Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   CMV25_RS00440 Genome accession   NZ_CP023392
Coordinates   94671..95522 (-) Length   283 a.a.
NCBI ID   WP_061774798.1    Uniprot ID   A0A2A5SB33
Organism   Lactococcus raffinolactis strain WiKim0068     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 62356..115666 94671..95522 within 0


Gene organization within MGE regions


Location: 62356..115666
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CMV25_RS00275 (CMV25_00275) - 62873..64837 (-) 1965 WP_096039191.1 KUP/HAK/KT family potassium transporter -
  CMV25_RS00280 (CMV25_00280) - 65194..65751 (-) 558 WP_096039192.1 hypothetical protein -
  CMV25_RS00285 (CMV25_00285) - 65763..66095 (-) 333 WP_096039193.1 holin -
  CMV25_RS00290 (CMV25_00290) - 66108..67832 (-) 1725 WP_096039194.1 BppU family phage baseplate upper protein -
  CMV25_RS00295 (CMV25_00295) - 67813..68964 (-) 1152 WP_096039195.1 hypothetical protein -
  CMV25_RS00300 (CMV25_00300) - 69076..70143 (-) 1068 WP_157738463.1 phage tail spike protein -
  CMV25_RS00305 (CMV25_00305) - 70140..70832 (-) 693 WP_096039197.1 phage tail domain-containing protein -
  CMV25_RS00310 (CMV25_00310) - 70836..73571 (-) 2736 WP_096039198.1 phage tail tape measure protein -
  CMV25_RS00315 (CMV25_00315) - 73693..73890 (-) 198 WP_096039199.1 hypothetical protein -
  CMV25_RS00320 (CMV25_00320) - 73896..74255 (-) 360 WP_096039200.1 hypothetical protein -
  CMV25_RS00325 (CMV25_00325) - 74324..74911 (-) 588 WP_096039201.1 major tail protein -
  CMV25_RS00330 (CMV25_00330) - 74943..76127 (-) 1185 WP_096039202.1 hypothetical protein -
  CMV25_RS00335 (CMV25_00335) - 76128..76481 (-) 354 WP_244165975.1 HK97 gp10 family phage protein -
  CMV25_RS00340 (CMV25_00340) - 76481..76789 (-) 309 WP_096039204.1 phage head closure protein -
  CMV25_RS00345 (CMV25_00345) - 76789..77064 (-) 276 WP_096039205.1 hypothetical protein -
  CMV25_RS00350 (CMV25_00350) - 77057..77245 (-) 189 WP_096039206.1 hypothetical protein -
  CMV25_RS00355 (CMV25_00355) - 77300..78520 (-) 1221 WP_096039207.1 phage major capsid protein -
  CMV25_RS00360 (CMV25_00360) - 78511..79092 (-) 582 WP_157738464.1 HK97 family phage prohead protease -
  CMV25_RS00365 (CMV25_00365) - 79089..80297 (-) 1209 WP_096039208.1 phage portal protein -
  CMV25_RS00370 (CMV25_00370) - 80319..82130 (-) 1812 WP_096039209.1 terminase large subunit domain-containing protein -
  CMV25_RS00375 (CMV25_00375) - 82131..82502 (-) 372 WP_096039210.1 P27 family phage terminase small subunit -
  CMV25_RS00380 (CMV25_00380) - 82660..82980 (-) 321 WP_096039211.1 HNH endonuclease -
  CMV25_RS00385 (CMV25_00385) - 82980..83180 (-) 201 WP_096039212.1 hypothetical protein -
  CMV25_RS00390 (CMV25_00390) - 83603..84430 (-) 828 WP_096039213.1 hypothetical protein -
  CMV25_RS00395 (CMV25_00395) - 84511..85407 (-) 897 WP_096039214.1 hypothetical protein -
  CMV25_RS00400 (CMV25_00400) - 85496..86314 (-) 819 WP_096039215.1 hypothetical protein -
  CMV25_RS11710 - 86402..86524 (-) 123 WP_279627274.1 hypothetical protein -
  CMV25_RS00405 (CMV25_00405) - 86567..87646 (-) 1080 WP_096039216.1 hypothetical protein -
  CMV25_RS00410 (CMV25_00410) - 87657..87842 (-) 186 WP_096039217.1 hypothetical protein -
  CMV25_RS00415 (CMV25_00415) - 89215..90123 (-) 909 WP_096039218.1 tyrosine-type recombinase/integrase -
  CMV25_RS00420 (CMV25_00420) purB 90423..91715 (-) 1293 WP_096039219.1 adenylosuccinate lyase -
  CMV25_RS00425 (CMV25_00425) purK 91746..92804 (-) 1059 WP_096039220.1 5-(carboxyamino)imidazole ribonucleotide synthase -
  CMV25_RS00430 (CMV25_00430) purE 92797..93291 (-) 495 WP_096039221.1 5-(carboxyamino)imidazole ribonucleotide mutase -
  CMV25_RS00435 (CMV25_00435) purD 93284..94537 (-) 1254 WP_061774797.1 phosphoribosylamine--glycine ligase -
  CMV25_RS00440 (CMV25_00440) dprA 94671..95522 (-) 852 WP_061774798.1 DNA-processing protein DprA Machinery gene
  CMV25_RS00445 (CMV25_00445) - 95604..96560 (-) 957 WP_061774799.1 ABC transporter permease -
  CMV25_RS00450 (CMV25_00450) - 96560..97636 (-) 1077 WP_061774800.1 ABC transporter permease -
  CMV25_RS00455 (CMV25_00455) - 97639..99153 (-) 1515 WP_096039222.1 ABC transporter ATP-binding protein -
  CMV25_RS00460 (CMV25_00460) deoD 99302..100012 (-) 711 WP_096039223.1 purine-nucleoside phosphorylase -
  CMV25_RS00465 (CMV25_00465) - 100012..100824 (-) 813 WP_171031981.1 purine-nucleoside phosphorylase -
  CMV25_RS00470 (CMV25_00470) - 100824..102041 (-) 1218 WP_096039225.1 phosphopentomutase -
  CMV25_RS00475 (CMV25_00475) - 102111..103163 (-) 1053 WP_061774805.1 BMP family protein -
  CMV25_RS00480 (CMV25_00480) - 103314..103706 (-) 393 WP_096039226.1 cytidine deaminase -
  CMV25_RS00485 (CMV25_00485) deoC 103690..104355 (-) 666 WP_096039227.1 deoxyribose-phosphate aldolase -
  CMV25_RS00490 (CMV25_00490) - 104352..105641 (-) 1290 WP_096039228.1 pyrimidine-nucleoside phosphorylase -
  CMV25_RS00495 (CMV25_00495) lepB 105853..106413 (-) 561 WP_096039229.1 signal peptidase I -
  CMV25_RS00500 (CMV25_00500) - 106525..106731 (-) 207 WP_096039230.1 DUF2969 family protein -
  CMV25_RS00505 (CMV25_00505) trxA 106905..107222 (-) 318 WP_003136945.1 thioredoxin -
  CMV25_RS00510 (CMV25_00510) - 107330..109843 (-) 2514 WP_096039231.1 ABC transporter permease -
  CMV25_RS00515 (CMV25_00515) - 109848..110549 (-) 702 WP_096039232.1 ABC transporter ATP-binding protein -
  CMV25_RS00520 (CMV25_00520) - 110718..111401 (-) 684 WP_096039233.1 hypothetical protein -
  CMV25_RS00525 (CMV25_00525) - 111615..113174 (-) 1560 WP_096039234.1 ABC-F family ATP-binding cassette domain-containing protein -
  CMV25_RS00530 (CMV25_00530) - 113595..114068 (-) 474 WP_096039235.1 ABC transporter ATP-binding protein -
  CMV25_RS00535 (CMV25_00535) - 114065..115666 (-) 1602 WP_096039236.1 ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 283 a.a.        Molecular weight: 32073.10 Da        Isoelectric Point: 8.4451

>NTDB_id=246577 CMV25_RS00440 WP_061774798.1 94671..95522(-) (dprA) [Lactococcus raffinolactis strain WiKim0068]
MQNFDIYRLKKAGMSNLMVYQFLTLVADSQERNPLVCLPAFIRQVKMKQSAHFWEKFRSFDIKAIREEFQKFPSFSIQDK
VYPEALREIYNPPVLLFYQGDLSLLHQTKLGFVGSRDCSQVGVASVQKIIKELQDRFVIVSGLARGIDTASHIAAMKNGG
KTIAVIGTGLDVNYPRENYQLQAYIAKHQLLLSEYGPDEKPLKYHFPERNRIIAGLSRGVLVTEAKLRSGSLITCERAME
EGRDVFAVPGNIIDGVSDGCHHLIQEGAKLIFHGRDILMEYDM

Nucleotide


Download         Length: 852 bp        

>NTDB_id=246577 CMV25_RS00440 WP_061774798.1 94671..95522(-) (dprA) [Lactococcus raffinolactis strain WiKim0068]
ATGCAAAATTTCGATATCTATCGCTTAAAAAAAGCTGGCATGTCAAACTTAATGGTTTATCAGTTTTTAACCCTAGTTGC
AGACAGTCAAGAAAGAAACCCCTTGGTCTGTTTACCAGCCTTTATTCGACAAGTTAAGATGAAACAGTCAGCTCATTTTT
GGGAAAAATTCCGATCATTTGACATAAAAGCCATACGAGAGGAGTTTCAGAAATTTCCGTCTTTCTCAATTCAGGATAAG
GTCTATCCTGAAGCTTTGCGAGAAATTTATAATCCGCCCGTCTTACTTTTTTATCAGGGAGATTTGAGCTTGCTTCATCA
GACCAAACTGGGTTTTGTTGGCAGTCGAGATTGCAGTCAAGTAGGGGTTGCCAGTGTTCAGAAGATCATCAAAGAATTGC
AAGATCGCTTTGTGATTGTTAGTGGCTTAGCACGGGGCATTGATACAGCTAGTCATATTGCTGCTATGAAAAATGGAGGT
AAGACAATTGCTGTGATTGGAACAGGGTTAGATGTGAATTATCCACGAGAAAATTATCAACTGCAGGCTTATATCGCAAA
ACATCAGCTCCTTTTATCAGAATATGGGCCAGATGAAAAACCTTTGAAGTATCATTTTCCAGAGCGTAATCGAATTATTG
CAGGTCTATCTCGTGGTGTATTAGTGACAGAAGCCAAGCTCCGCTCAGGTAGTTTGATTACCTGTGAGCGGGCTATGGAA
GAGGGGAGAGATGTGTTTGCCGTTCCAGGTAATATCATAGATGGGGTGTCAGATGGCTGCCACCATTTGATTCAGGAAGG
TGCAAAACTGATTTTCCATGGTCGAGATATATTAATGGAGTACGACATGTGA

Domains

Predicted by InterproScan.

DNA recombination-mediator protein A (DNA_processg_A)

(75-271)

DNA processing protein A sterile alpha motif domain (SAM_DprA)

(1-64)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2A5SB33

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Streptococcus mutans UA159

62.898

100

0.629
  dprA Lactococcus lactis subsp. cremoris KW2

60.498

99.293

0.601
  dprA/cilB/dalA Streptococcus mitis NCTC 12261

58.156

99.647

0.58
  dprA/cilB/dalA Streptococcus pneumoniae Rx1

58.156

99.647

0.58
  dprA/cilB/dalA Streptococcus pneumoniae D39

58.156

99.647

0.58
  dprA/cilB/dalA Streptococcus pneumoniae R6

58.156

99.647

0.58
  dprA/cilB/dalA Streptococcus pneumoniae TIGR4

58.156

99.647

0.58
  dprA/cilB/dalA Streptococcus mitis SK321

57.801

99.647

0.576
  dprA Bacillus subtilis subsp. subtilis str. 168

49.321

78.092

0.385
  dprA Legionella pneumophila strain ERS1305867

40.23

92.226

0.371
  dprA Staphylococcus aureus MW2

35.664

100

0.36

Multiple sequence alignment