Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   CFH90_RS03945 Genome accession   NZ_CP022298
Coordinates   782909..784030 (-) Length   373 a.a.
NCBI ID   WP_126035492.1    Uniprot ID   A0A3Q8XD77
Organism   Acinetobacter johnsonii strain IC001     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 777909..789030
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CFH90_RS03915 (CFH90_03905) - 778728..779576 (+) 849 WP_126035487.1 class II glutamine amidotransferase -
  CFH90_RS03920 (CFH90_03910) - 779851..780306 (+) 456 WP_078389448.1 bacteriohemerythrin -
  CFH90_RS03925 (CFH90_03915) - 780449..781531 (+) 1083 WP_126035489.1 hypothetical protein -
  CFH90_RS03930 (CFH90_03920) - 781531..781854 (+) 324 WP_104222298.1 RnfH family protein -
  CFH90_RS03935 (CFH90_03925) - 781901..782299 (-) 399 WP_004694868.1 outer membrane protein assembly factor BamE -
  CFH90_RS03940 (CFH90_03930) fur 782412..782849 (+) 438 WP_004694866.1 ferric iron uptake transcriptional regulator -
  CFH90_RS03945 (CFH90_03935) pilU 782909..784030 (-) 1122 WP_126035492.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CFH90_RS03950 (CFH90_03940) pilT 784057..785094 (-) 1038 WP_004694862.1 type IV pilus twitching motility protein PilT Machinery gene
  CFH90_RS03955 (CFH90_03945) - 785217..785906 (+) 690 WP_126035494.1 YggS family pyridoxal phosphate-dependent enzyme -
  CFH90_RS03960 (CFH90_03950) - 786090..786824 (+) 735 WP_126035496.1 hypothetical protein -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41875.16 Da        Isoelectric Point: 6.6662

>NTDB_id=238191 CFH90_RS03945 WP_126035492.1 782909..784030(-) (pilU) [Acinetobacter johnsonii strain IC001]
MDFNDLLNYMIQQKASDLFITADVEPSMKINGQIVPVAKGKLTGDVAGQLLNSIMSDKQRKEFADTRECNFAIMNRDKTA
RFRVSAFQQRDMPGMVLRRIETQIPTMDELKLPPVLKELAMTKRGIIIFVGATGTGKSTSLASIIGYRNQNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSRETMDYAIAFAETGHLVFATLHANNANQAIDR
IIHFFEADRHSQLFMDLSLNLKAMVAQQLIPTPDGNSRRAAIEILINSPLISDLIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAKQITYKDALKHADSPNDLRLQIKLSEEGGDRLLHASPNITFDGQA

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=238191 CFH90_RS03945 WP_126035492.1 782909..784030(-) (pilU) [Acinetobacter johnsonii strain IC001]
ATGGATTTTAACGACTTACTCAATTATATGATTCAACAAAAAGCGTCGGATTTATTTATCACCGCTGATGTTGAGCCCTC
CATGAAAATTAACGGTCAAATTGTGCCTGTGGCTAAGGGTAAGCTGACGGGTGATGTCGCGGGGCAACTGCTCAACTCGA
TTATGAGTGACAAGCAGCGTAAAGAATTTGCGGACACACGTGAGTGCAACTTCGCAATTATGAATCGGGATAAAACTGCA
CGTTTTCGTGTCAGTGCCTTTCAACAGCGTGATATGCCAGGTATGGTTTTGCGTCGAATTGAGACGCAAATTCCAACCAT
GGATGAGTTAAAACTCCCGCCTGTACTCAAAGAGTTAGCCATGACCAAACGTGGCATCATCATCTTTGTGGGAGCAACAG
GTACGGGTAAATCGACTTCTTTGGCTTCGATCATTGGTTACCGTAACCAAAACTCGAAAGGCCATATCATTACCATTGAA
GACCCAATTGAATTTATTCATGAGCATGCAGGCTGTATCATTACCCAACGTGAAGTGGGCATTGATACTGACTCTTTTGA
AATCGCACTGAAAAATACCTTACGTCAAGCACCCGATGTGATCCTGATTGGTGAGATTCGTTCCCGTGAAACCATGGATT
ATGCGATTGCCTTTGCTGAAACAGGTCACTTGGTATTTGCAACGCTACACGCCAACAACGCCAACCAAGCCATTGACCGT
ATTATTCACTTCTTTGAAGCAGACCGTCATAGCCAATTGTTTATGGACTTGTCTTTAAACCTTAAAGCGATGGTTGCTCA
GCAGTTGATTCCAACACCAGACGGTAATTCACGTCGCGCTGCAATTGAGATTTTGATTAACTCACCGTTAATTTCCGATT
TGATCCGTAAAGGTGAAATCCACGAAATTAAAGACCTGATGAAGCGTTCTCGTGAACTCGGTATGCAAACCTTTGACCAA
GCCTTATTCGATTTGTACAAAGCTAAGCAGATTACCTATAAAGATGCGCTGAAACACGCCGACTCTCCAAACGACTTACG
CTTACAAATCAAGTTGTCTGAAGAAGGCGGTGATCGTTTATTGCATGCCAGTCCAAACATCACGTTTGACGGACAAGCTT
AA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-250)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3Q8XD77

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

76.72

100

0.777
  pilU Pseudomonas stutzeri DSM 10701

67.925

99.464

0.676
  pilU Vibrio cholerae strain A1552

55.405

99.196

0.55
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.463

98.928

0.41
  pilT Legionella pneumophila strain Lp02

41.42

90.617

0.375
  pilT Legionella pneumophila strain ERS1305867

41.42

90.617

0.375
  pilT Pseudomonas aeruginosa PAK

39.306

92.761

0.365

Multiple sequence alignment