Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   CHH28_RS01675 Genome accession   NZ_CP022530
Coordinates   366579..368681 (-) Length   700 a.a.
NCBI ID   WP_233243705.1    Uniprot ID   -
Organism   Bacterioplanes sanyensis strain NV9     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 361579..373681
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CHH28_RS01660 (CHH28_01660) - 363481..364962 (-) 1482 WP_094058682.1 AAA family ATPase -
  CHH28_RS01665 (CHH28_01665) aroB 364983..366062 (-) 1080 WP_094058683.1 3-dehydroquinate synthase -
  CHH28_RS01670 (CHH28_01670) aroK 366059..366577 (-) 519 WP_094058684.1 shikimate kinase AroK -
  CHH28_RS01675 (CHH28_01675) pilQ 366579..368681 (-) 2103 WP_233243705.1 type IV pilus secretin PilQ Machinery gene
  CHH28_RS01680 (CHH28_01680) - 368683..369216 (-) 534 WP_094058685.1 pilus assembly protein PilP -
  CHH28_RS01685 (CHH28_01685) - 369219..369854 (-) 636 WP_233243706.1 type 4a pilus biogenesis protein PilO -
  CHH28_RS01690 (CHH28_01690) - 369854..370405 (-) 552 WP_094058686.1 PilN domain-containing protein -
  CHH28_RS01695 (CHH28_01695) pilM 370405..371469 (-) 1065 WP_094061945.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 700 a.a.        Molecular weight: 76062.55 Da        Isoelectric Point: 4.5444

>NTDB_id=240348 CHH28_RS01675 WP_233243705.1 366579..368681(-) (pilQ) [Bacterioplanes sanyensis strain NV9]
MKTHMLAKFSNKWALTLIGVLFSSLALSVELEELKFTSLPGDVTEIELTFDGTPPEIKGYSIEKPARISIDMAGASSGLD
SKKFDIGSGNARSAIVLGDKNRVRMVINLTRLVDYKTSVEGNSLFVRVGKTAQQTVFRNKSTPTTVASQLSVSELKVVGV
DFRRGELGDGQVQIKLSDDRASADIRREGSKIIADLEGVRLPRELSRRLDVLDFATPVKYVDVRAVDGGSRVVIEPKSDD
FDFLAYQTDRLLSINISDLPEEVAEERKKTFPFTGEKLSLNFQNIEVRAVLQLIADFTGLNLVASDTVQGSITLRLQDVP
WDQALDLVLKTKGLGKRQMGSVLLIAPAEEIAAREKVELEAVRQVEELAPLVTEYMQLNYAKAKDLAELLTSESGLLSDR
GTAVVDERTNTLLMKDTPSNLAKIREAFSLLDVPVRQVLIEARIVVANTDVGEEMGVKWGGAGFKDNGSNWTTIGGSRQT
LTESNQILFDRATQATAGSSTIDLNAADVVNFGVTNTAASSLALGYQTADYLLDLELAAIETDGRGEVVSQPRVMTADGK
TAMIESGTEIPYQEAASSGATSVAFKSAVLKLEVTPQITPDDRIIMDLVVTKDSVGENTVAGPAIDTNAVETQVLVDNGE
TIVLGGIFESEERTTINKTPFFGDLPLIGALFRYKQVEDKKTELLVFITPKLVKDTLDAR

Nucleotide


Download         Length: 2103 bp        

>NTDB_id=240348 CHH28_RS01675 WP_233243705.1 366579..368681(-) (pilQ) [Bacterioplanes sanyensis strain NV9]
ATGAAGACTCACATGCTGGCAAAATTTTCGAATAAATGGGCGCTTACGCTAATAGGGGTTTTGTTTAGTTCGCTAGCCCT
TTCTGTTGAGCTGGAGGAACTGAAGTTTACTTCTCTCCCTGGAGATGTTACTGAAATTGAGCTGACGTTTGATGGCACGC
CTCCCGAGATTAAAGGGTATTCAATAGAAAAGCCTGCGAGGATTTCTATTGATATGGCTGGAGCTAGTAGCGGGCTTGAC
TCTAAAAAGTTTGATATCGGATCTGGCAATGCACGTTCTGCTATTGTTCTTGGTGATAAGAATCGAGTGAGAATGGTCAT
TAATCTGACACGTCTGGTGGACTACAAAACGTCCGTAGAGGGCAATAGCTTGTTTGTGCGAGTAGGTAAAACGGCTCAGC
AGACTGTGTTTAGAAATAAATCTACACCTACAACTGTCGCCTCTCAATTATCTGTCTCTGAGTTGAAAGTTGTCGGAGTT
GACTTTCGCAGAGGTGAACTGGGAGATGGTCAAGTGCAGATCAAGCTTAGTGATGATCGCGCGTCTGCTGACATTCGCAG
AGAAGGCTCTAAAATAATCGCCGATCTTGAGGGTGTGCGCCTTCCTCGAGAGCTCAGTCGCCGCTTAGATGTATTGGATT
TTGCAACGCCAGTTAAATACGTAGATGTTCGAGCTGTTGATGGTGGTTCGAGGGTCGTTATTGAACCTAAATCGGACGAC
TTCGACTTCTTGGCTTATCAAACAGACCGATTGTTATCGATAAATATTTCTGATTTGCCAGAAGAGGTAGCTGAAGAGCG
GAAAAAAACTTTCCCTTTTACTGGTGAAAAGCTCTCTTTGAATTTCCAAAACATCGAAGTTCGTGCAGTACTTCAGCTGA
TCGCTGACTTTACAGGGTTGAATTTGGTCGCCTCTGATACGGTGCAAGGCAGTATCACCTTAAGGCTACAGGACGTTCCA
TGGGATCAAGCGCTTGACCTAGTACTGAAAACGAAAGGGCTTGGTAAACGTCAAATGGGATCTGTGCTTTTAATTGCCCC
GGCAGAGGAGATAGCTGCTCGTGAGAAGGTTGAGCTTGAAGCAGTGCGTCAAGTCGAAGAGCTTGCTCCACTGGTAACAG
AGTACATGCAACTGAACTATGCTAAGGCCAAAGACCTAGCTGAGCTGCTTACATCTGAAAGCGGATTGTTGTCGGATAGA
GGAACAGCCGTCGTAGATGAGCGCACTAATACCTTGTTGATGAAAGATACTCCATCGAACCTCGCAAAAATTCGAGAGGC
TTTTTCCCTGTTAGATGTTCCAGTTCGTCAGGTGTTAATTGAAGCCAGAATTGTTGTGGCTAACACTGATGTTGGTGAGG
AAATGGGCGTTAAGTGGGGTGGTGCTGGCTTTAAAGACAATGGCAGCAACTGGACGACCATTGGCGGAAGTCGGCAAACC
CTTACCGAGAGTAACCAAATTTTGTTTGATCGTGCCACACAAGCGACTGCAGGCAGTTCAACAATTGACTTGAATGCTGC
TGACGTCGTTAACTTTGGCGTCACCAATACGGCTGCTTCCAGTTTAGCTTTGGGCTATCAAACTGCTGACTATTTGCTCG
ACTTGGAACTTGCTGCAATTGAAACGGATGGTCGCGGTGAAGTGGTGTCGCAGCCCAGAGTCATGACTGCTGATGGTAAG
ACAGCTATGATAGAGTCGGGAACTGAAATCCCTTACCAAGAGGCGGCCTCAAGTGGCGCAACTAGCGTTGCATTTAAGTC
AGCTGTTTTGAAACTTGAGGTGACCCCTCAGATTACTCCAGATGACAGAATTATTATGGATTTGGTTGTTACTAAAGACT
CTGTGGGGGAAAACACTGTTGCGGGGCCCGCGATTGATACTAATGCGGTTGAAACCCAAGTTTTAGTGGATAACGGCGAA
ACTATTGTTTTGGGTGGAATTTTTGAGTCAGAAGAGAGAACGACGATTAATAAAACTCCATTTTTCGGAGATTTACCTCT
GATCGGCGCCCTCTTCCGTTACAAACAAGTTGAGGACAAAAAAACCGAACTCCTGGTCTTCATCACCCCAAAACTCGTGA
AGGATACGCTCGACGCACGCTAG

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(375-437)

AMIN domain (AMIN)

(43-127)

Secretin and TonB N terminus short domain (STN)

(301-347)

Bacterial type II and III secretion system protein (Secretin)

(539-694)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

47.331

100

0.481
  pilQ Acinetobacter baumannii D1279779

40.816

100

0.429
  pilQ Acinetobacter baumannii strain A118

40.816

100

0.429
  comQ Acinetobacter baylyi ADP1

41.279

98.286

0.406
  pilQ Legionella pneumophila strain Lp02

39.678

97.571

0.387
  pilQ Legionella pneumophila strain ERS1305867

39.648

97.286

0.386

Multiple sequence alignment