Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   EBN1_RS09525 Genome accession   NC_006513
Coordinates   2015440..2016873 (-) Length   477 a.a.
NCBI ID   WP_011237736.1    Uniprot ID   -
Organism   Aromatoleum aromaticum EbN1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2010440..2021873
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EBN1_RS09500 (EB_RS09375) - 2011402..2011974 (+) 573 WP_041646125.1 5-formyltetrahydrofolate cyclo-ligase -
  EBN1_RS09505 (EB_RS09380) ilvA 2011984..2013504 (-) 1521 WP_011237732.1 threonine ammonia-lyase, biosynthetic -
  EBN1_RS09510 (EB_RS09385) hprK 2013576..2014517 (-) 942 WP_011237733.1 HPr(Ser) kinase/phosphatase -
  EBN1_RS09515 (EB_RS09390) ptsN 2014486..2014971 (-) 486 WP_011237734.1 PTS IIA-like nitrogen regulatory protein PtsN -
  EBN1_RS09520 (EB_RS09395) raiA 2015102..2015425 (-) 324 WP_011237735.1 ribosome-associated translation inhibitor RaiA -
  EBN1_RS09525 (EB_RS09400) rpoN/rpoN1 2015440..2016873 (-) 1434 WP_011237736.1 RNA polymerase factor sigma-54 Machinery gene
  EBN1_RS09530 (EB_RS09405) lptB 2016894..2017616 (-) 723 WP_011237737.1 LPS export ABC transporter ATP-binding protein -
  EBN1_RS09535 (EB_RS09410) recX 2017603..2018052 (-) 450 WP_041646128.1 recombination regulator RecX -
  EBN1_RS09540 (EB_RS09415) recA 2018063..2019103 (-) 1041 WP_011237739.1 recombinase RecA Machinery gene
  EBN1_RS09545 (EB_RS09420) - 2019166..2019666 (-) 501 WP_011237740.1 nicotinamide-nucleotide amidohydrolase family protein -
  EBN1_RS09550 (EB_RS09425) - 2019668..2020147 (-) 480 WP_041647110.1 phosphatidylglycerophosphatase A -
  EBN1_RS09555 (EB_RS09430) thiL 2020205..2021164 (-) 960 WP_011237742.1 thiamine-phosphate kinase -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53196.15 Da        Isoelectric Point: 4.9387

>NTDB_id=24111 EBN1_RS09525 WP_011237736.1 2015440..2016873(-) (rpoN/rpoN1) [Aromatoleum aromaticum EbN1]
MKPTLQLKLSQHLTLTPQLQQSIKLLQLSTLELNQELERFLLENPMLEREDGDGSDFVPPPATAAATGGENENPPAASEQ
HPDAGPANLDEAGEWSSAGSGSSNRDDEDDSDYQEFQAAETSLRDHLDQQVSLSPLSDRDRALVRFIIEALDDDGYLNQS
LEELLPLLPPELEYDLDDLSIALHHVQNLDPAGVAARTPQECLSLQLRAMRPTPARELALKIVDHHLELLAERNFVRLKR
LLGCDDDALRCAHLLICSLDPHPGSQHSVQETRYIMPDVVVRKQRGRWVVTLNPEAMPKLRINSLYASILQQNRGSGGAL
TSQLQEARWLIKNVQQRFDTILRVSQAIVDQQRQFFDYGEVAMRPLTLREIADQLELHESTVSRVTTQKFMATPRGVFEL
KYFFGSHVATDTGGAASSTAIRALIRQLVEAEDGKKPLSDAKIAELLGQQGIVVARRTIAKYRESLNIPPVSLRKTI

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=24111 EBN1_RS09525 WP_011237736.1 2015440..2016873(-) (rpoN/rpoN1) [Aromatoleum aromaticum EbN1]
ATGAAGCCGACCCTCCAGCTCAAACTCTCCCAGCACCTCACGCTGACACCGCAACTGCAGCAGTCGATCAAGCTGTTGCA
GTTATCGACGCTCGAACTGAACCAGGAGCTCGAACGCTTCCTGCTCGAGAACCCGATGCTCGAACGCGAGGACGGCGACG
GCAGCGATTTTGTCCCCCCGCCCGCCACGGCCGCAGCTACAGGCGGCGAAAACGAAAATCCTCCCGCGGCGAGCGAACAG
CACCCCGACGCGGGACCGGCGAACCTCGACGAAGCCGGTGAGTGGTCGAGCGCGGGAAGCGGCTCGTCGAACCGCGACGA
CGAGGACGACAGCGACTACCAGGAGTTCCAGGCGGCGGAAACCAGCCTGCGCGATCACCTCGACCAGCAGGTCAGCCTGT
CGCCGCTGTCCGATCGCGACCGCGCGCTGGTGCGCTTCATCATCGAGGCGCTCGACGACGACGGCTATCTGAACCAGTCG
CTCGAAGAGCTGCTGCCGCTGTTGCCGCCGGAACTCGAATACGACCTCGACGACCTGTCGATCGCACTACACCATGTGCA
GAACCTCGACCCCGCAGGCGTCGCGGCGCGCACGCCGCAGGAATGCCTCAGCCTGCAGCTGCGCGCGATGCGCCCGACTC
CAGCGCGCGAGCTCGCGCTGAAGATCGTCGACCATCACCTCGAGCTTCTTGCCGAACGCAACTTCGTCCGCCTCAAGCGC
CTGCTCGGTTGCGACGACGATGCGCTGCGCTGCGCGCACCTGCTGATCTGCAGCCTCGACCCGCACCCGGGCTCCCAGCA
TTCAGTGCAGGAAACGCGCTACATCATGCCCGACGTCGTCGTGCGCAAGCAGCGCGGGCGCTGGGTCGTCACGCTGAATC
CCGAAGCGATGCCGAAACTGCGCATCAACTCGCTGTACGCCAGCATCCTGCAGCAGAATCGCGGCTCCGGCGGCGCCTTG
ACGAGTCAGTTGCAGGAAGCGCGCTGGCTGATCAAGAACGTGCAGCAGCGTTTCGACACGATCCTGCGCGTCTCCCAGGC
GATCGTTGACCAGCAGCGGCAGTTCTTCGATTATGGCGAGGTGGCGATGCGCCCGCTGACGCTGAGGGAAATCGCGGACC
AACTGGAACTGCACGAATCCACCGTTTCGCGGGTCACGACACAGAAATTCATGGCCACCCCGCGAGGCGTGTTCGAACTG
AAGTATTTTTTCGGCAGTCACGTCGCCACCGATACGGGTGGAGCGGCATCCTCAACGGCGATTCGCGCGCTGATCCGCCA
GTTGGTCGAAGCCGAAGACGGAAAAAAACCTCTGTCCGACGCAAAGATTGCCGAATTGTTGGGCCAGCAGGGTATCGTGG
TGGCGCGGCGCACCATTGCAAAATACCGCGAATCACTCAATATCCCGCCGGTCAGCCTGCGCAAGACGATTTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(113-306)

Sigma-54, DNA binding domain (Sigma54_DBD)

(320-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

56.375

100

0.593

Multiple sequence alignment