Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   CNN82_RS28130 Genome accession   NZ_CP023466
Coordinates   6131726..6132790 (-) Length   354 a.a.
NCBI ID   WP_019581855.1    Uniprot ID   A0AB33EI66
Organism   Pseudomonas frederiksbergensis strain KNU-15     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6126726..6137790
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CNN82_RS28105 (CNN82_28105) aroK 6127404..6127922 (-) 519 WP_019581860.1 shikimate kinase AroK -
  CNN82_RS28110 (CNN82_28110) pilQ 6127927..6130002 (-) 2076 WP_096481367.1 type IV pilus secretin PilQ Machinery gene
  CNN82_RS28115 (CNN82_28115) - 6130016..6130543 (-) 528 WP_019581858.1 pilus assembly protein PilP -
  CNN82_RS28120 (CNN82_28120) pilO 6130540..6131163 (-) 624 WP_019581857.1 type 4a pilus biogenesis protein PilO -
  CNN82_RS28125 (CNN82_28125) - 6131160..6131726 (-) 567 WP_019581856.1 PilN domain-containing protein -
  CNN82_RS28130 (CNN82_28130) comM 6131726..6132790 (-) 1065 WP_019581855.1 pilus assembly protein PilM Machinery gene
  CNN82_RS28135 (CNN82_28135) - 6133005..6135446 (+) 2442 WP_019581854.1 penicillin-binding protein 1A -
  CNN82_RS28140 (CNN82_28140) - 6135580..6136848 (-) 1269 WP_019581853.1 malic enzyme-like NAD(P)-binding protein -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38096.46 Da        Isoelectric Point: 4.6675

>NTDB_id=247276 CNN82_RS28130 WP_019581855.1 6131726..6132790(-) (comM) [Pseudomonas frederiksbergensis strain KNU-15]
MLGLFNKKTNALLGIDISSTSVKLLELSRQGDRYRVEAYAVEPLPASAVVEKNIAELEGVGQALARVLLKAKTSQRNVAV
AVAGSAVITKTIEMDAGLSDDEMENQLKIEADQYIPYPLDEVAIDFEVQGASVRNPERVNVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLATQLAASQEHLTVAVVDIGATMTTLSVLNNGRIIYTREQLFGGRQLTEEIQRRYGLTV
EQAGLAKKQGGLPDDYVSEVLQPFRDALVQQVSRSLQFFFASGQYSSVDHILLAGGTASVPGLDRLIEQRLGTPTQVANP
FSDMTLSSRVNASALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=247276 CNN82_RS28130 WP_019581855.1 6131726..6132790(-) (comM) [Pseudomonas frederiksbergensis strain KNU-15]
GTGCTAGGACTCTTCAATAAAAAAACCAATGCGCTTCTGGGGATCGACATCAGCTCTACGTCGGTGAAGCTGTTGGAGTT
AAGCCGCCAAGGCGACCGTTATCGGGTCGAGGCCTATGCGGTCGAGCCGCTGCCCGCCAGTGCCGTGGTTGAAAAAAATA
TTGCCGAGCTCGAAGGCGTGGGGCAGGCCCTTGCGCGCGTGCTGCTCAAGGCCAAAACCAGCCAGAGGAACGTCGCCGTG
GCGGTCGCCGGCTCGGCGGTGATCACCAAGACCATCGAGATGGACGCCGGTCTTTCCGACGACGAGATGGAAAACCAGCT
GAAGATCGAGGCCGATCAGTACATCCCGTATCCGCTGGATGAAGTGGCGATCGACTTCGAAGTGCAAGGCGCGTCAGTGC
GCAATCCCGAGCGGGTCAATGTGCTGCTGGCCGCCTGTCGCAAGGAAAACGTCGAGGTTCGTGAAGCGGCCCTGGCCCTG
GCCGGGCTGACCGCGCGAGTAGTCGATGTCGAAGCCTATGCCCTGGAGCGATCGTTCGGCCTGCTGGCAACCCAGCTGGC
CGCTTCTCAGGAGCATCTGACCGTGGCGGTGGTCGATATCGGCGCCACCATGACCACCCTGAGCGTGTTGAACAATGGGC
GGATCATCTACACCCGCGAACAATTGTTCGGCGGCCGACAACTGACGGAAGAAATCCAGCGCCGTTATGGCCTGACGGTC
GAGCAGGCAGGGTTGGCGAAGAAACAGGGCGGTTTGCCGGACGATTACGTCAGTGAGGTCTTGCAGCCGTTTCGGGATGC
GTTGGTGCAACAAGTATCGCGCTCATTGCAGTTTTTCTTTGCCTCCGGCCAATACAGCTCGGTCGATCACATTCTCCTGG
CCGGCGGCACTGCCTCGGTCCCAGGGCTGGATCGGTTGATCGAGCAGCGCCTGGGTACGCCGACCCAGGTCGCTAACCCA
TTCTCCGACATGACCCTGAGCAGCAGGGTCAATGCCAGTGCCCTGGCGAGCGATGCGCCGGCACTGATGATCGCCTGCGG
GCTGGCCCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Acinetobacter nosocomialis M2

56.78

100

0.568
  pilM Acinetobacter baumannii D1279779

56.497

100

0.565
  comM Acinetobacter baylyi ADP1

54.237

100

0.542
  pilM Legionella pneumophila strain ERS1305867

48.588

100

0.486

Multiple sequence alignment