAGGCAACGTGGTTATCGATTTGTCGGATCCGTCCATCGCCCCGGACAT
CCAGGAACGCGATGGCAAGATCATCGTCGGCTTCACCAAAACCCAATTGCCTGAGCGGTTGCGCGTCCGTCTCGACGTCA
AGGATTTCGCTACCCCGGTGCAGTTCGTCAACGCCAGCGCCACGGGCGACCGGGCCATCATCAGCATCGAGCCCAGCGGC
GCCTTCGATTATTCGACCTACCAGACCGATAACAAACTGACCGTCAGCATCCGGCCGATGACTGTCGATGACTTGCAAAA
ACGCAACGCCGAACGTTTTGCCTACAGCGGCGAAAAGCTCTCGTTGAATTTCCAGGACATCGATGTGCGCTCGGTGCTGC
AACTGATCGCCGATTTCACCAACCTCAATCTGGTGGCCAGTGACACGGTGCAGGGCGGCATCACGTTGCGCCTGCAGAGT
GTGCCGTGGGATCAGGCGCTGGACCTGGTGTTGAAAACCAAAGGCCTGGATAAACGCAAGATTGGCAATGTGTTGCTGGT
GGCGCCGGCCGATGAAATCGCCGCCCGGGAGCGTCAGGAGCTGGAGTCGCAGAAGCAAATCGCCGAACTGGCGCCTCTGC
GTCGCGAACTGTTGCAGGTCAACTACGCCAAGGCAGCCGATATCGCCAAGCTGTTCCAGTCGGTGACCAGCGCCGAGGCG
AAAGTCGACGAGCGCGGTTCAATCACTGTCGATGAGCGGACCAACAACATCATTGCCTACCAGACCCAGGACCGCCTCGA
CGAATTGCGTCGCATCGTTGCACAGCTGGATATTCCGGTGCGTCAGGTGATGATCGAGGCGCGCATCGTTGAAGCCAACG
TCGATTACGACAAAAGCCTCGGCGTGCGCTGGGGCGGTTCGGTGCAGAAGGGTAATTGGAATGGCTCCGGGGTCAACGGC
TCTTCGACCACCGTTGGCACGCCGGGCAGCACCAGCACCAACTCGCCGTTCGTCGACCTAGGGACTTCCTCCAACACCTC
GGGGATCGGCATCGCCTTCATTACCGACAATGTGCTGCTGGACCTTGAGCTGACGGCCATGGAAAAAACCGGCAACGGGG
AAATCGTCTCGCAGCCCAAGGTGGTCACGTCTGACAAGGAAACCGCGAAAATCCTCAAGGGCACCGAAATCCCCTATCAG
GAAGCCAGCTCCAGCGGTGCCACTTCAGTGTCGTTCAAGGAAGCCTCGCTGTCCCTGGAAGTGACGCCGCAGATCACCCC
GGACAACCGCATCATCATGGAGGTCAAGGTCACCAAGGACGAACCGGACTACCTGAACAAAGTGCAGGATGTACCGCCGA
TCAAGAAAAACGAGGTCAACGCCAAGGTATTGGTCAACGACGGCGAGACCATCGTGATTGGTGGGGTTTTCTCAAATACT
CAGAGCAAGGTTGTAGATAAGGTGCCATTTCTCGGTGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGACGTGGTTTC
GGAGAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAACAATCAGGCGATTGCTGTGAGTCATTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(526-682)

Bacterial type II/III secretion system short domain (Secretin_N)

(377-442)

Secretin and TonB N terminus short domain (STN)

(303-349)

AMIN domain (AMIN)

(37-121)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.689

100

0.751
  comQ Acinetobacter baylyi ADP1

41.403

100

0.436
  pilQ Acinetobacter baumannii strain A118

38.911

100

0.424
  pilQ Acinetobacter baumannii D1279779

38.911

100

0.424
  pilQ Legionella pneumophila strain Lp02

37.666

97.974

0.369
  pilQ Legionella pneumophila strain ERS1305867

37.666

97.974

0.369

Multiple sequence alignment