Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CHQ57_RS20295 Genome accession   NZ_CP022550
Coordinates   4349205..4350911 (+) Length   568 a.a.
NCBI ID   WP_058394588.1    Uniprot ID   -
Organism   Aeromonas salmonicida strain A527     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4344205..4355911
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CHQ57_RS20270 (CHQ57_20160) pdhR 4345339..4346103 (-) 765 WP_005314150.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  CHQ57_RS20275 (CHQ57_20165) ampD 4346379..4346951 (-) 573 WP_099994486.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  CHQ57_RS20280 (CHQ57_20170) - 4347101..4347571 (+) 471 WP_192873266.1 retropepsin-like aspartic protease family protein -
  CHQ57_RS20285 (CHQ57_20175) nadC 4347576..4348433 (+) 858 WP_099994488.1 carboxylating nicotinate-nucleotide diphosphorylase -
  CHQ57_RS20290 (CHQ57_20180) - 4348768..4349205 (+) 438 WP_099994863.1 pilin -
  CHQ57_RS20295 (CHQ57_20185) pilB 4349205..4350911 (+) 1707 WP_058394588.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  CHQ57_RS20300 (CHQ57_20190) pilC 4351040..4352281 (+) 1242 WP_059113460.1 type II secretion system F family protein Machinery gene
  CHQ57_RS20305 (CHQ57_20195) pilD 4352345..4353220 (+) 876 WP_099994489.1 prepilin peptidase Machinery gene
  CHQ57_RS20310 (CHQ57_20200) coaE 4353239..4353853 (+) 615 WP_005314164.1 dephospho-CoA kinase -
  CHQ57_RS20315 (CHQ57_20205) zapD 4353891..4354613 (+) 723 WP_162626251.1 cell division protein ZapD -
  CHQ57_RS20320 (CHQ57_20210) yacG 4354623..4354817 (+) 195 WP_005314167.1 DNA gyrase inhibitor YacG -
  CHQ57_RS20325 (CHQ57_20215) mutT 4354895..4355302 (-) 408 WP_099994491.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62546.97 Da        Isoelectric Point: 5.4384

>NTDB_id=240635 CHQ57_RS20295 WP_058394588.1 4349205..4350911(+) (pilB) [Aeromonas salmonicida strain A527]
MTSSPNSGLALSLAASSLLSESDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAIGKLLESEQDALGMEEIDESEI
SELEVSDENSRLDESVNTADDDAPIVKYINKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEIATPPVNLANRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
QQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRVSGLEKARLGVTSLA
EINRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=240635 CHQ57_RS20295 WP_058394588.1 4349205..4350911(+) (pilB) [Aeromonas salmonicida strain A527]
ATGACCTCTAGCCCCAATAGCGGCCTGGCGTTAAGCCTGGCCGCCTCATCCCTGCTCAGTGAGTCCGACTCACAGCGTTA
CCTGAGCCAAGCCAAGGCCCAGCGCAAACCATTCGTGACATTTCTGATTGAAAACGAGATCCTCGACAGCAAGGCGCTGG
CGGATTTCTGCGAACTGGAGTATGGGGTACCTTTGCTGGATCTTGCCGCTTTCGACCTGGCCGAGATCCCGCAGAAGTAC
CTCAATCAGAAGCTGATTGAGAAACACCATGTGCTGCCCATCTACACCCAGGGACACACCCTCTATATCGCCATGTCGGA
CCCGACCAATGTGTCGGCACTGGAGGATTTTGGCTTCAGCTTCGGGCTCCATACCGAAGCCCTGCTGGTGGAAGAGAACA
AGCTCACCGCCGCCATCGGCAAGCTGCTGGAAAGCGAGCAGGATGCCCTCGGCATGGAGGAGATCGACGAGTCGGAGATC
TCCGAACTGGAAGTCTCGGACGAAAACTCCCGCCTCGATGAGAGCGTCAATACCGCAGATGACGATGCCCCCATCGTCAA
ATACATCAACAAAATCATGATGGATGCCATCAAGCGCGGCGCCTCGGATCTGCACTTCGAACCCTATGAAACCAAGTACC
GTATCCGCTTTCGGATCGATGGCATACTGCACGAAATAGCGACGCCGCCAGTCAATCTGGCCAACCGTTTCTCCGCCCGC
CTCAAGGTGATGGCGCGACTCGATATCGCCGAGCGACGACTGCCCCAGGACGGTCGCATCAAGCTCAAGCTGTCGCGCAA
CAAATCCATGGATATGCGGGTCAACACACTGCCCACCATGTGGGGCGAGAAGATCGTTATCCGTCTGCTGGACTCTTCCG
CAGCGCGACTCAATATCGAACAGCTCGGCTTCGACGACCGGCAGAAGGCACAGTATCTGCGTGCCCTCTCCAAGCCCCAG
GGCATGATATTGGTGACAGGCCCGACCGGTTCTGGCAAGACTGTCTCTCTCTATACCGGCCTCAATATTCTCAACACCAC
AGAGGTCAACATCTCTACCGCCGAAGACCCGGTGGAGATCAACCTGCCCGGCGTCAACCAGGTACAAGTCAATCCCAAGG
CGGGGCTGACCTTCGCCAGCGCCCTGCGCTCCTTTCTTCGCCAGGATCCCGATGTGGTGATGGTGGGGGAAATCCGGGAT
CTGGAAACCGCCGAGATTGCCATAAAAGCCGCCCAGACCGGCCACCTGGTGCTCTCCACCCTGCACACCAACTCGGCTGC
CGAAACCCTGACCCGGATGATGAACATGGGGGTTCCGGCCTTCAATATCGCCTCTTCGGTAACCCTCATCATGGCCCAGC
GCCTCGCTCGCAAGCTCTGCGATCACTGCAAGGCGCCGGAAGTGGTGCCCGAGGCTGAACTGCTGGAGCTGGGCTTCACC
CAGCAGCAGTTGGCCGCCGGCTTGCGCCTGTTCAAACCGGTAGGCTGCAAAGAGTGCTCGGGGGGCTACAAGGGTCGGGT
CGGTATCTACGAAATCATGCTAATGTCTGAAAACATTGCAAAATTGATCATGCAAGGAGCCAACTCCCTGCAAATTGCAG
CCATTGCTCAAAAAGAGGGGATGCGAACCCTGCGTGTCTCGGGCCTGGAGAAGGCCCGCCTGGGGGTGACCAGCCTGGCA
GAAATCAACCGGGTGACCACCAACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.614

100

0.558
  pilB Acinetobacter baumannii D1279779

55.556

99.824

0.555
  pilB Legionella pneumophila strain ERS1305867

54.594

99.648

0.544
  pilB Vibrio cholerae strain A1552

54.513

99.472

0.542
  pilB Vibrio campbellii strain DS40M4

52.12

99.648

0.519
  pilB Vibrio parahaemolyticus RIMD 2210633

51.596

99.296

0.512
  pilF Neisseria gonorrhoeae MS11

46.454

99.296

0.461
  pilB/pilB1 Synechocystis sp. PCC 6803

38.879

97.359

0.379
  pilF Thermus thermophilus HB27

40.341

92.958

0.375

Multiple sequence alignment