Detailed information NTG0024116

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: EBN1_RS12235
• Genome accession: NC_006513
• Coordinates: 2570240..2571091 (-)
• Length: 283 a.a.
• NCBI ID: WP_011238273.1
• Uniprot ID: Q5P277
• Organism: Aromatoleum aromaticum EbN1
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2565240..2576091

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EBN1_RS12200 (EB_RS12040)	-	2565635..2565859 (+)	225	WP_011238266.1	cysteine-rich CWC family protein	-
EBN1_RS12205 (EB_RS12045)	nudB	2565856..2566326 (-)	471	WP_011238267.1	dihydroneopterin triphosphate diphosphatase	-
EBN1_RS12210 (EB_RS12050)	-	2566326..2566769 (-)	444	WP_011238268.1	barstar family protein	-
EBN1_RS12215 (EB_RS12055)	-	2566766..2567131 (-)	366	WP_011238269.1	ribonuclease domain-containing protein	-
EBN1_RS12220 (EB_RS12060)	aspS	2567349..2569148 (-)	1800	WP_011238270.1	aspartate--tRNA ligase	-
EBN1_RS12225 (EB_RS12065)	-	2569193..2569819 (-)	627	WP_011238271.1	DUF502 domain-containing protein	-
EBN1_RS12230 (EB_RS12070)	-	2569835..2570101 (-)	267	WP_011238272.1	zinc ribbon domain-containing protein	-
EBN1_RS12235 (EB_RS12075)	pilD	2570240..2571091 (-)	852	WP_011238273.1	A24 family peptidase	Machinery gene
EBN1_RS12240 (EB_RS12080)	pilC	2571158..2572393 (-)	1236	WP_011238274.1	type II secretion system F family protein	Machinery gene
EBN1_RS12245 (EB_RS12085)	pilB	2572410..2574125 (-)	1716	WP_011238275.1	type IV-A pilus assembly ATPase PilB	Machinery gene
EBN1_RS12250 (EB_RS12090)	ccsA	2574241..2575065 (-)	825	WP_011238276.1	cytochrome c biogenesis protein CcsA	-

Sequence

Protein

• Length: 283 a.a.
• Molecular weight: 31098.70 Da
• Isoelectric Point: 6.6862

>NTDB_id=24116 EBN1_RS12235 WP_011238273.1 2570240..2571091(-) (pilD) [Aromatoleum aromaticum EbN1]
MPDLFHDLLLFTALATFVGLFVGSFLNVVIHRLPRMMEREWHAQAAELRGESLPEQETYNLAVPRSRCPHCGHMITAFEN
IPVISYLVLRGRCRHCSAPIGRRYPTVEMLTAVLSGYAAWHFGFGLAAAGAMLFLWTMVALAFIDLDTQLLPDSLTLPLL
WLGLLFNLDGTYSELPQAVVGAIAGYLALWAVYWLFKLATGKEGMGYGDFKLLAAIGAWLGWQMLPLTILFSSLVGAVTG
LGLIVFARHGRDVPIPFGPYLAAAGVLALFWGEPLTQLYLGSL

Nucleotide

• Length: 852 bp

>NTDB_id=24116 EBN1_RS12235 WP_011238273.1 2570240..2571091(-) (pilD) [Aromatoleum aromaticum EbN1]
ATGCCCGACCTCTTTCACGACCTTCTCCTGTTCACCGCGCTCGCGACGTTCGTCGGCCTTTTCGTCGGCAGCTTCCTGAA
CGTCGTCATCCACCGGTTGCCGCGCATGATGGAGCGCGAATGGCACGCCCAGGCTGCCGAGCTGCGCGGCGAATCGCTCC
CCGAGCAGGAAACCTACAATCTGGCGGTGCCGCGCTCGCGCTGCCCGCACTGCGGCCACATGATCACCGCGTTCGAGAAC
ATCCCGGTCATCAGCTATCTTGTCCTGCGCGGGCGATGCCGTCACTGCAGCGCGCCCATTGGCCGCCGCTACCCGACCGT
CGAAATGCTCACCGCTGTGCTCTCGGGCTACGCTGCGTGGCACTTCGGCTTCGGCCTCGCGGCCGCCGGCGCAATGCTTT
TCCTGTGGACAATGGTCGCGCTCGCGTTCATCGATCTCGACACCCAGCTGCTGCCCGACAGCCTTACCCTCCCGCTGCTG
TGGCTCGGCCTGCTTTTCAATCTCGATGGCACGTACAGCGAGCTCCCGCAGGCCGTCGTCGGCGCGATCGCCGGTTACCT
CGCGCTGTGGGCAGTGTACTGGCTGTTCAAGCTCGCCACCGGCAAGGAAGGCATGGGCTACGGCGATTTCAAGCTGCTCG
CCGCGATCGGGGCGTGGCTCGGATGGCAGATGCTGCCCTTGACGATCCTGTTCTCGTCGCTCGTCGGCGCGGTCACCGGC
CTCGGCCTGATCGTTTTCGCCCGGCACGGCCGGGACGTGCCGATTCCGTTCGGCCCCTATCTCGCCGCTGCCGGCGTGCT
GGCGCTGTTCTGGGGCGAGCCGCTCACGCAGTTGTACCTCGGCAGCCTGTGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (132-241)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (18-122)

3D structure

• AlphaFold DB: Q5P277

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio campbellii strain DS40M4	54.513	97.88	0.534
pilD	Vibrio cholerae strain A1552	54.71	97.527	0.534
pilD	Acinetobacter nosocomialis M2	48.54	96.82	0.47
pilD	Acinetobacter baumannii D1279779	48.54	96.82	0.47
pilD	Neisseria gonorrhoeae MS11	50	89.753	0.449