Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   CHH28_RS01695 Genome accession   NZ_CP022530
Coordinates   370405..371469 (-) Length   354 a.a.
NCBI ID   WP_094061945.1    Uniprot ID   A0A222FRE5
Organism   Bacterioplanes sanyensis strain NV9     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 365405..376469
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CHH28_RS01670 (CHH28_01670) aroK 366059..366577 (-) 519 WP_094058684.1 shikimate kinase AroK -
  CHH28_RS01675 (CHH28_01675) pilQ 366579..368681 (-) 2103 WP_233243705.1 type IV pilus secretin PilQ Machinery gene
  CHH28_RS01680 (CHH28_01680) - 368683..369216 (-) 534 WP_094058685.1 pilus assembly protein PilP -
  CHH28_RS01685 (CHH28_01685) - 369219..369854 (-) 636 WP_233243706.1 type 4a pilus biogenesis protein PilO -
  CHH28_RS01690 (CHH28_01690) - 369854..370405 (-) 552 WP_094058686.1 PilN domain-containing protein -
  CHH28_RS01695 (CHH28_01695) pilM 370405..371469 (-) 1065 WP_094061945.1 pilus assembly protein PilM Machinery gene
  CHH28_RS01700 (CHH28_01700) - 371680..374124 (+) 2445 WP_199243973.1 penicillin-binding protein 1A -
  CHH28_RS01705 (CHH28_01705) - 374195..375598 (-) 1404 WP_199243974.1 FAD-binding oxidoreductase -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38605.35 Da        Isoelectric Point: 4.1684

>NTDB_id=240349 CHH28_RS01695 WP_094061945.1 370405..371469(-) (pilM) [Bacterioplanes sanyensis strain NV9]
MASLFSKKGKAVLGVDISSTSVKVLELSHNNGRYQVEAYASEPLPQNAVVEQAINNDEAVGEAIRRALGRSRSSAKQGAI
AVAGSAVITKTVQMPGDLSDDEMDFQVRAEADQYIPYPLEEVALDWEIQGETEGTPDNMVDVLLAACRSETVERRKDAIE
YANIEAKVVDIEAFCTERAFRLIEGQLDGSEIETVAVIDIGATMSTLNVLHQGKSIYTREQLFGGKQLTEDIMRRYGLSE
EEAQRAKLEGGLPDDYESEVLQPFRNAVVQQVSRSLQFFYSSSQFNDVDYIVLAGGTSSLPDMAELVQDELGVATVVANP
FVNMTLSQKVSANLLNNDAPALMIACGLAMRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=240349 CHH28_RS01695 WP_094061945.1 370405..371469(-) (pilM) [Bacterioplanes sanyensis strain NV9]
CTGGCCAGCTTATTTAGTAAAAAAGGCAAAGCGGTCCTGGGTGTCGACATCAGCTCAACCTCGGTGAAGGTGTTGGAGCT
GAGTCATAACAATGGGCGCTACCAGGTCGAGGCCTATGCTAGTGAACCACTGCCTCAGAACGCTGTCGTCGAACAAGCGA
TTAACAACGACGAAGCCGTCGGTGAAGCCATTCGTCGAGCGCTCGGACGCTCACGCAGTTCTGCCAAGCAAGGTGCCATT
GCTGTTGCTGGTTCCGCTGTGATTACCAAAACCGTGCAGATGCCCGGTGACCTATCTGATGACGAAATGGACTTTCAAGT
CCGTGCCGAAGCTGACCAGTACATTCCTTACCCGTTAGAGGAAGTTGCACTCGACTGGGAGATCCAGGGGGAGACTGAAG
GCACGCCTGACAACATGGTTGATGTGCTGTTGGCAGCTTGTCGCTCTGAAACCGTAGAGCGTCGTAAAGATGCCATCGAA
TACGCCAACATTGAAGCCAAAGTGGTGGATATCGAAGCGTTCTGCACCGAGCGTGCATTCCGCCTCATCGAAGGTCAGTT
GGATGGCAGCGAAATTGAAACGGTGGCTGTGATCGACATCGGTGCCACCATGTCAACTCTCAACGTATTGCATCAAGGTA
AATCGATTTACACGCGTGAACAGCTGTTTGGCGGTAAGCAACTCACCGAAGACATCATGCGTCGTTACGGCCTGAGTGAA
GAAGAAGCGCAGCGCGCGAAGCTCGAGGGTGGCCTGCCCGACGATTATGAATCGGAAGTGCTGCAGCCATTTCGCAATGC
CGTGGTACAGCAAGTGAGCCGTTCACTGCAGTTTTTCTACTCCTCCAGCCAGTTTAATGATGTCGACTATATTGTGTTAG
CTGGCGGTACGTCATCGTTGCCGGACATGGCTGAATTGGTGCAGGACGAGCTGGGAGTTGCCACCGTTGTCGCCAATCCT
TTCGTAAACATGACTCTGTCACAGAAAGTAAGCGCCAATTTGCTTAATAATGACGCGCCAGCATTGATGATCGCATGTGG
GCTGGCGATGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A222FRE5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

53.39

100

0.534
  comM Acinetobacter nosocomialis M2

53.39

100

0.534
  comM Acinetobacter baylyi ADP1

48.87

100

0.489
  pilM Legionella pneumophila strain ERS1305867

43.099

100

0.432

Multiple sequence alignment