Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CK207_RS02895 Genome accession   NZ_CP023054
Coordinates   565578..567266 (-) Length   562 a.a.
NCBI ID   WP_029388146.1    Uniprot ID   A0A289G7S6
Organism   Vibrio anguillarum strain VIB43     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 560578..572266
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CK207_RS02865 (CK207_02865) rplS 561307..561660 (+) 354 WP_010319525.1 50S ribosomal protein L19 -
  CK207_RS02870 (CK207_02870) yacG 561730..561927 (-) 198 WP_010319526.1 DNA gyrase inhibitor YacG -
  CK207_RS02875 (CK207_02875) zapD 562017..562757 (-) 741 WP_019281903.1 cell division protein ZapD -
  CK207_RS02880 (CK207_02880) coaE 562790..563398 (-) 609 WP_013857688.1 dephospho-CoA kinase -
  CK207_RS02885 (CK207_02885) pilD 563389..564270 (-) 882 WP_019281902.1 A24 family peptidase Machinery gene
  CK207_RS02890 (CK207_02890) pilC 564309..565535 (-) 1227 WP_069212069.1 type II secretion system F family protein Machinery gene
  CK207_RS02895 (CK207_02895) pilB 565578..567266 (-) 1689 WP_029388146.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CK207_RS02900 (CK207_02900) - 567272..567706 (-) 435 WP_050934217.1 pilin -
  CK207_RS02905 (CK207_02905) nadC 567962..568849 (-) 888 WP_019281900.1 carboxylating nicotinate-nucleotide diphosphorylase -
  CK207_RS02910 (CK207_02910) ampD 568974..569525 (+) 552 WP_214650766.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  CK207_RS02915 (CK207_02915) fldB 569547..570065 (-) 519 WP_013857680.1 flavodoxin FldB -
  CK207_RS02920 (CK207_02920) xerD 570218..571126 (+) 909 WP_013857679.1 site-specific tyrosine recombinase XerD -
  CK207_RS02925 (CK207_02925) - 571140..571913 (+) 774 WP_017045785.1 thioredoxin fold domain-containing protein -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62291.43 Da        Isoelectric Point: 6.4458

>NTDB_id=244600 CK207_RS02895 WP_029388146.1 565578..567266(-) (pilB) [Vibrio anguillarum strain VIB43]
MLTNLPAILRQAQILSPTQEHAVIEHIKASGANVPEALLALDFFHPHDLTEQLSTLFGLPDTDLTRYDYPQLCQQLGLRD
LITRYQALPIAHNGQTLTIAVSDPTHLQVEDDFRFATGLQVELVLADYKALQAAIRRLYGRTIQGSSSSGKEISQDELAN
LVKLSDDEIQSIEDLSQDDAPVSRFINQILLDAVRKGASDIHFEPYEALYRVRLRCDGILVETQRPSSNLSRRLAARLKI
LSKLDIAERRLPQDGRIKLRLNQDTAIDMRVSSLPTLWGEKIVLRLLDSSAANLDIDKLGYNDQQKQLYLAALKKPQGMI
LMTGPTGSGKTVSLYTGLRILNTAEVNISTAEDPVEINLSGINQVQVQPKIGFGFAQALRSFLRQDPDIVMVGEIRDLET
AEIAVKAAQTGHLVLSTLHTNSAAETVIRLSNMGIEAFNLASSLSLIIAQRLARRLCRHCKIAVVPSMQLQQHFQLLATE
TIYEANPHGCNECTGGYSGRVGIYEVMAFNPQLAEAIMQKASIHQIEQIAKANGMQTLQESGIEKLKNGVTSFKELQRVL
YF

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=244600 CK207_RS02895 WP_029388146.1 565578..567266(-) (pilB) [Vibrio anguillarum strain VIB43]
ATGCTAACCAACCTCCCTGCGATTCTGCGTCAGGCTCAGATATTGAGCCCGACGCAAGAACACGCAGTTATTGAACATAT
CAAAGCGTCTGGTGCGAATGTGCCAGAAGCTTTGCTCGCTTTGGATTTTTTCCATCCTCATGATCTGACTGAGCAGTTAA
GTACCCTTTTTGGCCTACCGGACACCGACTTAACCCGCTACGACTACCCACAGCTCTGCCAACAGCTTGGTTTGCGTGAT
CTCATCACCCGTTACCAAGCACTGCCGATTGCCCATAATGGCCAAACCCTGACCATCGCGGTTTCCGACCCGACCCATTT
ACAAGTGGAAGATGATTTTCGCTTCGCGACAGGCTTACAAGTTGAACTGGTTTTAGCCGACTACAAAGCGCTGCAAGCAG
CCATCCGGCGCTTGTATGGCCGAACGATTCAAGGATCCTCTTCTTCAGGGAAAGAGATCAGCCAAGATGAACTGGCGAAC
TTAGTTAAGCTATCTGATGATGAGATTCAATCGATTGAAGACCTCAGCCAAGATGACGCCCCAGTCAGCCGTTTTATTAA
CCAAATCCTGCTTGATGCGGTGCGAAAAGGCGCATCCGATATCCATTTCGAACCGTACGAAGCGCTTTACCGAGTACGAT
TGCGCTGTGATGGTATTTTGGTGGAAACGCAGCGTCCCTCAAGCAACTTAAGTCGCCGGCTTGCCGCGCGGCTTAAAATT
TTATCCAAACTCGATATTGCCGAGCGGCGCTTACCACAAGATGGACGAATTAAACTGCGGTTGAATCAAGACACCGCCAT
CGATATGCGCGTTTCCAGCCTCCCCACTTTATGGGGTGAAAAAATCGTGCTACGTCTACTCGATAGCAGCGCTGCTAACT
TAGATATCGACAAATTGGGTTATAACGACCAGCAAAAACAGCTCTACTTAGCTGCGCTGAAAAAACCACAAGGGATGATT
TTAATGACGGGCCCGACGGGCAGCGGAAAAACCGTCTCGCTCTATACGGGGCTACGCATTTTAAATACTGCTGAAGTGAA
CATTTCCACCGCTGAAGATCCGGTTGAAATTAACTTATCCGGTATCAACCAAGTTCAAGTGCAACCAAAAATTGGCTTTG
GCTTTGCGCAAGCATTACGCTCCTTCTTACGGCAAGATCCCGACATTGTGATGGTTGGCGAAATCCGCGATTTAGAAACT
GCCGAAATCGCCGTGAAAGCGGCACAAACCGGGCATTTAGTTCTTTCTACCTTGCATACTAACTCCGCCGCCGAAACGGT
AATTCGCTTGAGTAATATGGGAATTGAAGCGTTTAACTTAGCCTCTTCACTCAGTTTAATTATTGCCCAGCGTTTGGCTC
GTCGCCTTTGTCGGCACTGCAAAATTGCCGTCGTGCCCTCAATGCAGTTGCAGCAACATTTTCAGCTGCTAGCGACAGAA
ACCATTTATGAAGCGAACCCGCATGGTTGCAACGAATGCACAGGGGGCTATTCAGGTCGAGTCGGCATTTATGAAGTGAT
GGCGTTTAACCCACAGCTGGCCGAAGCGATTATGCAAAAAGCAAGCATTCATCAAATAGAACAGATCGCGAAAGCCAACG
GAATGCAGACACTACAAGAATCAGGCATTGAAAAGCTCAAAAACGGCGTCACCAGCTTCAAAGAGCTGCAACGCGTGCTC
TATTTCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-141)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A289G7S6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

83.096

100

0.831
  pilB Vibrio campbellii strain DS40M4

74.377

100

0.744
  pilB Vibrio parahaemolyticus RIMD 2210633

72.954

100

0.73
  pilB Acinetobacter baumannii D1279779

52.115

92.527

0.482
  pilB Legionella pneumophila strain ERS1305867

51.758

91.103

0.472
  pilB Acinetobacter baylyi ADP1

50.38

93.594

0.472
  pilF Neisseria gonorrhoeae MS11

44.248

100

0.445
  pilF Thermus thermophilus HB27

37.589

100

0.377
  pilB/pilB1 Synechocystis sp. PCC 6803

35.763

100

0.375

Multiple sequence alignment