Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   MS_RS01650 Genome accession   NC_006300
Coordinates   335204..336604 (-) Length   466 a.a.
NCBI ID   WP_041639505.1    Uniprot ID   -
Organism   [Mannheimia] succiniciproducens MBEL55E     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 330204..341604
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MS_RS01620 (MS0353) - 330320..331711 (-) 1392 WP_011199535.1 alanine/glycine:cation symporter family protein -
  MS_RS01625 (MS0357) - 332121..332393 (-) 273 WP_011199539.1 GNAT family N-acetyltransferase -
  MS_RS01630 (MS0358) yacG 332410..332628 (-) 219 WP_011199540.1 DNA gyrase inhibitor YacG -
  MS_RS01635 (MS0359) coaE 332606..333250 (-) 645 WP_011199541.1 dephospho-CoA kinase -
  MS_RS01640 (MS0360) - 333277..333993 (-) 717 WP_011199542.1 prepilin peptidase -
  MS_RS01645 (MS0361) pilC 333990..335207 (-) 1218 WP_041639504.1 type II secretion system F family protein Machinery gene
  MS_RS01650 (MS0363) pilB 335204..336604 (-) 1401 WP_041639505.1 GspE/PulE family protein Machinery gene
  MS_RS01655 (MS0364) pilA 336618..337070 (-) 453 WP_011199546.1 prepilin peptidase-dependent pilin Machinery gene
  MS_RS01660 (MS0365) ampD 337174..337746 (+) 573 WP_011199547.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  MS_RS01665 (MS0366) - 337884..338354 (+) 471 WP_011199548.1 surface-adhesin E family protein -
  MS_RS01670 (MS0367) era 338429..339382 (-) 954 WP_011199549.1 GTPase Era -
  MS_RS01675 (MS0368) rnc 339379..340053 (-) 675 WP_011199550.1 ribonuclease III -
  MS_RS01680 (MS0369) lepB 340055..340777 (-) 723 WP_041639506.1 signal peptidase I -

Sequence

Protein


Download         Length: 466 a.a.        Molecular weight: 53213.86 Da        Isoelectric Point: 6.7676

>NTDB_id=23660 MS_RS01650 WP_041639505.1 335204..336604(-) (pilB) [[Mannheimia] succiniciproducens MBEL55E]
MQSIKIIAGNGEQYMIDHELWQRNQQQQHVLLRYLAVPIKEEEQKLWLAIDNVENIAACETFSFLTGKIVEPVLVSNETL
KSLLQPDEPQSLSIEETSLIYTESLSQNKENKNTDEPIIRLLNNIFESALSKNASDIHFEPQKNQLRIRFRIDGVLQQQT
PVNLSLAGRIISRLKLLAKLDISETRLPQDGRFDFKTTFAETLDFRISTLATSNGEKIVLRLQQNKPVDFSFEQLGMEPA
QQQKLEQALNQPQGLILVTGPTGSGKSITLYTALQWLNSASKHIMTAEDPIEIELEGIIQTQIQPQIGLSFSRLLRTFLR
QDPDVIMLGEIRDDESAQMALRAAQTGHLVLSTLHTNNAYSAISRLLQLGIKQHEIDNSLLLIIAQRLVRKRCQKCGQFS
ENFINCDCHQGYRGRIGIYQFLHPRWQAQKWQYVTDFPSLYQAAKNKVQQQITDKQELLRVLGSEK

Nucleotide


Download         Length: 1401 bp        

>NTDB_id=23660 MS_RS01650 WP_041639505.1 335204..336604(-) (pilB) [[Mannheimia] succiniciproducens MBEL55E]
ATGCAATCAATTAAAATCATTGCCGGCAATGGCGAACAATACATGATTGATCACGAATTGTGGCAACGCAATCAACAGCA
GCAACATGTATTGTTACGCTATCTTGCCGTACCCATCAAAGAGGAAGAGCAAAAACTATGGCTTGCCATTGATAATGTAG
AAAATATCGCCGCTTGCGAAACCTTTTCCTTTTTAACCGGCAAAATTGTTGAACCCGTATTAGTCAGTAACGAAACCTTA
AAATCATTATTGCAACCGGATGAACCGCAATCATTAAGTATTGAAGAAACATCATTGATTTATACGGAAAGCCTCTCTCA
AAATAAGGAAAATAAAAATACCGACGAACCTATTATCCGTTTATTAAATAATATTTTTGAGAGCGCCCTAAGTAAAAATG
CCTCCGATATTCATTTCGAGCCGCAAAAAAACCAGCTTCGGATTCGTTTTAGGATTGACGGTGTGTTGCAACAACAAACA
CCCGTTAATCTTTCTTTAGCAGGGCGCATTATTTCAAGATTAAAACTGTTGGCAAAATTAGATATCAGCGAAACCCGCTT
ACCGCAAGACGGACGTTTTGATTTCAAAACCACCTTCGCCGAAACCCTGGATTTTCGTATATCGACCCTTGCCACCAGTA
ACGGTGAAAAAATCGTGCTGCGTTTACAACAAAATAAACCGGTGGATTTCAGCTTTGAACAACTTGGTATGGAGCCTGCG
CAACAACAAAAACTCGAACAAGCACTTAATCAGCCGCAAGGCTTAATTTTAGTGACCGGCCCGACCGGTAGCGGGAAAAG
TATCACCCTTTATACGGCGCTGCAATGGCTTAATAGCGCCTCAAAGCATATAATGACGGCGGAAGATCCCATTGAAATTG
AACTTGAAGGGATTATTCAAACCCAAATACAGCCTCAAATAGGCTTGAGTTTCAGCCGACTGCTGCGCACATTTCTACGT
CAGGATCCGGATGTCATTATGCTAGGTGAAATCCGTGATGATGAAAGTGCTCAAATGGCATTACGTGCAGCGCAAACCGG
GCATTTGGTTCTATCCACCTTGCATACTAACAATGCTTACTCCGCAATTTCACGCTTATTGCAACTGGGAATTAAACAGC
ATGAAATCGATAATAGCCTGCTCCTTATTATCGCCCAACGCTTAGTGCGAAAGCGTTGCCAAAAGTGCGGTCAATTTTCC
GAAAATTTTATCAACTGCGACTGTCATCAAGGTTACCGAGGACGTATCGGCATTTATCAGTTTCTTCATCCCCGGTGGCA
AGCTCAGAAATGGCAATATGTCACGGATTTTCCTTCCCTTTATCAGGCGGCAAAAAACAAAGTACAACAACAAATTACCG
ATAAGCAGGAATTATTGCGCGTATTAGGAAGTGAAAAATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(12-85)

Type II/IV secretion system protein (T2SSE)

(124-391)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

66.304

98.712

0.655
  pilB Haemophilus influenzae Rd KW20

65.795

98.498

0.648
  pilB Glaesserella parasuis strain SC1401

60

96.567

0.579
  pilB Vibrio parahaemolyticus RIMD 2210633

41.494

100

0.429
  pilB Vibrio campbellii strain DS40M4

41.372

100

0.427
  pilB Vibrio cholerae strain A1552

40.491

100

0.425
  pilB Legionella pneumophila strain ERS1305867

38.833

100

0.414
  pilB Acinetobacter baylyi ADP1

37.876

100

0.406
  pilB Acinetobacter baumannii D1279779

38.046

100

0.393

Multiple sequence alignment