Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   EBN1_RS12245 Genome accession   NC_006513
Coordinates   2572410..2574125 (-) Length   571 a.a.
NCBI ID   WP_011238275.1    Uniprot ID   Q5P275
Organism   Aromatoleum aromaticum EbN1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2567410..2579125
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EBN1_RS12225 (EB_RS12065) - 2569193..2569819 (-) 627 WP_011238271.1 DUF502 domain-containing protein -
  EBN1_RS12230 (EB_RS12070) - 2569835..2570101 (-) 267 WP_011238272.1 zinc ribbon domain-containing protein -
  EBN1_RS12235 (EB_RS12075) pilD 2570240..2571091 (-) 852 WP_011238273.1 A24 family peptidase Machinery gene
  EBN1_RS12240 (EB_RS12080) pilC 2571158..2572393 (-) 1236 WP_011238274.1 type II secretion system F family protein Machinery gene
  EBN1_RS12245 (EB_RS12085) pilB 2572410..2574125 (-) 1716 WP_011238275.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EBN1_RS12250 (EB_RS12090) ccsA 2574241..2575065 (-) 825 WP_011238276.1 cytochrome c biogenesis protein CcsA -
  EBN1_RS12255 (EB_RS12095) ffh 2575108..2576466 (+) 1359 WP_011238277.1 signal recognition particle protein -
  EBN1_RS12260 (EB_RS12100) - 2576483..2577247 (+) 765 WP_011238278.1 EI24 domain-containing protein -
  EBN1_RS12265 (EB_RS12105) - 2577258..2578007 (+) 750 WP_041646293.1 molybdopterin-binding protein -
  EBN1_RS12270 (EB_RS12110) - 2578004..2578777 (+) 774 WP_011238280.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62214.38 Da        Isoelectric Point: 5.2141

>NTDB_id=24118 EBN1_RS12245 WP_011238275.1 2572410..2574125(-) (pilB) [Aromatoleum aromaticum EbN1]
MAANPQAALSGLARAMIQHGRLSETDAMSCTTGAASAPNGFILELAHRKLMTNQAVAWFAAETFGYPLLDISAIDSTSIA
RDAIDRKLMSKHQVVALGKRQNRLTVAAADPSNMRALDEIRFQTGLQVDLVVVEADRLARLVESLAETAEQTLRELTGEE
FDMDLLQQQEGPGDAPSEDTQEVDDAPVVKFIQKVLIDAINEGASDIHFEPYEKYYRIRVRTDGVLREIAQPPLILKEKI
AARIKVISRLDISEKRVPQDGRMKLVLSKNKSIDFRVSSLPTLHGEKIVMRILDASSAMLGVDALGYEPEQKKILLDAIE
RPYGMILVTGPTGSGKTVSLYTCLNLLNKAGVNISTAEDPAEINLSGINQVNVNDKAGLTFAASLRAFLRQDPDIIMVGE
IRDLETAEISIKAAQTGHLVLSTLHTNDAPTTLERLKNMGVAPFNIASSVILITAQRLARRLCTCKQPVDIPIEALLQAG
FPADELDGSWQPYGPVGCERCKGSGYKGRLGIYQVMPITDEIAHIIMTNGNSMDIAAQAQREGVKDLRQSGLLKVRQGVT
SLEEVLASTNE

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=24118 EBN1_RS12245 WP_011238275.1 2572410..2574125(-) (pilB) [Aromatoleum aromaticum EbN1]
ATGGCAGCGAATCCCCAGGCCGCGCTGAGCGGCCTCGCACGAGCGATGATCCAGCACGGCCGGTTGTCCGAAACCGACGC
CATGTCGTGCACGACAGGCGCTGCCAGCGCGCCGAACGGATTCATCCTCGAGCTTGCCCATCGCAAACTGATGACGAACC
AGGCGGTCGCCTGGTTCGCCGCCGAAACGTTCGGCTACCCGCTGCTCGACATCTCGGCTATCGACAGCACGAGCATTGCG
CGCGACGCGATCGACCGCAAGCTGATGAGCAAGCACCAGGTCGTCGCGCTCGGCAAGCGGCAGAACCGGCTGACGGTCGC
TGCCGCCGATCCGTCGAACATGCGCGCGCTCGACGAGATCCGTTTCCAGACCGGTCTCCAGGTGGACCTCGTCGTCGTCG
AAGCGGACCGGCTCGCGCGTCTGGTCGAGTCCCTGGCCGAAACCGCCGAACAGACGCTGCGGGAGCTCACGGGGGAGGAG
TTCGACATGGATCTCCTGCAGCAGCAGGAAGGTCCCGGTGACGCCCCGAGCGAGGACACGCAGGAAGTCGATGACGCGCC
GGTCGTCAAGTTCATCCAGAAAGTGCTGATCGACGCGATCAACGAAGGCGCTTCGGATATCCACTTCGAGCCGTACGAGA
AGTATTACCGCATCCGCGTGCGCACCGACGGCGTGCTGCGCGAAATCGCCCAGCCGCCGCTGATCCTGAAGGAGAAGATC
GCCGCCCGGATCAAGGTCATCTCGCGCCTCGACATCTCGGAAAAGCGCGTCCCCCAGGACGGGCGGATGAAGCTGGTGCT
GTCGAAGAACAAGTCGATCGACTTTCGTGTCTCGTCGCTGCCGACGCTGCATGGCGAAAAGATCGTGATGCGGATTCTCG
ACGCTTCGTCGGCAATGCTCGGCGTCGATGCGCTGGGCTACGAACCGGAACAGAAGAAGATCCTGCTCGACGCGATCGAA
CGGCCCTACGGAATGATCCTCGTGACCGGACCGACCGGTTCCGGCAAGACCGTGTCGCTCTATACCTGCCTGAACCTGCT
CAACAAAGCCGGCGTGAATATCTCCACGGCCGAAGACCCGGCCGAAATCAACTTGTCCGGGATCAACCAGGTCAACGTCA
ACGACAAGGCCGGGCTGACTTTCGCCGCATCGCTGCGGGCCTTTCTGCGCCAGGATCCGGACATCATCATGGTCGGCGAA
ATCCGCGACCTCGAAACCGCCGAGATTTCGATCAAGGCCGCGCAGACCGGCCACCTGGTGCTGTCGACGCTGCATACCAA
CGACGCCCCGACGACGCTCGAGCGCCTGAAGAACATGGGCGTAGCGCCTTTCAACATCGCCTCGTCGGTGATCCTGATCA
CGGCGCAACGGCTGGCGCGGCGCCTGTGCACGTGCAAGCAGCCGGTCGACATCCCGATCGAAGCGCTGCTGCAGGCGGGG
TTTCCCGCCGACGAGCTCGACGGCAGCTGGCAGCCCTATGGCCCGGTCGGCTGCGAGCGCTGCAAGGGCAGCGGCTACAA
GGGGCGGCTCGGGATCTACCAGGTCATGCCGATCACCGACGAGATCGCGCACATCATCATGACCAACGGCAACTCGATGG
ACATCGCTGCGCAGGCCCAGCGCGAAGGCGTCAAGGATCTGCGCCAGTCCGGACTGCTGAAAGTCAGGCAGGGCGTCACA
TCGCTCGAAGAAGTCCTCGCTTCAACCAACGAATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-150)

Type II/IV secretion system protein (T2SSE)

(194-461)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q5P275

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.398

98.949

0.548
  pilB Acinetobacter baumannii D1279779

54.754

99.475

0.545
  pilB Legionella pneumophila strain ERS1305867

51.93

99.825

0.518
  pilF Neisseria gonorrhoeae MS11

55.238

91.944

0.508
  pilB Vibrio cholerae strain A1552

49.174

95.447

0.469
  pilB Vibrio parahaemolyticus RIMD 2210633

48.092

91.769

0.441
  pilB Vibrio campbellii strain DS40M4

48.343

89.842

0.434
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.636

100

0.387
  pilF Thermus thermophilus HB27

40.762

91.944

0.375

Multiple sequence alignment