Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   MS_RS01655 Genome accession   NC_006300
Coordinates   336618..337070 (-) Length   150 a.a.
NCBI ID   WP_011199546.1    Uniprot ID   Q65VN9
Organism   [Mannheimia] succiniciproducens MBEL55E     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 331618..342070
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MS_RS01625 (MS0357) - 332121..332393 (-) 273 WP_011199539.1 GNAT family N-acetyltransferase -
  MS_RS01630 (MS0358) yacG 332410..332628 (-) 219 WP_011199540.1 DNA gyrase inhibitor YacG -
  MS_RS01635 (MS0359) coaE 332606..333250 (-) 645 WP_011199541.1 dephospho-CoA kinase -
  MS_RS01640 (MS0360) - 333277..333993 (-) 717 WP_011199542.1 prepilin peptidase -
  MS_RS01645 (MS0361) pilC 333990..335207 (-) 1218 WP_041639504.1 type II secretion system F family protein Machinery gene
  MS_RS01650 (MS0363) pilB 335204..336604 (-) 1401 WP_041639505.1 GspE/PulE family protein Machinery gene
  MS_RS01655 (MS0364) pilA 336618..337070 (-) 453 WP_011199546.1 prepilin peptidase-dependent pilin Machinery gene
  MS_RS01660 (MS0365) ampD 337174..337746 (+) 573 WP_011199547.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  MS_RS01665 (MS0366) - 337884..338354 (+) 471 WP_011199548.1 surface-adhesin E family protein -
  MS_RS01670 (MS0367) era 338429..339382 (-) 954 WP_011199549.1 GTPase Era -
  MS_RS01675 (MS0368) rnc 339379..340053 (-) 675 WP_011199550.1 ribonuclease III -
  MS_RS01680 (MS0369) lepB 340055..340777 (-) 723 WP_041639506.1 signal peptidase I -
  MS_RS01685 (MS0370) lepB 340793..341815 (-) 1023 WP_041639508.1 signal peptidase I -

Sequence

Protein


Download         Length: 150 a.a.        Molecular weight: 15845.14 Da        Isoelectric Point: 8.4444

>NTDB_id=23661 MS_RS01655 WP_011199546.1 336618..337070(-) (pilA) [[Mannheimia] succiniciproducens MBEL55E]
MKNYRTLSQYPAIRKGFTLIELMIVIAIIAILATIAIPSYQNYTKRAAISELLQAGAPYKADVELCIYEKGGEKDCSSGA
NGIAEPAKTKGKYVDAVTVSSGTISVTGKGSLSGVSYSTKATGNASEGISWTTTCTPKDIFPAGFCDNKE

Nucleotide


Download         Length: 453 bp        

>NTDB_id=23661 MS_RS01655 WP_011199546.1 336618..337070(-) (pilA) [[Mannheimia] succiniciproducens MBEL55E]
ATGAAAAATTACCGCACTTTATCTCAGTACCCTGCCATTCGCAAAGGTTTTACACTGATTGAATTAATGATTGTTATCGC
TATTATTGCCATTCTCGCTACTATTGCAATTCCCTCTTATCAAAATTACACCAAACGGGCGGCAATTTCCGAATTATTGC
AGGCGGGCGCACCCTATAAAGCCGATGTGGAATTATGTATTTATGAAAAAGGCGGTGAAAAAGACTGCTCAAGCGGTGCC
AACGGTATTGCCGAACCGGCGAAAACAAAAGGGAAATACGTTGATGCCGTCACCGTGAGCAGTGGTACGATTTCCGTTAC
CGGTAAAGGCAGTTTATCCGGTGTCAGTTATTCGACCAAAGCTACCGGTAATGCTTCGGAAGGTATCAGTTGGACGACAA
CCTGCACGCCTAAAGATATTTTCCCGGCAGGTTTTTGTGATAATAAAGAATAG

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(14-36)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q65VN9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

61.268

94.667

0.58
  pilA Haemophilus influenzae Rd KW20

60.563

94.667

0.573
  pilA Glaesserella parasuis strain SC1401

52.258

100

0.54
  pilA Acinetobacter nosocomialis M2

41.429

93.333

0.387

Multiple sequence alignment