Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   CFH90_RS01810 Genome accession   NZ_CP022298
Coordinates   370467..371189 (+) Length   240 a.a.
NCBI ID   WP_126035047.1    Uniprot ID   A0A3S9AGT4
Organism   Acinetobacter johnsonii strain IC001     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 365467..376189
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CFH90_RS01795 (CFH90_01780) ponA 366062..368590 (-) 2529 WP_126035045.1 penicillin-binding protein PBP1a -
  CFH90_RS01800 (CFH90_01785) pilM 368756..369814 (+) 1059 WP_058870420.1 pilus assembly protein PilM Machinery gene
  CFH90_RS01805 (CFH90_01790) pilN 369814..370470 (+) 657 WP_004691498.1 PilN domain-containing protein Machinery gene
  CFH90_RS01810 (CFH90_01795) pilO 370467..371189 (+) 723 WP_126035047.1 type 4a pilus biogenesis protein PilO Machinery gene
  CFH90_RS01815 (CFH90_01800) pilP 371189..371716 (+) 528 WP_126035049.1 pilus assembly protein PilP Machinery gene
  CFH90_RS01820 (CFH90_01805) pilQ 371781..373814 (+) 2034 WP_410169713.1 type IV pilus secretin PilQ Machinery gene
  CFH90_RS01825 (CFH90_01810) aroK 373852..374385 (+) 534 WP_125280899.1 shikimate kinase AroK -
  CFH90_RS01830 (CFH90_01815) aroB 374416..375501 (+) 1086 WP_126035053.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 240 a.a.        Molecular weight: 27082.53 Da        Isoelectric Point: 4.8752

>NTDB_id=238182 CFH90_RS01810 WP_126035047.1 370467..371189(+) (pilO) [Acinetobacter johnsonii strain IC001]
MSRDEFEELTSENPAVTKKKMTVDKFFQQFNTLDPNNYGSWPFSVKLTCWIFIILVIGALGYFVVIKPKLDAISNAHAQE
QNLLNEFREKDSKLRNLQQYQAQLQEMEANFNQQLEQLPKETEIPGLVEDINVSGVNSGLKFKNIRLEPEIRQEFFIEQP
ISIEATGDYHSFGSFVSGIAALPRIVTLHDFDISASSNNEKKTDIPQISYSVKAKTYRYVGATAATDVENTTKADTGGAK

Nucleotide


Download         Length: 723 bp        

>NTDB_id=238182 CFH90_RS01810 WP_126035047.1 370467..371189(+) (pilO) [Acinetobacter johnsonii strain IC001]
ATGAGTCGCGATGAATTTGAAGAATTAACATCAGAAAACCCTGCTGTAACCAAGAAAAAAATGACGGTTGATAAATTTTT
TCAACAGTTTAATACCTTAGATCCAAACAATTACGGCAGTTGGCCCTTTTCAGTCAAATTGACTTGTTGGATCTTTATCA
TTTTAGTGATTGGAGCTTTGGGTTATTTTGTCGTGATTAAGCCGAAGCTTGATGCGATTTCAAATGCACATGCACAAGAG
CAAAATTTACTGAATGAGTTTCGTGAAAAAGATTCAAAATTACGTAACTTGCAGCAATATCAAGCGCAATTGCAAGAAAT
GGAAGCTAATTTTAATCAGCAGTTAGAACAGCTGCCGAAAGAAACTGAAATTCCTGGCTTGGTTGAAGATATCAACGTGA
GCGGCGTAAATTCTGGATTAAAGTTTAAAAATATTCGTTTAGAACCAGAAATTCGCCAAGAGTTCTTTATCGAACAGCCG
ATTTCCATTGAAGCGACGGGTGATTATCATTCTTTTGGTTCATTTGTCAGTGGAATTGCCGCTTTACCGCGTATTGTGAC
CTTGCATGACTTCGATATCTCAGCATCAAGTAACAATGAGAAAAAGACGGATATTCCGCAAATTTCATACAGTGTAAAAG
CAAAAACCTACCGTTATGTCGGGGCGACAGCTGCTACAGATGTAGAAAATACAACTAAAGCAGATACAGGAGGTGCCAAG
TAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(74-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3S9AGT4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

71.967

99.583

0.717
  comO Acinetobacter baylyi ADP1

68.443

100

0.696

Multiple sequence alignment