Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CNO08_RS19085 Genome accession   NZ_CP023465
Coordinates   4772375..4774102 (+) Length   575 a.a.
NCBI ID   WP_046657789.1    Uniprot ID   -
Organism   Lysobacter capsici strain KNU-14     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 4761267..4771379 4772375..4774102 flank 996


Gene organization within MGE regions


Location: 4761267..4774102
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CNO08_RS19035 (CNO08_19035) pilA2 4761267..4761677 (+) 411 WP_096415524.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  CNO08_RS19040 (CNO08_19040) - 4761806..4763698 (+) 1893 WP_157754139.1 hypothetical protein -
  CNO08_RS19045 (CNO08_19045) - 4763695..4765707 (+) 2013 WP_157754140.1 hypothetical protein -
  CNO08_RS19050 (CNO08_19050) - 4765926..4766900 (+) 975 WP_157754141.1 hypothetical protein -
  CNO08_RS19055 (CNO08_19055) - 4766887..4767636 (+) 750 WP_096415536.1 class I SAM-dependent methyltransferase -
  CNO08_RS19060 (CNO08_19060) - 4767633..4768694 (+) 1062 WP_096415539.1 NAD(P)-binding domain-containing protein -
  CNO08_RS19065 (CNO08_19065) - 4768674..4769528 (-) 855 WP_157754142.1 GNAT family N-acetyltransferase -
  CNO08_RS19070 (CNO08_19070) - 4769521..4770678 (-) 1158 WP_096415545.1 glycosyltransferase -
  CNO08_RS19075 (CNO08_19075) - 4770675..4771379 (-) 705 WP_051547075.1 class I SAM-dependent methyltransferase -
  CNO08_RS19080 (CNO08_19080) - 4771376..4772239 (-) 864 WP_096415548.1 glycosyltransferase family 2 protein -
  CNO08_RS19085 (CNO08_19085) pilB 4772375..4774102 (+) 1728 WP_046657789.1 type IV-A pilus assembly ATPase PilB Machinery gene

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 62881.40 Da        Isoelectric Point: 6.3668

>NTDB_id=247244 CNO08_RS19085 WP_046657789.1 4772375..4774102(+) (pilB) [Lysobacter capsici strain KNU-14]
MSTVATANLVGITGIARRLVMDGALDEVKAREAMTAASAERKPLASYLAEHRLATPAQLAAANSIEFGVPLFDPSTMDPG
QAPIRAISEELIRKHNALPLFKRGNKLFIGLADPTNTKALDEIKFQTNAAVEAILVDEELIRRTLDRWLETSDALGDAVG
DGEGLDNLEIGKDDDLNTTSDTGIDAKGDDTPVVKFINKVLVDAIRRGASDIHFEPYETDYRVRLRIDGLLKQVAKVPNK
LHPRIAARLKVMAQLDIAEKRVPQDGRIKLNLSKTKQIDFRMSTLPTLFGEKIVLRILDGSAARLGIEKLGYEDYQKELF
VNAVVKPYGMVLVTGPTGSGKTVSLYTALNILNDEQRNISTVEDPVEIRVPGINQVQMNVKRGMTFAAALRSFLRQDPDV
IMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGVAPYNITSSVTLVIAQRLARRLHDCKREVHLPEHA
LLAEGFTHEEIASGFKLYEPVGCPDCTEGYKGRTGIYQVMPMTDEIQAIVLEGGNAMQIAAAAIRSGVPDLRRSALIKVM
NGVTGLAEINRVTKD

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=247244 CNO08_RS19085 WP_046657789.1 4772375..4774102(+) (pilB) [Lysobacter capsici strain KNU-14]
ATGTCCACTGTCGCGACCGCCAACTTGGTGGGAATCACCGGCATTGCCCGGCGTTTGGTCATGGATGGGGCTCTGGACGA
GGTCAAGGCGCGCGAAGCCATGACCGCCGCCAGCGCCGAACGCAAGCCGCTGGCCTCCTACCTGGCCGAACACCGCCTGG
CCACTCCGGCCCAGCTCGCCGCCGCCAACTCGATCGAGTTCGGCGTGCCGCTGTTCGACCCGAGCACCATGGATCCGGGC
CAGGCCCCGATCCGGGCGATCAGCGAAGAGCTGATCCGCAAGCACAACGCCTTGCCGCTGTTCAAGCGCGGCAACAAGCT
GTTCATCGGCCTGGCCGATCCGACCAACACCAAGGCCCTGGACGAGATCAAGTTCCAGACCAATGCCGCGGTCGAGGCGA
TCTTGGTCGATGAGGAACTGATCCGCCGCACCCTGGACCGCTGGCTGGAGACCTCCGACGCCCTCGGCGACGCGGTCGGC
GACGGCGAGGGCCTGGACAACCTGGAGATCGGCAAGGACGACGACCTCAACACCACCAGCGACACCGGTATCGACGCCAA
GGGCGACGACACCCCGGTGGTCAAGTTCATCAACAAGGTGCTGGTCGACGCGATCCGCCGCGGCGCGTCCGACATCCATT
TCGAACCTTACGAAACCGACTACCGGGTGCGCCTGCGCATCGACGGCCTGCTCAAGCAGGTCGCCAAAGTGCCGAACAAG
CTGCATCCGCGCATCGCCGCGCGCCTGAAGGTCATGGCGCAGCTCGACATCGCCGAGAAGCGGGTGCCGCAGGACGGACG
CATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGCATGAGCACCTTGCCGACCCTGTTCGGCGAGAAGATCG
TGCTGCGTATCCTCGACGGCAGCGCGGCACGGCTGGGCATCGAAAAGCTTGGCTACGAGGACTATCAGAAGGAATTGTTC
GTCAACGCGGTGGTCAAGCCCTACGGCATGGTGCTGGTCACCGGCCCGACCGGCTCGGGCAAGACGGTGTCGTTGTACAC
CGCGCTCAACATCCTCAACGACGAACAGCGCAACATCTCCACGGTCGAGGACCCGGTCGAAATCCGCGTGCCGGGCATCA
ATCAGGTGCAGATGAACGTCAAACGCGGCATGACCTTCGCCGCCGCGCTACGCAGCTTCCTGCGTCAGGATCCGGACGTG
ATCATGGTCGGCGAAATCCGCGACCTCGAAACCGCCGAGATCGCGATCAAGGCCGCCCAGACCGGCCACATGGTGCTGTC
GACCCTGCACACCAACGACGCGCCGCAGACCATCGCCCGTCTGATGAACATGGGCGTGGCGCCGTACAACATCACCTCGT
CGGTCACCCTGGTGATCGCACAGCGTCTCGCCCGCCGTCTGCACGACTGCAAGCGCGAGGTGCATCTGCCCGAACACGCG
CTGCTCGCCGAGGGGTTCACCCACGAGGAGATCGCGTCGGGTTTCAAGTTGTACGAGCCGGTCGGATGTCCGGACTGTAC
CGAAGGCTACAAGGGACGTACCGGCATCTATCAGGTCATGCCCATGACCGACGAAATCCAGGCCATCGTGCTCGAAGGCG
GCAATGCGATGCAGATCGCCGCCGCGGCGATCCGATCGGGCGTGCCCGATCTACGCCGCTCCGCGCTGATCAAGGTGATG
AACGGCGTCACCGGCCTGGCCGAAATCAACCGCGTAACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(48-152)

Type II/IV secretion system protein (T2SSE)

(199-466)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.556

98.609

0.548
  pilB Acinetobacter baumannii D1279779

55.36

98.957

0.548
  pilB Legionella pneumophila strain ERS1305867

50.438

99.304

0.501
  pilB Vibrio cholerae strain A1552

49.215

99.652

0.49
  pilF Neisseria gonorrhoeae MS11

47.619

98.609

0.47
  pilB Vibrio parahaemolyticus RIMD 2210633

49.617

90.783

0.45
  pilB Vibrio campbellii strain DS40M4

51.738

85.043

0.44
  pilB/pilB1 Synechocystis sp. PCC 6803

35.831

100

0.383
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.242

100

0.376
  pilF Thermus thermophilus HB27

39.698

92

0.365

Multiple sequence alignment