Detailed information NTG0238180

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: CFH90_RS01800
• Genome accession: NZ_CP022298
• Coordinates: 368756..369814 (+)
• Length: 352 a.a.
• NCBI ID: WP_058870420.1
• Uniprot ID: A0A345B4L2
• Organism: Acinetobacter johnsonii strain IC001
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 363756..374814

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CFH90_RS01785 (CFH90_01770)	mraY	364035..365153 (+)	1119	WP_126035041.1	phospho-N-acetylmuramoyl-pentapeptide- transferase	-
CFH90_RS01790 (CFH90_01775)	-	365224..366030 (+)	807	WP_126035043.1	putative RNA methyltransferase	-
CFH90_RS01795 (CFH90_01780)	ponA	366062..368590 (-)	2529	WP_126035045.1	penicillin-binding protein PBP1a	-
CFH90_RS01800 (CFH90_01785)	pilM	368756..369814 (+)	1059	WP_058870420.1	pilus assembly protein PilM	Machinery gene
CFH90_RS01805 (CFH90_01790)	pilN	369814..370470 (+)	657	WP_004691498.1	PilN domain-containing protein	Machinery gene
CFH90_RS01810 (CFH90_01795)	pilO	370467..371189 (+)	723	WP_126035047.1	type 4a pilus biogenesis protein PilO	Machinery gene
CFH90_RS01815 (CFH90_01800)	pilP	371189..371716 (+)	528	WP_126035049.1	pilus assembly protein PilP	Machinery gene
CFH90_RS01820 (CFH90_01805)	pilQ	371781..373814 (+)	2034	WP_410169713.1	type IV pilus secretin PilQ	Machinery gene
CFH90_RS01825 (CFH90_01810)	aroK	373852..374385 (+)	534	WP_125280899.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 38953.50 Da
• Isoelectric Point: 4.4181

>NTDB_id=238180 CFH90_RS01800 WP_058870420.1 368756..369814(+) (pilM) [Acinetobacter johnsonii strain IC001]
MLRLYRKPSKGLIGVDISSTSVKVLELSVKNNRYWVESYALVPLPEGSVVEKNILNPEAVGDALERAVNLANVQSTDVAL
AVPTSMVITKIIEMDADMNDDEREIQIRDDAEQYIPFPLDEASLDFEVLPDRLANPNRVNVLLVATRIENVEARSEALEL
GGLTPKIADVESFAIENAFKVFSDTLPMGVNTVGILDIGHTMTTLSVMQNNKVIYTREQVFGGKQLTQEIQNRYGLSFEE
AGRAKKSRTLPDDYDIEVLEPFLEAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQIDIKKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=238180 CFH90_RS01800 WP_058870420.1 368756..369814(+) (pilM) [Acinetobacter johnsonii strain IC001]
GTGCTCAGGTTATATCGTAAGCCAAGTAAGGGGTTAATAGGAGTAGATATTAGTTCGACTTCTGTTAAGGTATTGGAACT
TTCTGTTAAAAACAATCGGTATTGGGTGGAGAGCTATGCATTAGTCCCATTACCAGAAGGAAGTGTGGTCGAAAAAAATA
TATTGAATCCAGAAGCAGTTGGTGATGCCCTTGAGCGTGCGGTAAATTTGGCCAATGTTCAATCGACAGACGTAGCACTG
GCAGTTCCGACGTCCATGGTGATTACTAAAATCATTGAAATGGATGCCGATATGAACGATGACGAACGTGAAATTCAGAT
TCGTGATGATGCTGAGCAATATATTCCATTCCCATTAGATGAAGCGAGTTTGGACTTTGAAGTCCTTCCAGACCGTTTAG
CCAATCCCAATCGTGTCAATGTACTCCTCGTTGCAACCCGCATTGAAAATGTAGAGGCGCGTTCCGAAGCATTGGAGTTA
GGCGGTTTAACGCCTAAAATTGCCGATGTTGAAAGTTTCGCCATTGAAAATGCATTCAAAGTCTTTTCGGATACGCTTCC
AATGGGTGTAAACACCGTTGGGATTCTGGACATCGGTCATACCATGACGACCTTATCTGTCATGCAGAATAATAAAGTCA
TTTATACCCGTGAACAGGTGTTCGGTGGTAAACAACTTACTCAAGAAATTCAAAACCGCTATGGTTTGTCTTTTGAAGAA
GCTGGACGTGCCAAAAAAAGCCGTACCTTGCCTGACGATTATGATATTGAAGTGCTTGAACCTTTTCTTGAAGCGGTGGT
GCAACAAGCAGCACGTTCTTTGCAGTTCTTTTTTTCGTCCTCTCAATTTAATGAGATCGACCATATTTTATTGGCAGGGG
GGAATGCCAATATTCCAGGACTAGCGAAGTTATTACAACAAAAATTAGGTTATCGGGTCACTATTGCCAACCCATTTTTA
CAGATGGGCTTTTCTCCACAAATCGACATTAAAAAAATTGAAAATGATGCTTCATCACTCATGGTGGCATGTGGATTGGC
ATTGAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: A0A345B4L2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	85.227	100	0.852
comM	Acinetobacter nosocomialis M2	85.227	100	0.852
comM	Acinetobacter baylyi ADP1	80.398	100	0.804
pilM	Legionella pneumophila strain ERS1305867	40.96	100	0.412