Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   CFN79_RS00730 Genome accession   NZ_CP022344
Coordinates   140735..142441 (-) Length   568 a.a.
NCBI ID   WP_104945280.1    Uniprot ID   -
Organism   Chromobacterium vaccinii strain XC0014     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 135735..147441
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CFN79_RS00705 (CFN79_00710) yacG 136963..137154 (-) 192 WP_031297030.1 DNA gyrase inhibitor YacG -
  CFN79_RS00710 (CFN79_00715) zapD 137160..137918 (-) 759 WP_031297029.1 cell division protein ZapD -
  CFN79_RS00715 (CFN79_00720) coaE 137955..138569 (-) 615 WP_104945278.1 dephospho-CoA kinase -
  CFN79_RS00720 (CFN79_00725) - 138554..139462 (-) 909 WP_199776195.1 prepilin peptidase -
  CFN79_RS00725 (CFN79_00730) pilC 139474..140697 (-) 1224 WP_021479152.1 type II secretion system F family protein Machinery gene
  CFN79_RS00730 (CFN79_00735) pilF 140735..142441 (-) 1707 WP_104945280.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CFN79_RS00735 (CFN79_00740) - 142609..143718 (-) 1110 WP_104945281.1 porin -
  CFN79_RS00740 (CFN79_00745) - 144116..144436 (-) 321 WP_021479149.1 ComEA family DNA-binding protein -
  CFN79_RS00745 (CFN79_00750) - 144503..145324 (-) 822 WP_021479148.1 cytochrome C assembly family protein -
  CFN79_RS00750 (CFN79_00755) ffh 145436..146791 (+) 1356 WP_021479147.1 signal recognition particle protein -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62318.93 Da        Isoelectric Point: 5.5625

>NTDB_id=238491 CFN79_RS00730 WP_104945280.1 140735..142441(-) (pilF) [Chromobacterium vaccinii strain XC0014]
MEATAGISGLGRALVQHNMLLQQDADAISKQAVTSHLSFVEQLIQSKKMAARDIAMFSSRVFGYPLLDLASLDTDQLPKG
LVDDEVIASRRLLPLLKRGNKLYIGTSDPTQTSAYHAITFKTGLTVEVVVVEEDKLSRLIGRYQDNSTSALKELESEDFG
DLEFADPEAQLDSGPQTEVDDAPVVKFIHKMLLDAIGLGASDIHFEPYEKYYRIRYRVDGVLKEVAQPPLLLKEKIASRI
KVISRLDISEKRLPQDGRLKLVISKTRAIDFRVSTLPTLFGEKIVMRILDSSAASLDIDQLGFEPEQKAHLLRAIARPYG
MVLVTGPTGSGKTVSLYTCLNILNKPDTNISTAEDPVEINLPGINQVNVNEKAGLTFASALKAFLRQDPDVIMVGEIRDF
ETADIAIKAAQTGHMVFSTLHTNDAPSTLTRMLNMGVAPFNIASSVLLIMAQRLARKLCSHCKQPVEIPEEALLRAGFLK
EELAGGWHPYGPVGCEECRNTGYRGRVGIFEVMPISDAMMRLIMKNGTAVDISDLAKEEGMVDLRRAGLLKVIAGMTSLA
EVEAVTND

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=238491 CFN79_RS00730 WP_104945280.1 140735..142441(-) (pilF) [Chromobacterium vaccinii strain XC0014]
ATGGAAGCTACCGCAGGGATTTCCGGCCTGGGCAGGGCCCTGGTCCAGCACAATATGCTGTTGCAGCAGGATGCGGACGC
CATTTCGAAACAGGCGGTCACTTCCCATCTCAGTTTTGTCGAGCAGCTGATCCAGAGCAAGAAGATGGCCGCGCGCGACA
TCGCGATGTTTTCATCCCGGGTGTTCGGCTACCCGTTGCTGGATCTGGCTTCGCTGGATACCGACCAGCTGCCCAAAGGG
TTGGTGGATGATGAAGTGATCGCCAGCCGTCGGTTGCTGCCGCTGCTCAAGCGCGGCAACAAGCTTTACATCGGCACTTC
CGATCCTACCCAGACTTCCGCCTACCATGCGATCACTTTCAAGACCGGCCTGACGGTAGAAGTGGTAGTGGTGGAGGAGG
ACAAGCTGTCCCGACTGATCGGACGGTATCAGGACAATTCGACCTCGGCCTTGAAAGAGCTGGAGAGCGAGGATTTCGGC
GATCTGGAATTCGCCGATCCCGAGGCGCAACTGGACAGTGGTCCGCAGACCGAGGTCGACGACGCGCCGGTGGTGAAGTT
CATCCACAAGATGTTGCTGGATGCGATAGGCCTCGGCGCGTCCGACATCCACTTCGAGCCATACGAGAAGTACTATCGCA
TCCGCTACCGGGTGGATGGCGTATTGAAGGAGGTGGCGCAGCCGCCGTTGCTGCTGAAGGAAAAGATCGCGTCCCGCATC
AAGGTGATTTCCAGGCTGGATATTTCGGAAAAGCGGCTGCCTCAGGATGGCCGGCTGAAACTGGTGATTTCCAAGACCCG
GGCGATAGATTTCCGCGTCAGCACCTTGCCCACCTTGTTCGGCGAGAAAATCGTGATGCGTATCCTGGATTCGTCGGCAG
CCTCGCTGGATATAGACCAGCTGGGCTTCGAACCGGAGCAAAAAGCGCACCTGTTGAGGGCGATCGCCCGGCCCTACGGC
ATGGTGCTGGTGACCGGCCCCACCGGCAGCGGTAAGACCGTATCGCTTTACACTTGCCTGAACATTTTGAACAAGCCGGA
CACGAATATCTCCACCGCGGAAGATCCGGTGGAAATCAACCTGCCGGGCATCAATCAGGTCAACGTCAACGAAAAGGCGG
GATTGACCTTCGCTTCGGCGTTGAAGGCTTTTCTGCGGCAAGATCCTGACGTGATCATGGTGGGCGAGATTCGCGATTTC
GAGACCGCCGACATCGCGATCAAGGCCGCGCAGACCGGCCACATGGTGTTCTCGACGCTGCATACCAACGACGCGCCGTC
CACCTTGACGCGGATGCTGAACATGGGCGTGGCGCCGTTCAACATCGCCAGTTCGGTGTTGCTGATCATGGCGCAGCGCT
TGGCGCGCAAACTATGCAGCCATTGCAAGCAGCCGGTGGAGATTCCCGAGGAGGCACTGCTGCGCGCAGGTTTCTTGAAG
GAGGAGTTGGCCGGCGGCTGGCACCCCTACGGGCCGGTCGGCTGCGAGGAATGCCGGAACACCGGCTACAGGGGGCGCGT
CGGGATTTTCGAAGTGATGCCGATCAGCGATGCGATGATGCGGCTGATCATGAAGAACGGCACTGCGGTGGACATCTCCG
ATCTGGCCAAGGAGGAGGGCATGGTCGATCTGCGCCGCGCCGGCCTGTTGAAGGTGATTGCCGGCATGACCTCGCTGGCC
GAGGTCGAGGCGGTGACCAACGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-148)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

58.053

99.472

0.577
  pilB Acinetobacter baumannii D1279779

54.452

100

0.56
  pilB Legionella pneumophila strain ERS1305867

53.671

100

0.54
  pilB Acinetobacter baylyi ADP1

54.352

99.12

0.539
  pilB Vibrio cholerae strain A1552

50.089

98.415

0.493
  pilB Vibrio parahaemolyticus RIMD 2210633

48.49

99.12

0.481
  pilB Vibrio campbellii strain DS40M4

47.069

99.12

0.467
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.674

97.183

0.386
  pilF Thermus thermophilus HB27

38.256

98.944

0.379

Multiple sequence alignment