Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   OTERR_RS02730 Genome accession   NZ_CP022579
Coordinates   591234..592958 (-) Length   574 a.a.
NCBI ID   WP_149424784.1    Uniprot ID   -
Organism   Oryzomicrobium terrae strain TPP412     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 586234..597958
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OTERR_RS02710 (OTERR_05390) - 587976..588635 (-) 660 WP_149424781.1 DUF502 domain-containing protein -
  OTERR_RS02715 (OTERR_05400) - 588705..588965 (-) 261 WP_054620053.1 zinc ribbon domain-containing protein -
  OTERR_RS02720 (OTERR_05410) pilD 589118..589981 (-) 864 WP_149424782.1 A24 family peptidase Machinery gene
  OTERR_RS02725 (OTERR_05420) pilC 589987..591213 (-) 1227 WP_149424783.1 type II secretion system F family protein Machinery gene
  OTERR_RS02730 (OTERR_05430) pilB 591234..592958 (-) 1725 WP_149424784.1 type IV-A pilus assembly ATPase PilB Machinery gene
  OTERR_RS02735 (OTERR_05440) - 593159..594424 (-) 1266 WP_149424785.1 HlyC/CorC family transporter -
  OTERR_RS02740 (OTERR_05450) ccsA 594449..595300 (-) 852 WP_246154290.1 cytochrome c biogenesis protein CcsA -
  OTERR_RS02745 (OTERR_05460) ffh 595448..596809 (+) 1362 WP_054620047.1 signal recognition particle protein -
  OTERR_RS02750 (OTERR_05470) - 596897..597703 (-) 807 WP_149424787.1 lytic transglycosylase domain-containing protein -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 62340.16 Da        Isoelectric Point: 4.9338

>NTDB_id=240944 OTERR_RS02730 WP_149424784.1 591234..592958(-) (pilB) [Oryzomicrobium terrae strain TPP412]
MAANLQVTFSGLARALVQAGKLQEAEAETLAGQAATSKTSFIEQVVASNKLAPLEVAQFAADTFGYPLLDLRAYDTAHIP
RDAIDRKLISAHQVIPLNKRGNRITIAIADPTNLRALDEIRFQTGLTVDPVVVEEAAIKPLVTEFSESASETLKNLVGDD
INLEFSDEEATAANEEAAATDVDDAPVVKFIQKILLDAINDGASDIHFEPYEKYYRIRFRVDGILREVSTPPLAIKEKIA
SRIKVISRLNIAEKRIPQDGRMKLVLSKSRAIDFRVSTLPTIHGEKIVLRILDPTSATLGIEALGYEPDQKAQLLDAVQR
PYGMVLVTGPTGSGKTVSLYTCLNILNEPGVNISTAEDPAEINLAGINQVNVDDKAGLTFAAALKSFLRQDPDVIMVGEV
RDLETAEIAIKAAQTGHMVLSTLHTNDAPQTLERLRNMGVPLFNIASSVILITAQRLARRLCNCKKPLDVPEQTLRDAGY
SDDEVSAYLRGEWTLYGPGGCDRCKGSGYKGRVGIYQVMPVSEEIQRIIMADGNSLDIAAQAQKDGVRDLRQSGLLKAKQ
GVTSLDEVLSTTNA

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=240944 OTERR_RS02730 WP_149424784.1 591234..592958(-) (pilB) [Oryzomicrobium terrae strain TPP412]
ATGGCCGCCAATCTGCAGGTCACCTTCAGCGGGCTAGCCCGCGCCCTCGTCCAGGCCGGCAAGTTGCAGGAAGCCGAGGC
GGAAACGTTGGCCGGCCAGGCGGCCACGAGCAAGACCTCGTTCATCGAACAAGTGGTTGCGAGCAATAAACTCGCCCCCC
TGGAAGTCGCCCAGTTCGCGGCCGACACCTTCGGCTATCCCCTGCTCGACCTGCGGGCCTACGATACGGCCCACATTCCC
CGGGATGCCATCGACCGCAAGTTGATCTCGGCTCACCAGGTCATACCGCTCAACAAGCGGGGCAACCGCATCACCATTGC
CATCGCCGATCCGACCAACCTGCGCGCCCTGGATGAAATCCGCTTCCAGACCGGGCTGACGGTGGACCCGGTGGTAGTGG
AAGAGGCGGCAATCAAGCCGCTGGTCACCGAATTCTCCGAATCCGCATCGGAAACCCTGAAAAACCTGGTTGGTGACGAC
ATCAACCTGGAGTTTTCCGACGAGGAGGCAACGGCAGCAAACGAAGAAGCTGCCGCCACCGATGTGGACGACGCTCCAGT
CGTCAAATTCATCCAAAAAATCCTCCTCGACGCGATCAACGACGGGGCCTCGGACATCCATTTCGAGCCCTATGAAAAGT
ATTACCGCATCCGTTTCCGGGTAGACGGCATCCTGCGCGAGGTATCCACGCCACCCCTGGCTATCAAGGAAAAAATCGCT
TCCCGGATCAAGGTCATCTCCCGGCTCAACATCGCCGAAAAGCGTATCCCCCAGGATGGCCGGATGAAACTGGTGCTGTC
GAAGAGCCGCGCCATCGACTTCCGGGTCAGCACCCTGCCGACCATTCACGGCGAAAAGATCGTTCTGCGTATTCTCGACC
CGACCTCTGCCACCCTGGGCATCGAGGCCCTCGGCTACGAGCCTGACCAGAAGGCGCAACTGCTCGATGCCGTGCAGCGT
CCCTACGGCATGGTGCTGGTGACCGGCCCCACCGGCTCGGGGAAAACGGTATCGCTATACACCTGCCTCAACATCCTCAA
CGAGCCCGGCGTGAATATTTCCACCGCGGAGGACCCGGCGGAAATCAACCTGGCGGGGATCAACCAGGTCAACGTGGACG
ACAAGGCCGGCCTGACGTTTGCCGCGGCGCTGAAATCCTTCCTCCGCCAGGATCCGGACGTGATCATGGTGGGCGAAGTG
CGCGACCTGGAAACCGCCGAGATCGCTATCAAGGCCGCCCAGACCGGCCACATGGTGCTCTCGACCCTGCACACTAACGA
CGCCCCCCAGACGCTGGAACGCCTGCGCAACATGGGCGTCCCCCTGTTCAATATCGCCTCCTCGGTGATCCTCATCACCG
CCCAGCGACTGGCGCGCCGCCTGTGCAACTGCAAGAAGCCGCTGGACGTGCCCGAGCAAACCCTGCGCGATGCTGGCTAC
AGCGACGACGAGGTTAGCGCCTACCTGCGCGGGGAATGGACGCTCTACGGCCCGGGCGGTTGCGACCGCTGCAAGGGTAG
CGGCTACAAGGGACGGGTCGGCATCTACCAGGTGATGCCGGTATCCGAGGAAATTCAACGCATTATCATGGCCGACGGCA
ACTCCCTGGACATCGCCGCCCAGGCTCAGAAGGACGGGGTCCGCGACCTACGCCAGTCCGGCCTGCTCAAGGCCAAGCAG
GGCGTCACGTCCCTGGATGAAGTCCTGTCCACTACCAACGCCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(53-150)

Type II/IV secretion system protein (T2SSE)

(193-460)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.556

98.78

0.549
  pilB Acinetobacter baylyi ADP1

53.887

98.606

0.531
  pilF Neisseria gonorrhoeae MS11

53.004

98.606

0.523
  pilB Legionella pneumophila strain ERS1305867

52.389

98.432

0.516
  pilB Vibrio cholerae strain A1552

48.155

99.129

0.477
  pilB Vibrio parahaemolyticus RIMD 2210633

47.295

99.826

0.472
  pilB Vibrio campbellii strain DS40M4

46.69

100

0.467
  pilF Thermus thermophilus HB27

41.002

93.902

0.385
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.525

92.857

0.376

Multiple sequence alignment